Nutritional regulation of stem and progenitor cells in <i>Drosophila</i>

Shim, Jiwon; Rao, Shubha Gururaja; Banerjee, Utpal

doi:10.1242/dev.079087

Cited by 85 publications

(98 citation statements)

References 123 publications

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“…Drosophila melanogaster is emerging as a powerful model in which to study hormonal control of growth, metabolism and development (reviewed by Padmanabha and Baker, 2014;Shim et al, 2013;Tennessen and Thummel, 2011). Ecdysteroids -the steroid hormones of arthropods -are key regulators of a network of interorgan communication that triggers molting and regulates growth, metabolism and fertility (reviewed by Lafont et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol.

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Section: Introductionmentioning

confidence: 99%

The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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“…In addition, a small group of cells termed the posterior signaling center (PSC) controls differentiation and specification of hemocytes in the CZ (Benmimoun et al, 2015;Oyallon et al, 2016). Although much remains to be understood, the fly LG has developed into a powerful model for hematopoiesis and stem cell/progenitor regulation (Crozatier and Meister, 2007;Crozatier and Vincent, 2011;Letourneau et al, 2016;Martinez-Agosto et al, 2007;MorinPoulard et al, 2013;Shim et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

The matrix protein Tiggrin regulates plasmatocyte maturation inDrosophilalarva

Zhang

Cadigan

2017

Development

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The lymph gland (LG) is a major source of hematopoiesis during Drosophila development. In this tissue, prohemocytes differentiate into multiple lineages, including macrophage-like plasmatocytes, which comprise the vast majority of mature hemocytes. Previous studies have uncovered genetic pathways that regulate prohemocyte maintenance and some cell fate choices between hemocyte lineages. However, less is known about how the plasmatocyte pool of the LG is established and matures. Here, we report that Tiggrin, a matrix protein expressed in the LG, is a specific regulator of plasmatocyte maturation. Tiggrin mutants exhibit precocious maturation of plasmatocytes, whereas Tiggrin overexpression blocks this process, resulting in a buildup of intermediate progenitors (IPs) expressing prohemocyte and hemocyte markers. These IPs likely represent a transitory state in prohemocyte to plasmatocyte differentiation. We also found that overexpression of Wee1 kinase, which slows G 2 /M progression, results in a phenotype similar to Tiggrin overexpression, whereas String/Cdc25 expression phenocopies Tiggrin mutants. Further analysis revealed that Wee1 inhibits plasmatocyte maturation through upregulation of Tiggrin transcription. Our results elucidate connections between the extracellular matrix and cell cycle regulators in the regulation of hematopoiesis.

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“…Similarly, Hippo signaling maintains lung cell homeostasis by controlling the proliferation of epithelial stem cells (Lange et al 2015). Although some factors that regulate stem cell proliferation have been widely studied (Brack et al 2008;Takashima et al 2008;Benmimoun et al 2012;Chen et al 2012;Shim et al 2013), additional components and detailed mechanisms controlling stem cell proliferation are not clearly understood.…”

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confidence: 99%

Adult Muscle Formation Requires Drosophila Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors

et al. 2017

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Adult muscle precursor (AMP) cells located in the notum of the larval wing disc undergo rapid amplification and eventual fusion to generate the Drosophila melanogaster indirect flight muscles (IFMs). Here we find that loss of Moleskin (Msk) function in these wing disc-associated myoblasts reduces the overall AMP pool size, resulting in the absence of IFM formation. This myoblast loss is due to a decrease in the AMP proliferative capacity and is independent of cell death. In contrast, disruption of Msk during pupal myoblast proliferation does not alter the AMP number, suggesting that Msk is specifically required for larval AMP proliferation. It has been previously shown that Wingless (Wg) signaling maintains expression of the Vestigial (Vg) transcription factor in proliferating myoblasts. However, other factors that influence Wg-mediated myoblast proliferation are largely unknown. Here we examine the interactions between Msk and the Wg pathway in regulation of the AMP pool size. We find that a myoblast-specific reduction of Msk results in the absence of Vg expression and a complete loss of the Wg pathway readout b-catenin/Armadillo (Arm). Moreover, msk RNA interference knockdown abolishes expression of the Wg target Ladybird (Lbe) in leg disc myoblasts. Collectively, our results provide strong evidence that Msk acts through the Wg signaling pathway to control myoblast pool size and muscle formation by regulating Arm stability or nuclear transport.

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Nutritional regulation of stem and progenitor cells in Drosophila

Cited by 85 publications

References 123 publications

The Ecdysteroidome of Drosophila: influence of diet and development

The Ecdysteroidome of Drosophila: influence of diet and development

The matrix protein Tiggrin regulates plasmatocyte maturation inDrosophilalarva

Adult Muscle Formation Requires Drosophila Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors

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