Adult Muscle Formation Requires <i>Drosophila</i> Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors

Vishal, Kumar; Brooks, David; Bawa, Simranjot; Gameros, Samantha; Stetsiv, Marta; Geisbrecht, Erika R.

doi:10.1534/genetics.116.193813

Cited by 8 publications

(9 citation statements)

References 61 publications

(71 reference statements)

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“…Wing disc cell density was quantified by counting the total number of nuclei stained with DAPI in five random regions (430 μm 2 area each) of the wing imaginal disc. This was done for five different wing discs ( 68 ).…”

Section: Methodsmentioning

confidence: 99%

Immune Control of Animal Growth in Homeostasis and Nutritional Stress in Drosophila

et al. 2020

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A large body of research implicates the brain and fat body (liver equivalent) as central players in coordinating growth and nutritional homeostasis in multicellular animals. In this regard, an underlying connection between immune cells and growth is also evident, although mechanistic understanding of this cross-talk is scarce. Here, we explore the importance of innate immune cells in animal growth during homeostasis and in conditions of nutrient stress. We report that Drosophila larvae lacking blood cells eclose as small adults and show signs of insulin insensitivity. Moreover, when exposed to dietary stress of a high-sucrose diet (HSD), these animals are further growth retarded than normally seen in regular animals raised on HSD. In contrast, larvae carrying increased number of activated macrophage-like plasmatocytes show no defects in adult growth when raised on HSD and grow to sizes almost comparable with that seen with regular diet. These observations imply a central role for immune cell activity in growth control. Mechanistically, our findings reveal a surprising influence of immune cells on balancing fat body inflammation and insulin signaling under conditions of homeostasis and nutrient overload as a means to coordinate systemic metabolism and adult growth. This work integrates both the cellular and humoral arm of the innate immune system in organismal growth homeostasis, the implications of which may be broadly conserved across mammalian systems as well.

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Section: Methodsmentioning

confidence: 99%

Immune Control of Animal Growth in Homeostasis and Nutritional Stress in Drosophila

et al. 2020

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“…In the abdominal segments, they proliferate while remaining associated with their muscle fibers similar to vertebrate satellite cells [ 207 , 208 ]. During the third larval instar, due to the activation of Wg signaling from the imaginal discs, AMPs undergo asymmetric divisions forming one stem cell and one Numb positive post-mitotic myoblast where Notch signaling is inhibited [ 209 , 210 ]. Thus, a large pool of myoblasts is primed for metamorphosis.…”

Section: Muscle Diversification—on the Road To Muscle Homeostasismentioning

confidence: 99%

Genetic Control of Muscle Diversification and Homeostasis: Insights from Drosophila

Poovathumkadavil

Jagla

2020

Cells

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In the fruit fly, Drosophila melanogaster, the larval somatic muscles or the adult thoracic flight and leg muscles are the major voluntary locomotory organs. They share several developmental and structural similarities with vertebrate skeletal muscles. To ensure appropriate activity levels for their functions such as hatching in the embryo, crawling in the larva, and jumping and flying in adult flies all muscle components need to be maintained in a functionally stable or homeostatic state despite constant strain. This requires that the muscles develop in a coordinated manner with appropriate connections to other cell types they communicate with. Various signaling pathways as well as extrinsic and intrinsic factors are known to play a role during Drosophila muscle development, diversification, and homeostasis. In this review, we discuss genetic control mechanisms of muscle contraction, development, and homeostasis with particular emphasis on the contractile unit of the muscle, the sarcomere.

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“…To ablate myoblasts, we ectopically expressed moleskin (msk) RNAi. Msk is a nuclear importer of the FGF transcriptional activator ERK (Vishal et al, 2017). mskRNAi expression in the myoblasts reduced the number of myoblasts (Fig.…”

Section: Hh Gradients Form Independently In the Wing Disc Asp And Mmentioning

confidence: 94%

Regulated delivery controls Drosophila Hedgehog, Wingless and Decapentaplegic signaling

Hatori

Kornberg

2020

Preprint

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Morphogen signaling proteins disperse across tissues to activate signal transduction in target cells. We investigated dispersion of Hedgehog (Hh), Wingless (Wg), and Bone morphogenic protein homolog Decapentaplegic (Dpp) in the Drosophila wing imaginal disc, and found that delivery to targets is regulated. Cells take up <5% Hh produced, and neither amounts taken up nor extent of signaling changes under conditions of Hh production from 50-200% normal amounts. Similarly, cells take up <25% Wg produced, and variation in Wg production from 50-700% normal has no effect on amounts taken up or signaling. Similar properties were observed for Dpp. Wing disc-produced Hh signals to disc-associated tracheal and myoblast as well as an approximately equal number of disc cells, but the extent of signaling in the disc is unaffected by the presence or absence of the tracheal cells and myoblasts. These findings show that target cells do not take up signaling proteins from a common pool and that both the amount and destination of delivered morphogens are regulated..SummaryThe extent of Hh, Wg, and Dpp signaling is independent of the amount of signal produced or the number of recipient cells.

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Adult Muscle Formation Requires Drosophila Moleskin for Proliferation of Wing Disc-Associated Muscle Precursors

Cited by 8 publications

References 61 publications

Immune Control of Animal Growth in Homeostasis and Nutritional Stress in Drosophila

Immune Control of Animal Growth in Homeostasis and Nutritional Stress in Drosophila

Genetic Control of Muscle Diversification and Homeostasis: Insights from Drosophila

Regulated delivery controls Drosophila Hedgehog, Wingless and Decapentaplegic signaling

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