Nutritional Fortification of Sunflower Meal by Bacillus Subtilis ATCC PTA-6737 Fermentation

Abstract

Sunflower meal with 3 mm particle size (SFM) with 66 or 80% moisture content was inoculated with 1x107 cfu (colony forming unit/g) of Bacillus subtilis ATCC PTA-6737 and fermented for 0, 24, 48 and 72 h. The pH was increased from 5.75 to 9.1 of SFM fermented at both moisture levels with no significant changes in organic acid production. The bacterial growth was peaked at 24 h. Dry matter and crude fibre contents of SFM decreased by 10-13 and 18%, respectively. In contrary, the amount of crude protein, crude ash and soluble amino acid nitrogen increased by 36, 61% and 145%, respectively, with no regard to the effect of moisture content. Phytic acid was degraded up to 42% at both level of moisture content. In conclusion, SFM was enriched with ash and protein and lowered in fibre and phytic acid contents, and can be used as alternative feed material in animal nutrition.

Increased organic acid production and decreased phytate phosphorus by high level of water content of Bacillus subtilis ATCC PTA-6737 fermentation of soybean meal

“…Moreover, the production of organic acids in our study was high in the case of high moisture condition. This result was found contradictory to the results reported by Yasar et al (2018), using the same strain of B. subtilis to ferment sunflower meal. However, Ohara and Yataha (1996) reported lactic acid production by B.…”

“…In these studies, it was demonstrated that the ratio of added water to solid materials in SSF is crucial for improved metabolic activity of microorganism, and directly interacts together with oxygen for the removal of heat generated during the fermentation. In SSF, the water content of fermenting substrate is preferable above 60%, and moreover the optimal level of water content in SBM fermentation studies ranged from 75 to 85% water content (Amadou et al, 2010;Yasar et al, 2018;Karakurt et al, 2019).…”