NUTRITIONAL FACTORS INFLUENCING THE POLYSACCHARIDE PRODUCTION OF
            <i>BRUCELLA SUIS</i>

Gary, N. D.; Kupferberg, L. L.; Anderson, Richard E.; Harein, Phillip K.; Klausmeier, R. E.

doi:10.1128/jb.73.5.632-640.1957

Cited by 8 publications

(6 citation statements)

References 9 publications

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“…Protein determinations were made by the biuret method described by Stickland (25), or by the Folin phenol method of Lowry et al (20). Carbohydrate, after precipitation with alcohol, was measured by the anthrone method outlined by Gary et al (14). Lipid was determined by a modification of the method of Kibrick and Skupp (18).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and Physical Changes in Shaken Suspensions of Pasteurella pestis

Wessman¹,

Miller²

1966

Applied Microbiology

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Pasteurella pestis, harvested after 24 to 30 hr of growth in a casein hydrolysate medium at 26 C, was resuspended and shaken in 3 % lactose-0.1 M phosphate buffer for 4 hr at the same temperature. Certain characteristics of these starved cells were compared with those of control cells. No differences in the amounts of cellular carbohydrate or lipid were detected. The concentrations of the principal free amino acids were greater in the shaken cells, except that they contained no measureable arginine, and the normally large pools of intracellular tricarboxylic acid cycle intermediates were reduced. Greater viable-cell counts resulted with the cells that were shaken in lactose buffer than with the control cells when each was incubated at 5 C for several weeks. However, the reduced viabilities were apparent losses caused by the formation of aggregates of cells. The clumping of cells was caused by the polymerization of extracellular nucleic acids, principally deoxyribonucleic acid, that were excreted by the cells. Cell clumping could be partially prevented by prior shaking of the suspended cells, which removed some of the deleterious material, or by the action of crystalline deoxyribonuclease.

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Section: Methodsmentioning

confidence: 99%

Biochemical and Physical Changes in Shaken Suspensions of Pasteurella pestis

Wessman¹,

Miller²

1966

Applied Microbiology

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“…(With the exception of strain B-4449, the cultures were obtained through the courtesy of M. J. Pickett, University of California at Los Angeles.) Cultures (14 to 18 hr) of these organisms were prepared in the casein acid digest (S-) medium of Gary et al (1957).…”

Section: Methodsmentioning

confidence: 99%

Medium for the Quantitative Recovery of Members of the Genera Pasteurella and Brucella

Cabelli¹,

Levin²

1964

Applied Microbiology

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A modification of peptic digeststarch (PDS) medium for the quantitative enumeration of Pasteurella tularensis is described. This modification (PDM), in which the concentration of L-cysteine was decreased and in which Brilliant Green was substituted for the penicillin added to the original medium, was evaluated and was found satisfactory for the recovery of P. tularensis, P. pestis, and members of the genus Brucella, from freshly harvested and stored cultures as well as aerosolized organisms produced therefrom. With weakly virulent strains of P. tularensis, such as the Jap 4 and LV strains, it was found that PDM was not adequate.

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“…Cells were cultivated, harvested and prepared for the isolation of polysaccharide as follows. Each of a number of Roux bottles, containing 150 ml of agar medium, was inoculated with 2.5 ml of an actively growing, liquid, seed culture (Gary et al, 1957). The agar medium consisted of 0.5 per cent casein partial hydrolyzate, 1 per cent yeast autolysate (yeast-75, Vico Products Co.), 5 per cent glucose (added aseptically prior to solidification of medium), 2 per cent agar and 0.0005 M sodium thioglycolate.…”

Section: Methodsmentioning

confidence: 99%

“…Total carbohydrate content of the dried cells or of the isolated polysaccharide was determined as glucose by the anthrone reaction as previously described (Gary et al, 1957). Reducing sugar content of the polysaccharide was measured according to the procedure of Horvath and Knehr (1941).…”

Section: Methodsmentioning

confidence: 99%

“…It has been demonstrated by Gary et al (1957) that polysaccharide production by Brucella suis can be influenced by specific alterations of the nutritional environment. Their results indicated that the cellular polysaccharide content could be varied significantly by changing the concentrations of casein hydrolyzate or amino acid supplements in the medium, relatively high concentrations reducing the synthesis of polysaccharide.…”

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confidence: 99%

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