Nutritional composition, in vitro antioxidant and anti-diabetic potentials of Breynia retusa (Dennst.) Alston

Rajan, Murugan; Jaganathan, Prabu; Chandran, Rahul; Sajeesh, Thangarajan; Murugaiyan, Iniyavan; Thangaraj, Parimelazhagan

doi:10.1016/j.fshw.2015.12.001

Cited by 17 publications

(13 citation statements)

References 25 publications

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“…Scientists have claimed that highly polar solvents, especially methanol, have a high effectiveness as antioxidants [ 26 ], hence supporting our results. However, our findings are not in line with the study conducted by Murugan, Prabu, Chandran, Sajeesh, Iniyavan and Parimelazhagan [ 18 ], since a different plant part demonstrated high radical scavenging activity. For instance, the methanolic and ethyl acetate leaf extract displayed the highest DPPH and ABTS activity, respectively, (DPPH: IC50 = 2.42 µg/mL; ABTS: 7195.45 ± 44.31 µM TEAC/g).…”

Section: Resultscontrasting

confidence: 99%

“…On the other hand, the dichloromethane fruit extract showed poor antioxidant activity with ABTS, FRAP and CUPRAC tests and inactivity was reported with DPPH and metal chelating assays. Interestingly, despite the fruit showed high nutritional composition in terms of starch, protein, amino acid and mineral content in the work of Murugan, Prabu, Chandran, Sajeesh, Iniyavan and Parimelazhagan [ 18 ], the results presented herein showed that the fruit possessed low phytoantioxidants content.…”

Section: Resultsmentioning

confidence: 59%

“…However, a different trend was observed in another study. The ethyl acetate leaf and fruit extracts were found to be higher in flavonoid content (208.94 ± 50.03 and 259.33 ± 7.37 mg RE/g, respectively) and the methanolic leaf and fruit extracts yielded the highest phenolic content (81.05 ± 0.42 and 53.02 ± 0.95 mg GAE/g, respectively) [ 18 ]. Such difference in results can be explained by a number of parameters including growing conditions, temperature, geographical locations, seasonal variations, nutrients uptake and exposure to pollution [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…The juice prepared from the leaves is used to cure inflammation, hyperglycaemia, diarrhoea, diuretic and relieve body pain. The fruits and twigs are used to treat dysentery and toothache, respectively [ 18 ]. Additionally, the stem can be used to treat conjunctivitis [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the stem can be used to treat conjunctivitis [ 17 ]. Available literatures showed that B. retusa yielded high level of phenolics and possessed antidiabetic and antioxidant effect [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts

et al. 2020

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Breynia retusa (Dennst.) Alston (also known as Cup Saucer plant) is a food plant with wide applications in traditional medicine, particularly in Ayurveda. Extracts obtained with four solvents (dichloromethane, methanol, ethyl acetate and water), from three plant parts, (fruit, leaf and bark) were obtained. Extracts were tested for total phenolic, flavonoid content and antioxidant activities using a battery of assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating. Enzyme inhibitory effects were investigated using acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase and α-glucosidase as target enzymes. Results showed that the methanolic bark extract exhibited significant radical scavenging activity (DPPH: 202.09 ± 0.15; ABTS: 490.12 ± 0.18 mg Trolox equivalent (TE)/g), reducing potential (FRAP: 325.86 ± 4.36: CUPRAC: 661.82 ± 0.40 mg TE/g) and possessed the highest TAC (3.33 ± 0.13 mmol TE/g). The methanolic extracts were subjected to LC-DAD-MSn and NMR analysis. A two-column LC method was developed to separate constituents, allowing to identify and quantify forty-four and fifteen constituents in bark and fruits, respectively. Main compound in bark was epicatechin-3-O-sulphate and isolation of compound was performed to confirm its identity. Bark extract contained catechins, procyanidins, gallic acid derivatives and the sulfur containing spiroketal named breynins. Aerial parts mostly contained flavonoid glycosides. Considering the bioassays, the methanolic bark extract resulted a potent tyrosinase (152.79 ± 0.27 mg kojic acid equivalent/g), α-amylase (0.99 ± 0.01 mmol acarbose equivalent ACAE/g) and α-glucosidase (2.16 ± 0.01 mmol ACAE/g) inhibitor. In conclusion, methanol is able to extract the efficiently the phytoconstituents of B. retusa and the bark is the most valuable source of compounds.

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Section: Resultscontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts

et al. 2020

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Green extraction and biological activity of phenolic extracts from cashew nut testa using a combination of enzyme and ultrasound‐assisted treatments

et al. 2023

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BACKGROUND A combination of enzymes and ultrasound treatment was employed to extract bioactive compounds from cashew nut testa, a by‐product of the food industry. The total catechin, flavonoid, and phenolic content of extracts was investigated together with their biological activity. RESULTS Enzyme and ultrasound‐assisted extraction (E‐UAE) was performed by incubation with Viscozyme L (20 mL kg−1 of testa powder, v/w) for 60 min before sonication for 40 min. Ultrasound and enzyme‐assisted extraction (U‐EAE) was carried out using sonication for 40 min before incubation with Viscozyme L (20 mL kg−1 of testa powder) for 60 min. Under appropriate conditions, the total phenolic, flavonoid, catechin, and epigallocatechin gallate content of the extracts from cashew nut testa obtained from a combination method (U‐EAE or E‐UAE) was significantly higher than that obtained using a single method (EAE or UAE). Extracts of cashew nut testa obtained from E‐UAE displayed significantly higher antioxidant and α‐amylase inhibitory activity than those from the U‐EAE. The E‐UAE extract at a concentration of 100 μg mL−1 had a greater impact on the cell viability of MCF‐7 after treatment (22% cell viability) than did the doxorubicin (DOX) at 4 μg mL−1 (39% cell viability), and the E‐UAE extract at 100 μg mL−1 was considered to be safe for healthy cells because the viability of the bovine aerotic endothelial cells treated with this extract was 91%, which was similar to the DOX treatment. CONCLUSION The extract of cashew nut testa obtained from E‐UAE is valuable and promising for the development of anti‐inflammatory therapeutic drugs. © 2023 Society of Chemical Industry.

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Integrated Genomic and Functional Characterization of the Anti-diabetic Potential of Arthrobacter sp. SW1

et al. 2021

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Nutritional composition, in vitro antioxidant and anti-diabetic potentials of Breynia retusa (Dennst.) Alston

Cited by 17 publications

References 25 publications

Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts

Chromatographic Separation of Breynia retusa (Dennst.) Alston Bark, Fruit and Leaf Constituents from Bioactive Extracts

Green extraction and biological activity of phenolic extracts from cashew nut testa using a combination of enzyme and ultrasound‐assisted treatments

Integrated Genomic and Functional Characterization of the Anti-diabetic Potential of Arthrobacter sp. SW1

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