Tinospora cordifolia is a well-known medicinally important deciduous woody climber plant of Indian subcontinent. The seeds of T. cordifolia are plagued by poor germination capacity due to short duration of viability. Untreated 7-dayold seeds show 83.33% germination. However, a gradual decrease in germination is noticed up to 13.33% within 3 months. This problem raises an obstacle for sustainable use of these seeds. A simple manipulation of germination protocol is standardized to overcome this problem. In this present experiment, gibberellic acid (GA 3 ) and potassium nitrate have been used in different concentrations (100, 300, and 500 ppm) under 16 h/8 h day and night regime at room temperature. The effect of treatment was analyzed by assessing standard germination parameters, seedling growth parameters, vigor index, and biochemical changes in both treated and untreated seeds. The experimental findings suggest that 3-month-old seeds with GA 3 treatment recorded 100% germination. Most of our germination responses display the best result at a higher concentration of GA 3 (300 and 500 ppm) compared to other experimental conditions. Thus, it can be concluded that the GA 3 at 300 ppm has a tremendous promotional effect on the seed germination of T. cordifolia and thus recommended for effective handling of tackling poor seed germinability. extensively spreading climbing shrub found in China and the Indian subcontinent such as Bangladesh, India, and Myanmar Sri Lanka [1,2]. In general, it is found in habitats in deciduous and dry forests. Conventionally, T. cordifolia is widely used as a medicine not only in tribal communities but also in modern countries. In the modern world, medicinally important plants are termed "Green Gold" due to their diverse biological applicability [3,4]. Most importantly, the T. cordifolia plant possess diverse ethnomedicinal values due to its significant application for the treatment of fever, gout, ulcer, rheumatism, diarrhea, old wound, burn, previous findings show that, this plant has anti-inflammatory, anti-allergic, anti-oxidant, antidiabetic, anti-cancer, anti-microbial, immunomodulatory, anti-HIV, and anti-toxic activities [5]. This plant offers a veritable storehouse of biologically important alkaloids, flavonoids, glycosides, and phenolic compounds such as berberine, tinosporine, magnoflorine, cordifolioside, tinocordioside, and isocolumbin. [6].