Nutritional and oxidative status in occupational obese subjects

Abstract

Nutritional status, referred to meat and vegetable food consumption, is related to folate and B 12 vitamin levels; hyperhomocysteinemia (HHcy) is due to folate and B 12 vitamin-methionine metabolism imbalance, which can lead to oxidative stress, OS (imbalance between reactive oxygen species, ROS, and total antioxidant capacity, TAC). Possible early vitamin B 12 (B 12 ) deficiency can be highlighted by holotranscobalamin (HoloTC, bioactive cobalamin fraction) assay. Erythrocyte folate is a biomarker of 2-to 3-month folate storage. To evaluate nutritional status and OS we conducted an observational study on 118 occupational obese subjects (34M/84F, aged 16-69, median 46.5 years; BMI 26.5-54.1, median 33.3 kg/m 2 ) without previous cardiovascular disease. Serum TAC and ROS (spectrophotometry, Diacron International, Italy), serum B 12 and HoloTC, serum and erythrocyte folate, plasma Hcy (immunoenzymatic, AxSYM, Abbott Diagnostics, USA), lipid panel and inflammatory parameters by routine methods. All subjects showed adequate serum and erythrocyte folate levels, but HoloTC values revealed cobalamin deficiency in 30% of cases not congruent with B 12 concentrations, low only in 10%. 43% of cases showed mild HHcy ([10.5 lmol/L; median 12.44 lmol/L, IQR 11.2-16.2). OS was found by normal mean TAC values (370.5 micromolHClO/mL, n.v. [ 350) but increased mean ROS concentrations (386 CarrU, in 93% of subjects. Normal folate and B 12 vitamin levels revealed a good nutritional status in our occupational obese subjects. The presence of Oxidative Stress, due to imbalance between ROS and TAC and mild hyperhomocysteinemia may heighten the obesity-related cardiovascular risk.

Nutrients Intake Is Associated with DNA Methylation of Candidate Inflammatory Genes in a Population of Obese Subjects

“…Dietary nutrition has been widely investigated in an attempt to understand its impact on health and disease status [ 5 , 6 , 7 , 8 ]. Poor diet may be associated with an increased risk of developing chronic diseases via the production of reactive oxygen species (ROS), which may lead to oxidative DNA damage [ 9 , 10 , 11 ].…”

Measurement of Glutathionylated Haemoglobin by MAL-DI-ToF Mass Spectrometry as a Biomarker of Oxidative Stress in Heavy Smokers and in Occupational Obese Subjects

“…One condition which may hamper weight loss notwithstanding interventions on diet and physical exercise is possibly an underlying condition of systemic oxidative stress [3][4][5].…”

Antioxidants in Sport Nutrition

“…Thus, in this specific case, other parameters may be more useful, such as malondialdehyde, which resulted high, and ascorbate, whose levels fell in these patients (Jackson et al 1995). Moreover, much evidence suggests an increased oxidative stress, but the lack of TAC decrease in obese subjects (Vassalle et al 2013a, Puchau et al 2010, Mancini et al 2008, Vigna et al 2010. Thus, the measure of TAC in such subjects might be less important or negligible.…”