NUTRITION OF THE PARASITIC PHASE OF
            <i>COCCIDIOIDES IMMITIS</i>
            IN A CHEMICALLY DEFINED LIQUID MEDIUM

Converse, John L.; Besemer, A. R.

doi:10.1128/jb.78.2.231-239.1959

Cited by 39 publications

(38 citation statements)

References 18 publications

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“…Since the latter was not reproduced consistently in vitro until relatively recently, almost all the information and clinical interpretations from immunological studies were based on tests using coccidioidin: i.e., soluble antigens derived from the saprobic growth cycle (31). The more recent development of cultural methods for reproducible in vitro growth of the parasitic cycle (7)(8)(9)19) made possible the production of spherulin: i.e., soluble antigens derived from endosporulating spherules. Comparison of these two preparations revealed that spherulin was equally specific but more sensitive than coccidioidin as a skin-testing reagent (20,22), but spherulin was considerably less specific than coccidioidin in serological tests (15).…”

mentioning

confidence: 99%

Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis

Huppert¹,

Spratt²,

Vukovich³

et al. 1978

Infect Immun

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MATERLALS AND METHODSAntigen preparations. Coccidioidin was prepared by the toluene lysis method published previously (13, 25) but with the following modification. After lysis of the fungus mat with toluene-saturated distilled water, the filter-sterilized lysate was lyophilized, removing the toluene, and then dissolved with the original broth 541 on August 8, 2020 by guest http://iai.asm.org/ Downloaded from

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mentioning

confidence: 99%

Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis

Huppert¹,

Spratt²,

Vukovich³

et al. 1978

Infect Immun

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“…Germination was eliminated when only a part of the old medium was removed and replenished with C02-bubbled fresh medium. Converse and Besemer (1959) reported that addition of 0.005 mI glutathione increased spherule sizes to 50 to 80 ,. Various concentrations of glutathione were added to our mnedium in an attempt to increase our spherule size, but without success.…”

Section: Discussionmentioning

confidence: 99%

“…Immunological studies by Smith (1960, 1961) with purified spherules as a vaccine source indicated a need for growing mycelium-free spherules. Roessler et al (1946), Levine et al (1960Levine et al ( , 1961, Converse (1955Converse ( , 1956Converse ( , 1957, Converse and Besemer (1959), and Lones and Peacock (1960a, b) reported success in growing the spherules in vitro with the flask-shaking method described by Roessler et al (1946). Spherules and endospores were harvested at the peak of growth by filtering the culture through a fine stainless-steel screen, surgical gauze, and nylon hosiery.…”

mentioning

confidence: 99%

CONTINUOUS IN VITRO CULTIVATION OF SPHERULES OFCOCCIDIOIDES IMMITIS

Breslau

Kubota

1964

J Bacteriol

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Angeles, Calif.), and Mike Y. Kubota. Continuous in vitro cultivation of spherules of Coccidioides immitis. J. Bacteriol. 87:468-472. 1964.-Spherules of Coccidioides immitis were grown in vitro in continuous culture for more than 4 years. Cultures were grown in modified Converse medium in a modified Lubarsky and Plunkett culture tube incubated at 40 C under increased CO2 tension. Spherules that had transformed from hyphae were freed from mycelial fragments, and were allowed to settle to the bottom of the tube. The mycelium which normally degenerated at 40 C was removed repeatedly with a syringe and needle until the culture was almost pure spherules. For continuous culture, 10 to 12 ml of old medium were remnoved, and were replaced by fresh medium previously bubbled with a mixture of 20% carbon dioxide and 80% air.

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“…Increased temperature (between 34 and 40 C) induces spherulation (10,17,18,19,20,46,48,72), but Converse and Besemer (20) stated it is not the only factor influencing development. The studies by Breslau and Kubota (10) also indicated that temperature is a critical factor, but not the only factor for spherulation.…”

Section: Coccidioidesmentioning

confidence: 99%

Nutrition of systemic and subcutaneous pathogenic fungi.

Gilardi¹

1965

Bacteriological Reviews

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NUTRITION OF THE PARASITIC PHASE OF COCCIDIOIDES IMMITIS IN A CHEMICALLY DEFINED LIQUID MEDIUM

Cited by 39 publications

References 18 publications

Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis

Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis

CONTINUOUS IN VITRO CULTIVATION OF SPHERULES OFCOCCIDIOIDES IMMITIS

Nutrition of systemic and subcutaneous pathogenic fungi.

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