MATERLALS AND METHODSAntigen preparations. Coccidioidin was prepared by the toluene lysis method published previously (13, 25) but with the following modification. After lysis of the fungus mat with toluene-saturated distilled water, the filter-sterilized lysate was lyophilized, removing the toluene, and then dissolved with the original broth 541 on August 8, 2020 by guest http://iai.asm.org/ Downloaded from
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