Increasing worldwide competition and shifts in demand, technological advancements, and innovative findings out of research are triggering the ingredient manufacturing industry to include modified processing technologies and novel ingredients. New technologies have been introduced by the ethanol industry to improve the efficiency of ethanol production, resulting in new types of distillers dried grains with different nutrient profiles. One of the new processing techniques removes fibrous corn components before fermentation and removes the soluble fraction after fermentation to produce a high‐protein distillers grain with yeast (HP50Y with 49% protein and HP40Y with 40% protein). The current study was conducted to evaluate the efficacy of HP50Y and HP40Y as a replacement for corn protein concentrate (CPC) in diets of Pacific white shrimp Litopenaeus vannamei. In the first diet series, graded levels of HP50Y (0.0, 5.0, 10.0, 15.0, and 20.0%) were used to replace CPC (13.1, 10.0, 6.6, 3.5, and 0.2%). In the second diet series, graded levels of HP40Y (5.0, 10.0, 15.0, and 20.0%) were used to replace CPC (10.5, 8.0, 5.5, and 2.5%). These diets were evaluated over a 40‐d growth trial (initial weight = 0.54 ± 0.01 g; n = 4). At the conclusion, no significant differences were detected in growth, feed conversion ratio, survival, and food consumption of Pacific white shrimp (P‐value >0.05). However, results from regression analysis revealed that there was a significant increase in percent weight gain of Pacific white shrimp as the percent inclusion level of HP50Y and HP40Y increased in the shrimp diets. Results indicate that HP50Y and HP40Y are both good protein sources and can be used up to a 20% inclusion level in the diets of Pacific white shrimp.