Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit

Abstract

Conjugated linoleic acid was detected in rabbit caecotrophs, due to the presence of microbial lipid activity in rabbit cecum. However, the effect of CLA as a functional food in growing rabbit is not well established. Therefore, this study was conducted to determine the effect of CLA on production, meat quality, and its nutrigenomic effect on edible parts of rabbit carcass including skeletal muscle, liver, and adipose tissue. Therefore, seventy five weaned V-Line male rabbits, 30 days old, were randomly allocated into three dietary treatments receiving either basal control diet, diet supplemented with 0.5% (CLAL), or 1% CLA (CLAH). Total experimental period (63 d) was segmented into 7 days adaptation and 56 days experimental period. Dietary supplementation of CLA did not alter growth performance, however, the fat percentage of longissimus lumborum muscle was decreased, with an increase in protein and polyunsaturated fatty acids (PUFA) percentage. Saturated fatty acids (SFA) and mono unsaturated fatty acids (MUFA) were not increased in CLA treated groups. There was tissue specific sensing of CLA, since subcutaneous adipose tissue gene expression of PPARA was downregulated, however, CPT1A tended to be upregulated in liver of CLAL group only (P = 0.09). In skeletal muscle, FASN and PPARG were upregulated in CLAH group only (P ≤0.01). Marked cytoplasmic vacuolation was noticed in liver of CLAH group without altering hepatocyte structure. Adipocyte size was decreased in CLA fed groups, in a dose dependent manner (P <0.01). Cell proliferation determined by PCNA was lower (P <0.01) in adipose tissue of CLA groups. Our data indicate that dietary supplementation of CLA (c9,t11-CLA and t10,c12- CLA) at a dose of 0.5% in growing rabbit diet produce rabbit meat rich in PUFA and lower fat % without altering growth performance and hepatocyte structure.

Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit

“…Additionally, longitudinal studies of CLA supplementation revealed also different effects of CLA on body weight and body weight gain, some researchers detected body weight re-gain after supplementation of CLA [43,44], and other researchers observed body weight loss [45,46]. To the authors knowledge there are no longitudinal studies that investigated the effect of CLA on growth rate of male rabbits; however, CLA supplementation for short period (49 d) did not affect body weight and feed intake in growing rabbits [27,47]. The contrasting effects of CLA on body weight and feed intake in the above studies could be explained by the difference in species and the period of CLA supplementation.…”

“…Epididymal fatty acids were extracted according to Abdelatty et al, [27], briefly, lipids were extracted with chloroform: methanol mixture (2:1), a standard fatty acid mixture was purchased from Sigma-Aldrich (Sigma, St. Louis, MO). Fatty acids were determined according to O'fallon et al, [28].…”

Effect of poultry offal silage with or without betaine supplementation on growth performance, intestinal morphometry, spleen histomorphology of Nile tilapia (Oreochromis niloticus) fingerlings

“…Biomasses were bulk weighed every week, and the diet was adjusted accordingly. The body weight gain and feed efficiency were calculated according to (Abdelatty et al, 2019; Wanna et al, 2004). Crude protein digestibility was performed according to (El‐Attrouny et al, 2021; Goddard & McLean, 2001).…”

Dietary hydrolyzed soya lecithin affects feed intake, abundance of bacteria in the caecum, fatty acid composition and area of adipocytes in pre‐mating primiparous V‐line female rabbit

“…Rabbits had free access to feed and water (nipple system). Individual feed intake and BW were recorded weekly; daily feed intake, daily BW gain and feed efficiency index (body weight gain/feed intake) were calculated as previously described (Abdelatty et al, 2019).…”