Nutrient transport into germinating Trichoderma atroviride conidia and development of its driving force

Šimkovič, Martin; Olejníková, Petra; Mat'at'a, Matej; Žemla, Peter; Vilimová, Viera; Farkašová, Lenka; Varečka, Ĺudovı́t

doi:10.1099/mic.0.000079

Cited by 3 publications

(1 citation statement)

References 77 publications

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“…The best plant growth responses (height, root length, leaf area, and root, stem and total dry weight) were achieved by inoculating conidia of either T. tomentosum (EMMFRS1C2) or T. harzianum (Mich 6S3C2) in combination with tryptophan 201 ). Expression and activity of H + -ATP ase suggesting that the proton-motive force generated by H + -ATP ase may be an accelerator of nutrient uptake and metabolism (Simkovic et al, 2015). Downregulation of defense genes and upregulation of C and N metabolism genes observed in the microarrays were accompanied by enhanced growth, and increased C and N (Domínguez et al, 2016).…”

Section: Responses To Inoculation With "Elite" Trichoderma Strains Unmentioning

confidence: 99%

Enhancing 15N-uptake in maize (Zea mays L.) by native Trichoderma spp. strains in Central Mexico

Vera-Núñez¹,

Luna-MartÃnez²,

Barcos-Arias³

et al. 2019

Afr. J. Biotechnol.

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A study was undertaken to evaluate the role of Trichoderma strains on 15 N-uptake by maize under greenhouse and field conditions. The Trichoderma strains were isolated from different maize production systems in Guanajuato State, Central Mexico. A total of 39 Trichoderma isolates were obtained, 23 correspond to Trichoderma harzianum (Intra-Genic-Segment). Some colonized the endorhizosphere of maize better than others; however, Trichoderma diversity did not correlate with the maize production system (rainfall vs. irrigation conditions). In greenhouse conditions, biomass production and 15 N-uptake were equally variable; maize plants inoculated with selected Trichoderma species (high root colonization) and fertilized with 140 mg N kg-1 soil, showed similar increase in grain yield and 15 N-uptake vs. those fertilized with 280 mg N kg-1 soil. Biomass and 15 N-uptake directly correlated with the capacity of Trichoderma spp. to colonize the rhizosphere. Under field conditions, the N-fertilizer use efficiency was the highest when maize cv. P30G40 was inoculated with T. harzianum T35 at a N fertilization rate of 180 kg N ha-1 (78%). All measured parameters showed positive effects of inoculation underscoring the feasibility of inoculants with these fungi based on Trichoderma to increase the N-fertilizer use efficiency applied to maize.

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Section: Responses To Inoculation With "Elite" Trichoderma Strains Unmentioning

confidence: 99%

Enhancing 15N-uptake in maize (Zea mays L.) by native Trichoderma spp. strains in Central Mexico

Vera-Núñez¹,

Luna-MartÃnez²,

Barcos-Arias³

et al. 2019

Afr. J. Biotechnol.

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Biotechnological development of Trichoderma-based formulations for biological control

Martínez

Ribera

Schwarze

et al. 2023

Appl Microbiol Biotechnol

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Trichoderma spp. are a genus of well-known fungi that promote healthy growth and modulate different functions in plants, as well as protect against various plant pathogens. The application of Trichoderma and its propagules as a biological control method can therefore help to reduce the use of chemical pesticides and fertilizers in agriculture. This review critically discusses and analyzes groundbreaking innovations over the past few decades of biotechnological approaches to prepare active formulations containing Trichoderma. The use of various carrier substances is covered, emphasizing their effects on enhancing the shelf life, viability, and efficacy of the final product formulation. Furthermore, the use of processing techniques such as freeze drying, fluidized bed drying, and spray drying are highlighted, enabling the development of stable, light-weight formulations. Finally, promising microencapsulation techniques for maximizing the performance of Trichoderma spp. during application processes are discussed, leading to the next-generation of multi-functional biological control formulations. Key points • The development of carrier substances to encapsulate Trichoderma propagules is highlighted. • Advances in biotechnological processes to prepare Trichoderma-containing formulations are critically discussed. • Current challenges and future outlook of Trichoderma-based formulations in the context of biological control are presented.

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Identification of growth stage molecular markers in Trichoderma sp. ‘atroviride type B’ and their potential application in monitoring fungal growth and development in soil

et al. 2015

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Several members of the genus Trichoderma are biocontrol agents of soil-borne fungal plant pathogens. The effectiveness of biocontrol agents depends heavily on how they perform in the complex field environment. Therefore, the ability to monitor and track Trichoderma within the environment is essential to understanding biocontrol efficacy. The objectives of this work were to: (a) identify key genes involved in Trichoderma sp. 'atroviride type B' morphogenesis; (b) develop a robust RNA isolation method from soil; and (c) develop molecular marker assays for characterizing morphogenesis whilst in the soil environment. Four cDNA libraries corresponding to conidia, germination, vegetative growth and conidiogenesis were created, and the genes identified by sequencing. Stage specificity of the different genes was confirmed by either Northern blot or quantitative reverse-transcriptase PCR (qRT-PCR) analysis using RNA from the four stages. con10, a conidial-specific gene, was observed in conidia, as well as one gene also involved in subsequent stages of germination (L-lactate/malate dehydrogenase encoding gene). The germination stage revealed high expression rates of genes involved in amino acid and protein biosynthesis, while in the vegetative-growth stage, genes involved in differentiation, including the mitogen-activated protein kinase kinase similar to Kpp7 from Ustilago maydis and the orthologue to stuA from Aspergillus nidulans, were preferentially expressed. Genes involved in cell-wall synthesis were expressed during conidiogenesis. We standardized total RNA isolation from Trichoderma sp. 'atroviride type B' growing in soil and then examined the expression profiles of selected genes using qRT-PCR. The results suggested that the relative expression patterns were cyclic and not accumulative.

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Nutrient transport into germinating Trichoderma atroviride conidia and development of its driving force

Cited by 3 publications

References 77 publications

Enhancing 15N-uptake in maize (Zea mays L.) by native Trichoderma spp. strains in Central Mexico

Enhancing 15N-uptake in maize (Zea mays L.) by native Trichoderma spp. strains in Central Mexico

Biotechnological development of Trichoderma-based formulations for biological control

Identification of growth stage molecular markers in Trichoderma sp. ‘atroviride type B’ and their potential application in monitoring fungal growth and development in soil

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