Nutrient media optimization for simultaneous enhancement of the laccase and peroxidases production by coculture of Dichomitus squalens and Ceriporiopsis subvermispora

Abstract

Coculturing of two white-rot fungi, Dichomitus squalens and Ceriporiopsis subvermispora, was explored for the optimization of cultivation media for simultaneous augmentation of laccase and peroxidase activities by response surface methodology (RSM). Nutrient parameters chosen from our previous studies with the monocultures of D. squalens and C. subvermispora were used to design the experiments for the cocultivation study. Glucose, arabinose, sodium nitrate, casein, copper sulfate (CuSO4 ), and manganese sulfate (MnSO4 ) were combined according to central composite design and used as the incubation medium for the cocultivation. The interaction of glucose and sodium nitrate resulted in laccase and peroxidase activities of approximately 800 U/g protein. The addition of either glucose or sodium nitrate to the medium also modifies the impact of other nutrients on the ligninolytic activity. Both enzyme activities were cross-regulated by arabinose, casein, CuSO4 , and MnSO4 as a function of concentrations. Based on RSM, the optimum nutrient levels are 1% glucose, 0.1% arabinose, 20 mM sodium nitrate, 0.27% casein, 0.31 mM CuSO4 , and 0.07 mM MnSO4 . Cocultivation resulted in the production of laccase of 1,378 U/g protein and peroxidase of 1,372 U/g protein. Lignin (16.9%) in wheat straw was degraded by the optimized enzyme mixture.

Transcriptome analysis of differential gene expression in Dichomitus squalens during interspecific mycelial interactions and the potential link with laccase induction

“…The copyright holder for this preprint (which was this version posted June 30, 2018. ; https://doi.org/10.1101/359646 doi: bioRxiv preprint When D. squalens (Ds) was co-cultured with T. versicolor (Tv) and P. ostreatus (Po) in Sc medium, the laccases activity was significantly increased in the interaction zones compared to single culture of Ds (Figure 1A), reaching 307.56 U/L in DsTv and 274.50U/L in DsPo, and enhanced changes in laccase activity were as high as 190.04-fold in DsTv and 212.93-fold in DsPo compared to the control. This strong laccase response matched previous findings for Ds during interactions with other species, but the extent of increase in laccase activity was more significant than that in other studies based on the different identity of the competitor and culture conditions (Dong et al, 2011;Kannaiyan et al, 2015). Besides, the result showed laccase activity in the interaction zones of DsTv and DsPo were all higher compared to other two single fungi Tv and Po (Figure 1A).…”

Impact of pH regulation on multicopper oxidase production and swarming motility in the bacterium Proteus hauseri ZMd44

“…In fact, laccase production in some species has been reported to be induced by heat shock or sonication . Besides the surface response methodology , the orthogonal experiments has also been reported to be an effective tool to exclude unnecessary parameters from analysis of laccase production in Escherichia coli as well as for production of other enzymes in fungi . The key factor for the production of Mco‐laccase by P. hauseri ZMd44 was determined to be pH.…”

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

“…In addition to inducement of biosynthesis of new SMs, fungal co-culture has important roles in increasing activity of valuable enzymes (such as laccase, pectinase, etc. [ 97 , 98 , 99 , 100 , 101 ]) and improving yield of important products (such as ursolic acid, oleanolic acid, betulinic acid, etc. [ 91 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 ]).…”