NutriChip: nutrition analysis meets microfluidics

Ramadan, Qasem; Jafarpoorchekab, Hamideh; Huang, Chaobo; Silacci, Paolo; Carrara, Sandro; Köklü, Gözen; Ghaye, Julien Michel; Ramsden, Jeremy J.; Ruffert, Christine; Vergères, Guy; Gijs, Martinus

doi:10.1039/c2lc40845g

Cited by 104 publications

(88 citation statements)

References 38 publications

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“…Higher Lucifer yellow permeability has been described for the same cell type cultured in a microfluidic chamber, but that system did not allow measurement of TEER [30]. Note that Caco-2 cells in a gut-on-a-chip model have reached tighter barrier properties when co-cultured with bacteria and/or immune cells, and exposed to low shear stress and cyclic strain to mimic peristaltic motion [4,32].…”

Section: Intestinal Modelmentioning

confidence: 99%

“…Confluent monolayers of adherent epithelial or endothelial cells grown on culture inserts are widely used static models for intestinal, lung and blood-brain barriers (BBB). The Caco-2 human epithelial cell line is a well characterized model of the intestinal barrier applied for screening of drug candidates [3][4][5][6]. Several studies describe the lung epithelial cell line A549 as a model of the lung barrier [7,8].…”

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confidence: 99%

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A versatile lab-on-a-chip tool for modeling biological barriers

Walter

Valkai

Kincses

et al. 2016

Sensors and Actuators B: Chemical

141

156

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Keywords: Microfluidics Lung and intestinal epithelial cells Endothelial cells Blood-brain barrier PermeabilityTrans-epithelial/endothelial electric resistance a b s t r a c t Models of biological barriers are important to study physiological functions, transport mechanisms, drug delivery and pathologies. However, there are only a few integrated biochips which are able to monitor several of the crucial parameters of cell-culture-based barrier models. The aim of this study was to design and manufacture a simple but versatile device, which allows a complex investigation of barrier functions. The following functions and measurements are enabled simultaneously: co-culture of 2 or 3 types of cells; flow of culture medium; visualization of the entire cell layer by microscopy; real-time transcellular electrical resistance monitoring; permeability measurements. To this end, a poly(dimethylsiloxane)-based biochip with integrated transparent gold electrodes and with a possibility to connect to a peristaltic pump was built. Unlike previous systems, the structure of the device allowed a constant visual observation of cell growth over the whole membrane surface. Morphological characterization of the layers was also accomplished by immunohistochemical staining. The chip was applied to monitor and characterize models of the intestinal and lung epithelial barriers, and the blood-brain barrier. The models were established using human Caco-2 intestinal and A549 lung epithelial cell lines, hCMEC/D3 human brain endothelial cell line and primary rat brain endothelial cells co-cultured with primary astrocytes and brain pericytes. This triple primary co-culture blood-brain barrier model was assembled on a lab-on-a-chip device and investigated under fluid flow for the first time. Such a versatile tool is expected to facilitate the kinetic investigation of various biological barriers.

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Section: Intestinal Modelmentioning

confidence: 99%

mentioning

confidence: 99%

A versatile lab-on-a-chip tool for modeling biological barriers

Walter

Valkai

Kincses

et al. 2016

Sensors and Actuators B: Chemical

141

156

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“…17 Multichamber MPS devices have been developed 121 specifically to study the activation of immune cells by the transfer of nutrients across the gastrointestinal epithelium. 122 The ability to study intestinal flora in MPS has already been demonstrated. 67 But the GI tract is also an entry point for pathogens.…”

Section: Mps Immunologymentioning

confidence: 99%

Fitting tissue chips and microphysiological systems into the grand scheme of medicine, biology, pharmacology, and toxicology

Watson

Hunziker

Wikswo

2017

Exp Biol Med (Maywood)

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Impact statementMicrophysiological systems (MPS), which include engineered organoids and both individual and coupled organs-on-chips and tissue chips, are a rapidly growing topic of research that addresses the known limitations of conventional cellular monoculture on flat plastic -a wellperfected set of techniques that produces reliable, statistically significant results that may not adequately represent human biology and disease. As reviewed in this article and the others in this thematic issue, MPS research has made notable progress in the past three years in both cell sourcing and characterization. As the field matures, currently identified challenges are being addressed, and new ones are being recognized. Building upon investments by the Defense Advanced Research Projects Agency, National Institutes of Health, Food and Drug Administration, Defense Threat Reduction Agency, and Environmental Protection Agency of more than $200 million since 2012 and sizable corporate spending, academic and commercial players in the MPS community are demonstrating their ability to meet the translational challenges required to apply MPS technologies to accelerate drug development and advance toxicology. AbstractMicrophysiological systems (MPS), which include engineered organoids (EOs), single organ/tissue chips (TCs), and multiple organs interconnected to create miniature in vitro models of human physiological systems, are rapidly becoming effective tools for drug development and the mechanistic understanding of tissue physiology and pathophysiology. The second MPS thematic issue of Experimental Biology and Medicine comprises 15 articles by scientists and engineers from the National Institutes of Health, the IQ Consortium, the Food and Drug Administration, and Environmental Protection Agency, an MPS company, and academia. Topics include the progress, challenges, and future of organs-on-chips, dissemination of TCs into Pharma, children's health protection, liver zonation, liver chips and their coupling to interconnected systems, gastrointestinal MPS, maturation of immature cardiomyocytes in a heart-on-a-chip, coculture of multiple cell types in a human skin construct, use of synthetic hydrogels to create EOs that form neural tissue models, the blood-brain barrier-on-a-chip, MPS models of coupled female reproductive organs, coupling MPS devices to create a body-on-a-chip, and the use of a microformulator to recapitulate endocrine circadian rhythms. While MPS hardware has been relatively stable since the last MPS thematic issue, there have been significant advances in cell sourcing, with increased reliance on human-induced pluripotent stem cells, and in characterization of the genetic and functional cell state in MPS bioreactors. There is growing appreciation of the need to minimize perfusate-to-cell-volume ratios and respect physiological scaling of coupled TCs. Questions asked by drug developers are followed by an analysis of the potential value, costs, and needs of Pharma. Of highest value and lowest switching costs may be the d...

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“…Calibration lines and system validation were performed on U937 cell line for both glucose and lactate detection. U937 are hystiocytic lymphoma cells that can differentiate into macrophagelike cells under certain treatments (Ramadan et al, 2013). Many laboratory models investigate the transport of molecules through the epithelial barrier of the gastrointestinal tract.…”

Section: Introductionmentioning

confidence: 99%