Nutmeg's (Myristica Fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties

Rodianawati, Indah; Hastuti, Pudji; Cahyanto, Muhammad Nur

doi:10.1016/j.profoo.2015.01.027

Cited by 37 publications

(28 citation statements)

References 9 publications

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“…White demelanized colonies were observed only in C-9-H-treated and ΔpksP plates ( Figure 3), which may be attributed to the inhibition of DHN-melanin pathway at either pksP/alb1 gene or its gene product PKS protein level. Because demelanization leads to avirulence, 28 further experiments were designed to understand the antimelanogenic associated virulence determinants related to surface organization after C-9-H treatment in A. fumigatus.…”

Section: Resultsmentioning

confidence: 99%

cis-9-Hexadecenal, a Natural Compound Targeting Cell Wall Organization, Critical Growth Factor, and Virulence of Aspergillus fumigatus

et al. 2020

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Aspergillus fumigatus causes several nosocomial pulmonary infections and accounts for high morbidity and mortality rate globally. Among various virulence factors, 1,8-dihydroxynaphthalene-melanin plays an important role in the survival during unfavorable conditions both in vivo and in vitro, masks various molecular patterns associated with A. fumigatus, and protects it from the host immune system. In the present study, we aim to understand the potential of cis-9-hexadecenal as an antimelanogenic compound and its role in modulating other associated virulence factors in A. fumigatus. cis-9-Hexadecenal is a bioactive compound that belongs to C 16 mono-unsaturated fatty-aldehyde groups. Minimum effective concentration of cis-9-hexadecenal affecting A. fumigatus melanin biosynthesis was determined using broth microdilution method. The spectrophotometric analysis revealed reduced melanin content (91%) and hydrophobicity (59%) at 0.293 mM of cis-9-hexadecenal. Cell surface organizational changes using electron microscopy showed altered demelanized smooth A. fumigatus conidial surface without any protrusions after cis-9-hexadecenal treatment. The transcript analysis of polyketide synthase (PKS) pksP/alb1 gene was quantified through qRT-PCR which revealed an upregulated expression. Total proteome profiling conducted through LC-MS-MS showed upregulated PKS enzyme but other downstream proteins involved in the 1,8-dihydroxynaphthalene-melanin biosynthesis pathway were absent. The homology modeling of PKS using Expasy's web server predicted that PKS is stable at varied conditions and is hydrophilic in nature. The Ramachandran plot by PROCHECK confirmed the 3-D structure of PKS to be reliable. Docking analysis using AutoDock-4.2.6 predicted the binding of cis-9-hexadecenal and PKS at Thr-264 and Ser-171 residue via hydrogen bonding at a low binding energy of −4.95 kcal/mol.

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Section: Resultsmentioning

confidence: 99%

cis-9-Hexadecenal, a Natural Compound Targeting Cell Wall Organization, Critical Growth Factor, and Virulence of Aspergillus fumigatus

et al. 2020

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“…It contain oleoresin hydrocarbon monoterpenes 61-88%, that is, α-pinene, βpinene, sabinene monoterpenes acid 5-15%, aromatic ether 2-18% such as myristicin, elemicin, safrole. Kernel contains volatile oil, fats, starch, mucilage, myristicin, myristic acid, while volatile oil contains pinene, sabinene, camphene, elemicin, isoelemicin, eugenol, methoxyeugenol, isoeugenol and safrole (Rodianawati et al, 2015;Sonavane et al, 2002).…”

Section: Myristica Fragrans Houttmentioning

confidence: 99%

Prevention and treatment of different health problems by common peoples diet (Haleem)

Rizwani¹,

Abbas²,

Zahid³

2017

Afr. J. Food Sci.

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“…Nutmeg is known as a spice plant that has economic value and multipurpose because every part of the plant can be utilized in various industries. Besides being used as flavoring spice, nutmeg is also used as a medicinal material because it has properties as antioxidants, antimicrobial [2] and antifungals [3]. Nutmeg plant is one of the essential oil producing plants that known as nutmeg oil [4].…”

Section: Introductionmentioning

confidence: 99%

ISOLATION OF ESSENSIAL OIL OF NUTMEG (Myristica fragrans Houtt) and ANTIOXIDANT ACTIVITY TEST WITH DPPH

Ginting

Maira

Mustanir

et al. 2018

J. Nat.

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Abstract. The essential oil of the nutmeg plant (Myristica fragrans Houtt) has been obtained from roots, bark, fruit, mace and seeds using Stahl steam distillation. Each essential oil have tested for antioxidant activity with DPPH. Antioxidant activity of essential oil from each nutmeg plant to DPPH with concentration 25, 50 and 100 ppm. Each essential oil did not show strong antioxidant activity but the result of nutmeg isolation had strong antioxidant activity with IC 50 that was 80,555 ppm. Based on GC-MS analysis of essential oil of nutmeg isolate obtained myristicin compound with 96.52% area and time Retention 22.127. Myristicin is one of the main components of essential oils of nutmeg plants that play an important role as antioxidants.

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Nutmeg's (Myristica Fragrans Houtt) Oleoresin: Effect of Heating to Chemical Compositions and Antifungal Properties

Cited by 37 publications

References 9 publications

cis-9-Hexadecenal, a Natural Compound Targeting Cell Wall Organization, Critical Growth Factor, and Virulence of Aspergillus fumigatus

cis-9-Hexadecenal, a Natural Compound Targeting Cell Wall Organization, Critical Growth Factor, and Virulence of Aspergillus fumigatus

Prevention and treatment of different health problems by common peoples diet (Haleem)

ISOLATION OF ESSENSIAL OIL OF NUTMEG (Myristica fragrans Houtt) and ANTIOXIDANT ACTIVITY TEST WITH DPPH

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