A performance of heat transfer fluids has a substantial influence on the size, weight and cost of heat transfer systems, therefore, a high-performance heat transfer fluid is very important in many industries. Over the last decades, nanofluids have been developed. According to many researchers and publications on nanofluids, it is evident that nanofluids have a high thermal conductivity. The aim of this experimental study was to investigate the change of the workpiece temperature during drilling using Jatropha oil with iron nanoparticles and water with iron nanoparticles as lubricating and cooling fluids. These experiments were carried out with samples of nanofluid with different nanoparticles volume ratio, such as samples JN 1, JN 5 and JN 10 of iron nanoparticles in the base Jatropha oil with a nanoparticle volume fraction of 1 %, 5 % and 10 % respectively and samples W N 1, W N 5 and W N 10 of iron nanoparticles in the base water with a nanoparticle volume fraction of 1 %, 5 % and 10 % respectively.