In this study, the authors numerically investigate the frequency response on the three-dimensional thermal convection in a cubic cavity heated below in the gravitational field, concerning flow characteristics such as flow structure and a global quantity the spatially-averaged kinetic energy . The authors assume incompressible fluid with a Prandtl number Pr = 7.1 (water) and with a Rayleigh number Ra = 1.0×10 4 or 4. can observe the optimum frequency where the amplitude of attains the maximum, each η. And, for both Ra's, becomes the minimum at η = 1.5 -2.0. Especially for Ra = 4.0×10 4 , is affected by the initial conditions. For both Ra's, the maximum of the amplitude uniquely exists at each η, when η < 1.5. On the other hand, we can observe not single but plural peak frequencies with locally-maximum 's each η, when η ≥ 1.5. It is confirmed that such plural frequencies are related with the appearances of various flow structures such as S1, S2, S4, S5, S6 and S8. Especially for Ra = 4.0×10 4 , this relation is also affected by the initial conditions. In addition, the details of a new flow structure S8 is reported.