Numerical investigation into the effects of ordered particle packing and slip flow on the performance of chromatography

Abstract

The pressure drop and the plate height of chromatography columns packed with particles in the face-centered cubic, the body-centered cubic and the simple cubic configurations are calculated by a volume averaging method model. It is found that the Kozeny-Carman equation provides a reasonable prediction of the pressure drop when particles are in the face-centered cubic configuration, but overestimates the pressure drop when particles are in the body-centered cubic and the simple cubic configurations. The face-centered cubic configuration has the advantage to provide a smaller longitudinal dispersion coefficient than the body-centered cubic, the simple cubic, and the random configurations. The pressure drop and the plate height for slip flow through particles in the face-centered cubic configuration are lower than that for no-slip flow. The values of the smallest reduced plate height of columns packed with particles in the face-centered cubic configuration for no-slip flow and slip flow are about 0.084 and 0.059, respectively. The plate height of the ordered particle packing structures is smaller and the effect of slip flow on the plate height is less remarkable than results reported in literature.

Core–Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography

“…For the case of the protein bovine serum albumen, this ratio is 1.5 %. They concluded with agreement with the results of Yan and Wang 77 that extraordinary values of h are possible even without slip flow, that flow rate is enhanced by slip flow and that use of particle diameters tailored to the analyte diameter might give a lower h by excluding analyte from stagnant regions.…”

Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations

“…However, simulations of slip flow in packed beds with colloidal silica particles predicted a much reduced flow enhancement than the experimental results described above [166, 167]. While the theory underlying slip flow in open capillaries is understood, the theory behind slip flow in packed beds has recently been investigated.…”

“…As such the discrepancy between the experimental and simulated results is not definitively understood as yet. That said, simulations of flow moving through the packed bed indicated that regions of stagnant flow exist where the fluid stream makes contact with the particles indicating that tortuosity may affect the flow velocity profile in slip flow chromatography thereby reducing the flow enhancement [166, 167]. …”

Predictive model of solute transport with reversible adsorption in spatially periodic hierarchical porous media

“…Most theoretical and experimental works addressing solute transport in porous media have focused on porous media comprising impermeable particles, pillars or monoliths [4,[13][14][15][16][17][18][19][20]. Perhaps due to their complex pore geometry, experimental and theoretical predictions of solute transport in hierarchical porous media are few.…”