Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Shamloo, Amir; Kamali, Ali

doi:10.1002/jssc.201700325

Cited by 50 publications

(21 citation statements)

References 27 publications

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“…also demonstrated the high separation efficiency between 13 μm and 5 μm beads with nearly 100% separation [32]. However, all these devices only reported investigations with particles of vastly different sizes or vastly different dielectric properties resulting in major shifts in Clausius–Mossotti factors [1,10,30,32,33]. Similarly, Zhou et al.…”

Section: Resultsmentioning

confidence: 99%

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

Kikkeri

Kerr

Bertke

et al. 2020

J of Separation Science

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Rapid and accurate purification of various heterogeneous mixtures is a critical step for a multitude of molecular, chemical, and biological applications. Dielectrophoresis has shown to be a promising technique for particle separation due to its exploitation of the intrinsic electrical properties, simple fabrication, and low cost. Here, we present a geometrically novel dielectrophoretic channel design which utilizes an array of localized electric fields to separate a variety of unique particle mixtures into distinct populations. This label-free device incorporates multiple winding rows with several nonuniform structures on to sidewalls to produce high electric field gradients, enabling high locally generated dielectrophoretic forces. A balance between dielectrophoretic forces and Stokes' drag is used to effectively isolate each particle population. Mixtures of polystyrene beads (500 nm and 2 μm), breast cancer cells spiked in whole blood, and for the first time, neuron and satellite glial cells were used to study the separation capabilities of the design. We found that our device was able to rapidly separate unique particle populations with over 90% separation yields for each investigated mixture. The unique architecture of the device uses passivated-electrode insulator-based dielectrophoresis in an innovative microfluidic device to separate a variety of heterogeneous mixture without particle saturation in the channel. K E Y W O R D Sbreast cancer, circulating tumor cells, dielectrophoresis, neuron cells, satellite glial cells 1576

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Section: Resultsmentioning

confidence: 99%

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

Kikkeri

Kerr

Bertke

et al. 2020

J of Separation Science

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show abstract

“…A particle in a ow is a ected by the uid velocity, which can be described by the hydrodynamic drag force. For a spherical particle, the hydrodynamic drag force is de ned as follows [29,36]:…”

Section: Hydrodynamic Friction (Stokes Drag Force)mentioning

confidence: 99%

“…The direction of DEP force and hydrodynamic drag force depends on the electric eld and ow streamlines, respectively. The velocity, which is induced on the particle, can be computed by a balance between DEP force and hydrodynamic drag force [29,36]:…”

Section: Hydrodynamic Friction (Stokes Drag Force)mentioning

confidence: 99%

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

2019

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Insulator-based dielectrophoresis is a recently developed technique in which insulating posts are used to produce non-uniformity in the electric eld in a microchannel. This study presents the e ects of insulating posts' geometry and arrangement on the trapping e ciency of red blood cells in an alternating current-insulator-based dielectrophoresis system. Microchannels containing square, circular, and diamond-shaped posts with particles under the in uence of positive dielectrophoresis force and uid ow were considered in this study. The nite element method was used to compute the velocity of the ow and electric eld. The numerical method was veri ed by comparing the numerical results with experimental data. Two distinct criteria for examining particle trapping for distinct shapes and arrangements of insulating posts were introduced. Particle tracing simulation was implemented to observe particle trapping and compare the trapping performance of systems with distinct posts. As shown in the results for the system with circular and square posts, insulators should be narrowed to improve particle trapping, while the diamond post should be widened to increase the trapping e ciency. In addition, the particle tracking results showed that the microchannel with square posts was more e cient in particle trapping.

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“…DEP has been demonstrated to be capable of separating cells based on the variations in the dielectric properties of different cell types. However this method is relatively slow, that affects the throughput of cell separation Li 1997, Shamloo andKamali 2017).…”

Section: Active Cell Separation Techniquesmentioning

confidence: 99%

Altered basophil function in patients with chronic kidney disease on hemodialysis

Aljadi¹,

Nopp²,

Winqvist³

et al. 2017

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Numerical analysis of a dielectrophoresis field‐flow fractionation device for the separation of multiple cell types

Cited by 50 publications

References 27 publications

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

Passivated‐electrode insulator‐based dielectrophoretic separation of heterogeneous cell mixtures

Numerical study of insulation structure characteristics and arrangement effects on cell trapping using alternative current insulating based dielectrophoresis

Altered basophil function in patients with chronic kidney disease on hemodialysis

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