Altered basophil function in patients with chronic kidney disease on hemodialysis

Abstract

Together, these data demonstrate that basophil functions related to adhesion and degranulation are altered in CKD patients on hemodialysis, which indicates a potential role for the basophil in the pathogenesis of complications related to infections.

Adhesion molecule cross‐linking and cytokine exposure modulate IgE‐ and non‐IgE‐dependent basophil activation

“…24 CD62L is of interest for its role in basophil adhesion and its unique stable expression on basophils. 13 Indeed, cross-linking of CD203c and that of the adhesion molecules CD62L, CD11b and CD49d show a significant basophil activation judged by %CD63 expression. These experiments were performed at different temperatures (4°o r RT) to investigate the possibility of a temperature-dependent effect.…”

“…CD62L has been found to be quite stable on the basophil surface regardless of stimuli. 13 The observation that IL-33 has a more pronounced effect on CD62L shedding than IL-3 and fMLP is of special interest as IL-33 is secreted by endothelial cells 16 and mast cells 25 and is involved in the recruitment of basophils into the inflammatory foci. Considering that IL-33 is mainly found in tissues, we speculate that IL-33-dependent CD62L shedding is more likely to occur at extravascular sites.…”

“…The adhesion process involves L‐selectin (CD62L), CR3 (CD11b, CD18) and VLA‐4 (CD49d, CD29), where fibronectin is a ligand for both CR3 and VLA‐4 12 . CD62L has previously been shown to have a stable expression on basophils upon activation, 13 in contrast to neutrophils and monocytes, but its function is not yet fully established. All three adhesion markers are involved in different steps in basophil rolling, adhesion and transmigration in vivo, 14 and these events are applicable also in the microfluidic chip.…”

A novel tool for clinical diagnosis of allergy operating a microfluidic immunoaffinity basophil activation test technique

“…L-selectin (CD62L) and very late antigen 4 (VLA-4, CD49d/CD29) are involved during basophil tethering and rolling on the endothelium. CD62L is a selectin that is not downregulated on basophils in the same way as it is on neutrophils upon activation [60]. The CD62L shedding on leukocytes is regulated by the cytoplasmic tail but also by mechanical shedding during rolling [61].…”

“…IL-33 induced CD62L shedding might tune the CD62L binding to ligands in tissue, such as proteoglycans [61]. It has been reported that CD62L is quite stable on basophils upon stimulation via several pathways such as TLR2, TLR4 and fMLP but that anti-FcεRI activation significantly downregulates CD62L [60]. Anaphylactic degranulation of basophils instantly releases histamine and cytokines such as IL-4 as well as expresses CD63 on the cell surface in response to activation.…”