Number of microvesicles in peripheral blood and ability of plasma to induce adhesion between phospholipid membranes in 19 patients with gastrointestinal diseases

Janša, Rado; Šuštar, Vid; Frank, Mojca; Sušanj, Petra; Bešter, J.; Manček-Keber, Mateja; Kržan, Mojca; Iglič, Aleš

doi:10.1016/j.bcmd.2008.01.009

Cited by 26 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma contains proteins that mediate attractive interaction between membranes [47-50]. EPs may enhance the mediating effect of proteins by forming protein-EP complexes with extended charge distribution and exposure of positively charged domains that favour interaction with the negatively charged glycolipid coat of the erythrocyte membrane.…”

Section: Discussionmentioning

confidence: 99%

Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

et al. 2013

Self Cite

View full text Add to dashboard Cite

BackgroundMassive industrial production of engineered nanoparticles poses questions about health risks to living beings. In order to understand the underlying mechanisms, we studied the effects of TiO2 and ZnO agglomerated engineered nanoparticles (EPs) on erythrocytes, platelet-rich plasma and on suspensions of giant unilamelar phospholipid vesicles.ResultsWashed erythrocytes, platelet-rich plasma and suspensions of giant unilamelar phospholipid vesicles were incubated with samples of EPs. These samples were observed by different microscopic techniques. We found that TiO2 and ZnO EPs adhered to the membrane of washed human and canine erythrocytes. TiO2 and ZnO EPs induced coalescence of human erythrocytes. Addition of TiO2 and ZnO EPs to platelet-rich plasma caused activation of human platelets after 24 hours and 3 hours, respectively, while in canine erythrocytes, activation of platelets due to ZnO EPs occurred already after 1 hour. To assess the effect of EPs on a representative sample of giant unilamelar phospholipid vesicles, analysis of the recorded populations was improved by applying the principles of statistical physics. TiO2 EPs did not induce any notable effect on giant unilamelar phospholipid vesicles within 50 minutes of incubation, while ZnO EPs induced a decrease in the number of giant unilamelar phospholipid vesicles that was statistically significant (p < 0,001) already after 20 minutes of incubation.ConclusionsThese results indicate that TiO2 and ZnO EPs cause erythrocyte aggregation and could be potentially prothrombogenic, while ZnO could also cause membrane rupture.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, the concentration of nanovesicles was found to be increased in patients with lung cancer, 74 dermatofibroma, 87 dermatofibrosarcoma protuberans, 87 carcinoma of the oral cavity, 88 ovarian cancer, 89 and gastrointestinal cancer. 90,91 It was recently suggested that the material isolated from blood contains both nanovesicles and residual cells, and that residual cells, mostly platelets, are the origin of the nanovesicles found in isolates as an artifact of the isolation procedure. 64 However, clinical studies show differences between concentrations of nanovesicles isolated from the blood of patients with cancer and from that from healthy 54.191.190.102 on 12-May-2018 For personal use only.…”

Section: Clinical Relevance Of Cell Nanovesiclesmentioning

confidence: 99%

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Kralj‐Iglič¹

2012

IJN

View full text Add to dashboard Cite

Abstract:Membranous nanostructures, such as nanovesicles and nanotubules, are an important pool of biological membranes. Recent results indicate that they constitute cell-cell communication systems and that cancer development is influenced by these systems. Nanovesicles that are pinched off from cancer cells can move within the circulation and interact with distant cells. It has been suggested and indicated by experimental evidence that nanovesicles can induce metastases from the primary tumor in this way. Therefore, it is of importance to understand better the mechanisms of membrane budding and vesiculation. Here, a theoretical description is presented concerning consistently related lateral membrane composition, orientational ordering of membrane constituents, and a stable shape of nanovesicles and nanotubules. It is shown that the character of stable nanostructures reflects the composition of the membrane and the intrinsic shape of its constituents. An extension of the fluid mosaic model of biological membranes is suggested by taking into account curvature-mediated orientational ordering of the membrane constituents on strongly anisotropically curved regions. Based on experimental data for artificial membranes, a possible antimetastatic effect of plasma constituents via mediation of attractive interaction between membranous structures is suggested. This mediated attractive interaction hypothetically suppresses nanovesiculation by causing adhesion of buds to the mother membrane and preventing them from being pinched off from the membrane.

show abstract

“…[35][36][37][38][39] The results of numerous clinical studies showed that the concentration of nanoparticles in isolates is increased in patients with a variety of diseases compared with healthy controls. 30,40,41 However, a method based on the isolation and assessment of nanoparticles from blood that would be of satisfactory repeatability and accuracy and therefore suitable for diagnosis and treatment in clinical practice has not yet been established. Also the mechanisms responsible for budding and vesiculation, including the processes that occur during the isolation procedure, are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

Šuštar¹,

Bedina-Zavec²,

Stukelj³

et al. 2011

IJN

View full text Add to dashboard Cite

Background: Shedding of nanoparticles from the cell membrane is a common process in all cells. These nanoparticles are present in body fluids and can be harvested by isolation. To collect circulating nanoparticles from blood, a standard procedure consisting of repeated centrifugation and washing is applied to the blood samples. Nanoparticles can also be shed from blood cells during the isolation process, so it is unclear whether nanoparticles found in the isolated material are present in blood at sampling or if are they created from the blood cells during the isolation process. We addressed this question by determination of the morphology and identity of nanoparticles harvested from blood. Methods: The isolates were visualized by scanning electron microscopy, analyzed by flow cytometry, and nanoparticle shapes were determined theoretically. Results: The average size of nanoparticles was about 300 nm, and numerous residual blood cells were found in the isolates. The shapes of nanoparticles corresponded to the theoretical shapes obtained by minimization of the membrane free energy, indicating that these nanoparticles can be identified as vesicles. The concentration and size of nanoparticles in blood isolates was sensitive to the temperature during isolation. We demonstrated that at lower temperatures, the nanoparticle concentration was higher, while the nanoparticles were on average smaller. Conclusion:These results indicate that a large pool of nanoparticles is produced after blood sampling. The shapes of deformed blood cells found in the isolates indicate how fragmentation of blood cells may take place. The results show that the contents of isolates reflect the properties of blood cells and their interaction with the surrounding solution (rather than representing only nanoparticles present in blood at sampling) which differ in different diseases and may therefore present a relevant clinical parameter.

show abstract

Number of microvesicles in peripheral blood and ability of plasma to induce adhesion between phospholipid membranes in 19 patients with gastrointestinal diseases

Cited by 26 publications

References 31 publications

Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

Contact Info

Product

Resources

About