Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

Šimundić, Metka; Drašler, Barbara; Šuštar, Vid; Zupanc, Jernej; Štukelj, Roman; Makovec, Darko; Erdoğmuş, Deni̇z; Hägerstrand, Henry; Drobne, Damjana; Kralj‐Iglič, Veronika

doi:10.1186/1746-6148-9-7

Cited by 35 publications

(38 citation statements)

References 53 publications

(50 reference statements)

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“…It seems evident that the binding of TiO 2 − with the membrane takes place through relatively weak contacts since NP did not immerse deeply in the membrane but floated on top of the bilayer surface, enabling diffusion of the membrane, with no adsorption to the membrane. This is in line with other study, where reported that a noticeable and statistically significant (p<0,001) influence on phospholipid vesicles were observed after incubation of ZnO (but not TiO 2 ) nanoparticles [33]. This is known that agglomerates larger than 0,2 μm were found attached to the membrane [34], whereas smaller aggregates and single particles could probably cross the membrane through pores or ion channels [35].…”

Section: Zno and Tio 2 Np's Interaction With Slbsupporting

confidence: 80%

“…As this process could distort the membrane structure, characterization tools that enable us to study the structure (ordering) of the membrane also provide information about the influence of NPs on the membranes. ZnO and TiO 2 nanoparticles and their interaction with biological objects were investigated by several authors using different methods [6][7][8][9][10], however the mechanism of NP interaction with membrane is still an open question. Supercritical Angle Fluorescence Microscopy (SAF) is a technique that allows detection and characterisation of fluorescent species (proteins, biomolecules, pharmaceuticals, etc.)…”

Section: Introductionmentioning

confidence: 99%

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Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Grinceviciute¹,

Verdes²,

Snitka³

2015

JNMR

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Nanoparticles (NP) are broadly exploited in biomedical sciences in order to develop various methods of targeted drug delivery, novel biosensors and new therapeutic pathways. However, relatively little is known in the negotiation of NPs with complex biological environments. NP interaction with cell membranes can damage the cell membrane and cause toxicity; therefore, examining interactions between NPs and cell membranes is crucial to understanding NP toxicity mechanisms and the development of safe and non-toxic NP-based commercial and therapeutic applications. To gain a physical understanding of NP-membrane interactions, we used a simplified system built of well-defined synthetic lipid bilayers and NP. The interaction of nanoparticles (NPs) with supported lipid bilayers (SLB) can lead to structural modification of the SLB and affect the structure and dynamic of lipids. In this work TiO 2 and ZnO nanoparticles were chosen because of wide applications and usage in industry of papers, inks, medicines, food products, cosmetics, toothpastes and skin care products and among others. Therefore, a better understanding of the interactions between NP and lipid membrane may help to better clarify the potential risk of NPs. The interaction between ZnO and TiO 2 NPs and lipid membranes was studied by using scanning supercritical angle fluorescence microscopy. The biological response to elucidated changes in lipid membrane structure/characteristics under ZnO or TiO 2 NP influence was tested by fluorescence correlation spectroscopy. It was found the significant reduction of lipids diffusion mobility, which can be explained as a result of lipid-ZnO aggregates binding, depending on ZnO concentration. The TiO 2 has a little effect on lipids diffusion mobility.

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Section: Zno and Tio 2 Np's Interaction With Slbsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Grinceviciute¹,

Verdes²,

Snitka³

2015

JNMR

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“…32,33 It is known that adsorption of NPs onto the surface of RBCs can change cell morphology and erythrocyte sedimentation rate, agglutination of RBCs, and hemolysis. 1,18,27,[34][35][36][37][38][39] An interesting question is whether mechanisms revealed by experimental studies of artificial membranes or computer simulations of interactions between NPs and artificial lipid membranes can also explain the interactions of NPs with biological membranes.…”

Section: Introductionmentioning

confidence: 99%

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Drašler¹,

Drobne²,

Novak³

et al. 2014

IJN

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Background The purpose of this work is to provide experimental evidence on the interactions of suspended nanoparticles with artificial or biological membranes and to assess the possibility of suspended nanoparticles interacting with the lipid component of biological membranes. Methods 1-Palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine (POPC) lipid vesicles and human red blood cells were incubated in suspensions of magnetic bare cobalt ferrite (CoFe 2 O 4 ) or citric acid (CA)-adsorbed CoFe 2 O 4 nanoparticles dispersed in phosphate-buffered saline and glucose solution. The stability of POPC giant unilamellar vesicles after incubation in the tested nanoparticle suspensions was assessed by phase-contrast light microscopy and analyzed with computer-aided imaging. Structural changes in the POPC multilamellar vesicles were assessed by small angle X-ray scattering, and the shape transformation of red blood cells after incubation in tested suspensions of nanoparticles was observed using scanning electron microscopy and sedimentation, agglutination, and hemolysis assays. Results Artificial lipid membranes were disturbed more by CA-adsorbed CoFe 2 O 4 nanoparticle suspensions than by bare CoFe 2 O 4 nanoparticle suspensions. CA-adsorbed CoFe 2 O 4 -CA nanoparticles caused more significant shape transformation in red blood cells than bare CoFe 2 O 4 nanoparticles. Conclusion Consistent with their smaller sized agglomerates, CA-adsorbed CoFe 2 O 4 nanoparticles demonstrate more pronounced effects on artificial and biological membranes. Larger agglomerates of nanoparticles were confirmed to be reactive against lipid membranes and thus not acceptable for use with red blood cells. This finding is significant with respect to the efficient and safe application of nanoparticles as medicinal agents.

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“…The NPs-induced RBC aggregation was lower with negatively charged NPs [11]. Similarly, Simundic et al reported on the induction of RBC aggregation by TiO 2 and ZnO NPs [12]. In addition, Smyth et al, have shown the induction of platelets aggregation by PS-NPs [13].…”

Section: Introductionmentioning

confidence: 91%

“…Numerous studies have examined NP-RBC interaction, focusing on the penetration of NPs into cells, [6,7] and the hemolytic potential of NPs [8][9][10], while only a few studies investigated the effect of NPs on cell-cell interaction [11,12]. In these studies, the effects of the size and surface charge of the hydroxyapatite NPs on the RBCs morphology and aggregation were investigated, showing that in protein-free medium, where no aggregation occurs, NPs induced RBC aggregation [11].…”

Section: Introductionmentioning

confidence: 99%

Polystyrene Nanoparticles Activate Erythrocyte Aggregation and Adhesion to Endothelial Cells

Barshtein

Livshits

Shvartsman

et al. 2015

Cell Biochem Biophys

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Nanoparticles (NPs) are drawing an increasing clinical interest because of their potential use as drug carriers. Recently, a new strategy for elevation of NPs in vivo circulation time has been proposed, specifically, utilizing red blood cells (RBCs) as a carrier for NPs, that are loaded with a drug, by interaction (in vitro) of human RBCs with NPs (RBCNP). This class of delivery set-up, combines advantages of natural RBCs and synthetic biomaterials. Previous studies demonstrated that NPs initiated hemolysis of RBC and activated cells aggregation. In the present study, we examined the effect of RBCNP on the aggregation of RBC and their adhesion to endothelial cells (EC). Red cells were treated with polystyrene NPs (PS-NP), and following their washing, were added to suspension of untreated cells at various concentrations. We observed that the PS-NP and RBCNP initiated the formation of red cells aggregates and markedly elevated RBC adhesion to EC. These effects were augmented with (a) increasing concentration of NPs or RBCNP, and (b) with decreasing NP size. This implies that RBCNP are cells with a stronger intercellular interaction, and may thereby induce the formation of large and strong aggregates with untreated RBC, as well as strong RBC/EC interaction.

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Effect of engineered TiO2and ZnO nanoparticles on erythrocytes, platelet-rich plasma and giant unilamelar phospholipid vesicles

Cited by 35 publications

References 53 publications

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Effect of Zinc Oxide and Titanium Dioxide Nanoparticles on Supported Lipid Bilayers

Effects of magnetic cobalt ferrite nanoparticles on biological and artificial lipid membranes

Polystyrene Nanoparticles Activate Erythrocyte Aggregation and Adhesion to Endothelial Cells

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