Number of blastomeres and chromosome abnormalities of bovine blastocysts obtained from in vitro matured, fertilized and cultured follicular oocytes.

Required Concentration and Time of Vinblastine Treatment for Chromosome Preparation in Bovine Blastocysts Derived from In Vitro Fertilization.

“…The incidence of chromosomal aberrations in the present study (15.6%), was lower than those in previous reports [5,7,8], since the sperm concentration used in the present study was lower than those in the other reports. Kajihara et al [5] have demonstrated chromosome abnormality in bovine blastocysts derived from in-vitro fertilization to be 22%, composed of triploids and hyperdiploids.…”

“…The tendency of male advantage in diploid embryos compared with those found by some other researchers [17,20], although Kajihara et al [5] and Tokumaru et al [16] found a tendency toward This embryo was treated with vinblastine (100 ng/ ml) for 10 h. female advantage.…”

“…In investigations of chromosomes, with various mitotic inhibitors, many researchers have used short treatment times for bovine embryos fertilized in vitro. King et al [15] have described a method for preparing chromosomes from bovine blastocysts using 200 µg/ ml colchicine for 2 to 9.5 h. They have shown the mitotic indexes to be 14.7-37.5% in morphologically good blastocysts treated for 8.5-9.5 h, and 4.2-14.3% in embryos treated for 2-4 h. Kajihara et al [5] have reported frequencies of bovine embryos having metaphase plates of approximately 80-83%, and the sexing rate of 63.4% in embryos treated with 0.04 µg/ml colcemid for 1.5-3 h. Tokumaru et al [16] have also reported mitotic indexes of about 5-7% in bovine blastocysts treated with 0.04 µg/ml colcemid for 2 h. Avery et al [17] have shown a low mitotic index of 3.5%, in bovine in-vitro fertilized blastocysts treated with 0.01 µg/ml colcemid for 2 h. Iwasaki et al have reported mitotic indexes of approximately 5 to 12% in bovine blastocysts treated for 4 h with a combination of vinblastine sulfate and podophyllotoxin [7], and a mitoic index of approximately 26% in morulae or blastocysts treated for 6 h with 0.4 µg/ ml vinblastine sulfate [8]. If the treatment time is longer, the mitotic index is higher.…”

“…Kajihara et al [5] have demonstrated chromosome abnormality in bovine blastocysts derived from in-vitro fertilization to be 22%, composed of triploids and hyperdiploids. Iwasaki et al [7] have reported the incidence of chromosomal anomalies in bovine blastocysts derived from in-vitro fertilization to be 37.5%, showing high triploidy and mixoploidy.…”

“…However, we believe that the efficient conditions for chromosome preparation in mouse embryos are not applicable to that in bovine embryos, because the conditions of cultivation in vitro of bovine embryos differs from those of mouse embryos. In the present study, we determined the effective concentration and treatment time of vinblastine sulfate to obtain the greatest number of metaphase plates, and the highest mitotic indexes in chromosome any cytogenetic studies for chromosomal anomalies and sex ratios in bovine embryos M fertilized in vivo [1][2][3] or in vitro [4][5][6][7][8] have been reported to date because of the importance of chromosomal anomalies as an adverse factor after transfer to recipients [9]. For the cytogenetic studies of bovine embryos, it is absolutely necessary to accumulate substantial numbers of analyzable metaphase plates.…”