Effect of the number of granulosa cells added to culture medium for in vitro maturation, fertilization and development of bovine oocytes

Mochizuki, Hitoshi; Fukui, Yutaka; Ono, Hitoshi

doi:10.1016/0093-691x(91)90322-5

Cited by 43 publications

(11 citation statements)

References 29 publications

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“…Significantly more cumulus-enclosed oocytes were fertilized and then developed to the blastocyst stage in vitro when compared with cumulus-free oocytes (Shioya et al, 1988;Mochizuki et al, 1991). A high proportion of abnormal fertilization was found when cumulus-free oocytes were used for in vitro fertilization (Vanderhyden and Armstrong, 1989).…”

Section: Introductionmentioning

confidence: 98%

“…Numerous reports on oocyte in vitro maturation and fertilization (IVM/IVF) have suggested that the oocyte appears to acquire developmental competence only when the cumulus cells are directly contacting the oocyte (Leibfried-Rutledge et al, 1989). Significantly more cumulus-enclosed oocytes were fertilized and then developed to the blastocyst stage in vitro when compared with cumulus-free oocytes (Shioya et al, 1988;Mochizuki et al, 1991). A high proportion of abnormal fertilization was found when cumulus-free oocytes were used for in vitro fertilization (Vanderhyden and Armstrong, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

Zhang

Jiang

Wozniak

et al. 1995

Molecular Reproduction Devel

187

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Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15-18 hr of sperm-oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0, 7, 20, and 48 hr. The cumulus-free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, and blastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures. Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4-26% vs. 93-96%), fertilization (0-9% vs. 91-92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58-60% and 71%, respectively, vs. 91-92% for controls), cleavage development (40-47% and 53-54% vs. 74-78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co-culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

Zhang

Jiang

Wozniak

et al. 1995

Molecular Reproduction Devel

187

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“…Oocytes denuded of cumulus cells before undergoing in vitro maturation (IVM) have limited developmental capability [12], and development of bovine embryos after IVM is well correlated with the size of follicles from which the oocytes have been obtained [13] and the number of cumulus cell layers surrounding the oocytes [14]. Inclusion of extra follicle (granulosa) cells during IVM of corona-enclosed oocytes has been reported to increase embryonic development from ovine and bovine oocytes [15,16]. Oocyte viability and developmental capacity after IVM may therefore be influenced by the size and metabolic activity of the cumulus oophoruscorona cell complex, which in turn may be determined by intrafollicular levels of gonadotropins, steroids, and/or growth factors.…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of Insulin-Like Growth Factor-I and Follicle-Stimulating Hormone on Proliferation and Differentiation of Bovine Cumulus Cells and Granulosa Cells1

Armstrong

Xia

Gannes

et al. 1996

Biology of Reproduction

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Effects of gonadotropins and peptide growth factors on 3H-thymidine incorporation, as an indicator of proliferation of granulosa cells and cumulus cells from bovine ovarian follicles during in vitro culture, were determined. Incorporation of 3H-thymidine in cumulus cells during 24-h culture in protein-free medium was about 100-fold greater than in granulosa cells from small follicles. Small but significant (p < 0.05) increases in incorporation of 3H-thymidine in cumulus cells (2.5-fold), but not in granulosa cells, resulted from addition of FSH, LH, or epidermal growth factor to culture media. Much larger, highly significant (p < 0.01) increases in 3H-thymidine incorporation resulted from exposure of both cumulus cells (18-fold) and granulosa cells (6-fold) to insulin-like growth factor-I (IGF-I). Addition of IGF-I together with FSH resulted in additive increases in 3H-thymidine incorporation in granulosa cells over a wide range of each agonist. In contrast, addition of FSH markedly decreased the stimulatory effect of a maximally effective concentration of IGF-I on incorporation in cumulus cells. Addition of estradiol, either alone or with IGF-I, had no consistent effect on 3H-thymidine incorporation in either cumulus cells or granulosa cells. Differentiated functions of cumulus and granulosa cells, including cumulus expansion (an indicator of hyaluronic acid secretion) and progesterone secretion, were investigated under the same culture conditions. FSH was highly effective in stimulation of cumulus cell expansion, with maximal expansion occurring at the lowest concentration tested (0.04 micrograms/ml). LH was effective only at considerably higher concentrations consistent with degree of contamination with FSH. IGF-I was ineffective in inducing cumulus expansion, either alone or together with FSH. FSH and IGF-I by themselves each stimulated progesterone secretion by both cumulus and granulosa cells, with further additive stimulatory effects by the two agents in combination. On a per cell basis, cumulus cells produced considerably more progesterone than granulosa cells under both basal and hormone-stimulated conditions. The results suggest that IGF-I has a dual role in follicle regulation--that it acts additively with FSH as an autocrine regulator of granulosa cell growth and, in the absence of FSH, as a paracrine regulator of cumulus cell proliferation in small antral follicles. The effect of FSH, by inducing cumulus expansion, may terminate further cumulus cell proliferation before ovulation.

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“…1991). It has been shown that cumulus‐denuded oocytes can complete meiotic maturation in several species (Binor and Wolf 1979; Magnusson 1980; Yamauchi and Nagai 1999), but significantly more cumulus‐enclosed oocytes were fertilized with spermatozoa and developed to the blastocyst stage in vitro when compared with cumulus‐denuded oocytes (Staigmiller and Moor 1984; Vanderhyden and Armstrong 1989; Mochizuki et al. 1991; Yamauchi and Nagai 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the cumulus cells stabilize the disruption of cortical granules (CG), and their removal at various times during maturation results in a premature migration and partial exocytosis of CG (Galeati et al 1991). It has been shown that cumulusdenuded oocytes can complete meiotic maturation in several species (Binor and Wolf 1979;Magnusson 1980;Yamauchi and Nagai 1999), but significantly more cumulus-enclosed oocytes were fertilized with spermatozoa and developed to the blastocyst stage in vitro when compared with cumulus-denuded oocytes (Staigmiller and Moor 1984;Vanderhyden and Armstrong 1989;Mochizuki et al 1991;Yamauchi and Nagai 1999). A high proportion of abnormal fertilization was found when cumulus-denuded oocytes were used for IVF (Vanderhyden and Armstrong 1989).…”

Section: Introductionmentioning

confidence: 99%

Effect of the Removal of Cumulus Cells on the Nuclear Maturation, Fertilization and Development of Porcine Oocytes

Wongsrikeao

Kaneshige

Ooki

et al. 2005

Reprod Domestic Animals

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The present study was conducted to investigate the effects of attachment of cumulus cells to porcine oocytes during the process of maturation and fertilization on the nuclear maturation, fertilization and subsequent development after in vitro fertilization (IVF). In the first experiment, the cumulus cells were removed from cumulus-oocyte complexes (COCs) at 0, 24 and 42 h after the onset of maturation culture and were then cultured until reaching 42 h of cultivation. In the second experiment, COCs were denuded as described in the first experiment, then fertilized and cultured for 7 days. As a control, cumulus cells were allowed to maintain attachment to the oocytes until the end of IVF. The proportion of oocytes reaching metaphase II significantly increased with the delay in the removal treatment of cumulus cells. The proportion of normal fertilization gradually increased with delay in the removal treatment of cumulus cells from COCs until the end of IVF. However, no significant difference in the proportion of normal fertilization was found between the 42-h and control groups. The removal treatment of cumulus cells in the 0- and 24-h group significantly (p < 0.05) decreased the proportion of cleaved embryos when compared with the control, and none of them developed to the blastocyst stage. The proportion of development to the blastocyst stage was significantly higher (p < 0.05) in the control group than in the 42-h group (18.1% vs 12.4%; p < 0.05). The present study indicates that the attachment of cumulus cells to the oocyte during maturation and fertilization is important to support oocyte nuclear maturation, fertilization and subsequent embryo development. Particularly, the attachment of cumulus cells to the oocyte during IVF promotes embryonic development.

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Effect of the number of granulosa cells added to culture medium for in vitro maturation, fertilization and development of bovine oocytes

Cited by 43 publications

References 29 publications

Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

Differential Effects of Insulin-Like Growth Factor-I and Follicle-Stimulating Hormone on Proliferation and Differentiation of Bovine Cumulus Cells and Granulosa Cells1

Effect of the Removal of Cumulus Cells on the Nuclear Maturation, Fertilization and Development of Porcine Oocytes

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