“…The sequence in this region can form a stem-loop next to the 3' end of the primer sequence and the deletion is assumed to be an artefact of reverse transcription. The corresponding R N A and aa sequences fi'om each of the five cucumovirus strains for which complete sequences of RNAs 1-3 have been reported (Rizzo & Palukaitis, 1988Owen et al, 1990;Rezaian et al, 1984Rezaian et al, , 1985Davies & Symons, 1988;Nitta et al, 1988a;Kataoka et al, 1990a, b;O'Reilly et al, 1991 ;Bernal et al, 1991 ;Moriones et al, 1991 b;Karasawa et al, 1991Karasawa et al, , 1992 were aligned globally with those of CMV-Ix using the Clustal algorithm (Higgins & Sharp, 1989) Moriones et al, 1991b;Karasawa et al, 1991Karasawa et al, , 1992. The sequence of the untranslated RNA at the 5' end of CMV-Ix RNAs is very similar in sequence and structure to the analogous regions of the subgroup 1 CMV-Fny and CMV-Y.…”