Nucleotide sequence of tomato aspermy virus RNA 2

Abstract

RNA 2 of the V strain of tomato aspermy virus (TAV) consists of 3074 nucleotides and contains one open reading frame of 2487 nucleotides. Thus, it resembles RNA 2 of cucumber mosaic virus (CMV) strains Q and Fny (62 % identical to both), brome mosaic virus (42 % identical) and cowpea chlorotic mottle virus (40% identical). In comparisons between amino acid sequences, three different regions of similarity could be distinguished. These were the central part (amino acids 224 to 757 for V-TAV), which was most similar among the four viruses, and the N and C ends; sequences conserved among RNA polymerase species were found in the C half of the central part. Hydrophobicity patterns, and distributions of acidic and basic amino acids in the proteins encoded by V-TAV RNA 2, Q-CMV RNA 2 and Fny-CMV RNA 2 were very similar except at the extreme ends of the molecules. Structures that have been reported to act as regulatory signals for minus-and plus-strand synthesis were found in the 5' and 3' non-coding regions of the RNA.

The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs

“…The sequence in this region can form a stem-loop next to the 3' end of the primer sequence and the deletion is assumed to be an artefact of reverse transcription. The corresponding R N A and aa sequences fi'om each of the five cucumovirus strains for which complete sequences of RNAs 1-3 have been reported (Rizzo & Palukaitis, 1988Owen et al, 1990;Rezaian et al, 1984Rezaian et al, , 1985Davies & Symons, 1988;Nitta et al, 1988a;Kataoka et al, 1990a, b;O'Reilly et al, 1991 ;Bernal et al, 1991 ;Moriones et al, 1991 b;Karasawa et al, 1991Karasawa et al, , 1992 were aligned globally with those of CMV-Ix using the Clustal algorithm (Higgins & Sharp, 1989) Moriones et al, 1991b;Karasawa et al, 1991Karasawa et al, , 1992. The sequence of the untranslated RNA at the 5' end of CMV-Ix RNAs is very similar in sequence and structure to the analogous regions of the subgroup 1 CMV-Fny and CMV-Y.…”

Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA3.

“…Partial sequences of some TAV strains are also available (Moriones et al, 1991 ;O'Reilly et al, 1991). Only the complete sequence of the Japanese strain of PSV (Karasawa et al, 1991(Karasawa et al, , 1992 has been published.…”

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

“…Other cucumovirus (1-TAV, LS-CMV, Fny-CMV), stocks in our laboratory, have been described (Moriones et al, 1991 ;Owen & Palukaitis, 1988).…”