Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12

Deeley, M C; Yanofsky, Charles

doi:10.1128/jb.147.3.787-796.1981

Cited by 156 publications

(63 citation statements)

References 32 publications

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“…Preliminary work has shown that in EC0883 Tn1732 is inserted in the t n a A gene at approximately 84 min on the E. coli map (results not shown). This gene encodes the tryptophanase enzyme and is the basis of the indole test for E. colz (Deeley and Yanofsky 1981). This region of the chromosome encodes no functions known to be involved in potassium transport.…”

Section: Survival Of Antiblotlc-resistant Mutantsmentioning

confidence: 99%

The survival of bacteria exposed to desiccation on surfaces associated with farm buildings

Bale

Bennett

Beringer

et al. 1993

Journal of Applied Bacteriology

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The survival of 11 species of Gram-negative and Gram-positive bacteria was examined on different surfaces exposed to desiccation. There were large variations between species; Pseudomonas spp. and Rhizobium leguminosarum biovars survived for less than 2 d, whilst Enterococcus spp. survived for more than 11 weeks. The type of surface on to which the bacteria were deposited affected survival, but with different effects between species. In addition the survival of spontaneous nalidixic acid-resistant (Nal-r) mutants of a natural Escherichia coli isolate were compared. Overall the differences were slight, but of seven resistant mutants, five survived better than the parent whilst one survived less well. Nine transposon insertion derivatives of one of the Nal-r mutants (ECO80) which survived better than the parent were compared; all survived similarly to the parent except ECO883 which survived less well. The growth characteristics of ECO883 and ECO80 were compared; at high osmotic pressures (> 0.4 mol 1-1 NaCl) ECO883 grew more slowly and showed a longer lag time than the parent. Of the osmoregulatory functions studied, ECO883 appeared to be altered with respect to K+ transport or accumulation, although the transposon insertion had occurred in a gene distant from known K+ transport genes.

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Section: Survival Of Antiblotlc-resistant Mutantsmentioning

confidence: 99%

The survival of bacteria exposed to desiccation on surfaces associated with farm buildings

Bale

Bennett

Beringer

et al. 1993

Journal of Applied Bacteriology

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“…The amino acid sequence deduced from the tnaA gene in E. tarda was 89% identical to that deduced from tnaA in E. coli (471 amino acids). As seen in the tna operon of E. coli (Deeley and Yanofsky, 1981), the tnaA gene was also cotranscribed with tnaB in E. tarda, but the upstream regions of tnaA do not contain tanL or tnaC. The finding suggests that tna operon regulation in E. tarda is different from that in E. coli.…”

Section: Discussionmentioning

confidence: 87%

“…Subsequently, the tnaA gene was cloned into pUCm-T and sequenced. The deduced amino acid sequences of the tnaA gene in LTB-4 was identical to that in E. tarda EIB202 (Wang et al, 2009) and 89% identical to that in E. coli K-12 (Deeley and Yanofsky, 1981).…”

Section: Cloning Of Tnaa Genementioning

confidence: 79%

Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence

Yin

Yang

et al. 2011

Environ Microbiol Rep

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A fish pathogen Edwardsiella tarda LTB-4 produced various indole alkaloids, including indole, 2-(1H-indol-3-yl)ethanol, 4-di(1H-indol-3-yl)methylphenol, tri(1H-indol-3-yl)methane and 2-[2,2-bis(1H-indol-3-yl)]ethylphenylamine. Indole was the most abundant among these indole alkaloids. E. tarda LTB-4 produced indoles during its whole growth phase and maintained a high level (around 35.5 µM) during the stationary phase. The relevant tryptophanase (TnaA) gene tnaA was cloned from LTB-4 and conditionally expressed in Escherichia coli; the recombinant TnaA catalysed L-tryptophan to indole. A tnaA in-frame deletion mutant ΔtnaA was constructed through double cross-over allelic exchange by means of the suicide vector pRE118; deletion of tnaA caused some phenotypic changes including decreased swarming and twitching motility, lipopolysaccharide production and multiple antibiotic resistances. Also, subtherapeutic doses of chloromycetin, carbenicillin and tetracyline could cause the decrease of bacterial growth, but greatly induce the production of indole by E. tarda. Most importantly, attenuated virulence of the ΔtnaA mutant to zebra fish by increasing the LD50 for about 55-fold indicated that TnaA involved in the virulence of E. tarda.

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“…All of these mutations occur in a small region of tnaC previously associated with the constitutive expression of the entire operon (Konan & Yanofsky, 2000;Gong & Yanofsky, 2003;Trabuco et al, 2010). The operon consists of the three genes tnaC, which encodes a cis-regulatory leader peptide (Gish & Yanofsky, 1995), tnaA which encodes the enzyme tryptophanase (Deeley & Yanofsky, 1981), and tnaB, which encodes a tryptophan permease (Edwards & Yudkin, 1982). Tryptophanase catalyses a variety of reactions that involve tryptophan and indole, which has not only metabolic but also signalling functions (Hu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

The role of recombination in evolutionary adaptation of Escherichia coli to a novel nutrient

Chu

Sprouffske

Wagner

2017

J of Evolutionary Biology

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The benefits and detriments of recombination for adaptive evolution have been studied both theoretically and experimentally, with conflicting predictions and observations. Most pertinent experiments examine recombination's effects in an unchanging environment and do not study its genomewide effects. Here, we evolved six replicate populations of either highly recombining R or lowly recombining R E. coli strains in a changing environment, by introducing the novel nutrients L-arabinose or indole into the environment. The experiment's ancestral strains are not viable on these nutrients, but 130 generations of adaptive evolution were sufficient to render them viable. Recombination conferred a more pronounced advantage to populations adapting to indole. To study the genomic changes associated with this advantage, we sequenced the genomes of 384 clones isolated from selected replicates at the end of the experiment. These genomes harbour complex changes that range from point mutations to large-scale DNA amplifications. Among several candidate adaptive mutations, those in the tryptophanase regulator tnaC stand out, because the tna operon in which it resides has a known role in indole metabolism. One of the highly recombining populations also shows a significant excess of large-scale segmental DNA amplifications that include the tna operon. This lineage also shows a unique and potentially adaptive combination of point mutations and DNA amplifications that may have originated independently from one another, to be joined later by recombination. Our data illustrate that the advantages of recombination for adaptive evolution strongly depend on the environment and that they can be associated with complex genomic changes.

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Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12

Cited by 156 publications

References 32 publications

The survival of bacteria exposed to desiccation on surfaces associated with farm buildings

The survival of bacteria exposed to desiccation on surfaces associated with farm buildings

Mutation of tryptophanase gene tnaA in Edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence

The role of recombination in evolutionary adaptation of Escherichia coli to a novel nutrient

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