Nucleotide sequence of the rplJL operon and the deduced primary structure of the encoded L10 and L7/L12 proteins of Salmonella typhimurium compared to that of Escherichia coli

Zhyvoloup, A. N.; Woodmaska, M. I.; Kroupskaya, I. V.; Paton, E. B.

doi:10.1093/nar/18.15.4620

Cited by 10 publications

(2 citation statements)

References 3 publications

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“…1D). We investigated more precisely the relationships between P2A1 and L7/L12 proteins from different sources: chloroplasts from Arabidopsis thaliana (ear cress) (28), Spinacia oleracea (spinach) (29), Nicotiana tabacum (common tobacco) and Nicotiana sylvestris (wood tobacco) (30,31), and prokaryotes (Bacillus stearothermophilus and Bacillus subtilis (32), Escherichia coli (33), Salmonella typhimurium (34), and Synechocystis sp. (35)).…”

Section: Resultsmentioning

confidence: 99%

A Delayed-early Response Nuclear Gene Encoding MRPL12, the Mitochondrial Homologue to the Bacterial Translational Regulator L7/L12 Protein

Marty

Fort

1996

Journal of Biological Chemistry

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We have characterized a new delayed-early response mRNA encoding a 21-kDa product (MRPL12) that accumulates during the G1 phase of growth-stimulated cells. MRPL12 is the mammalian homologue to chloroplastic and bacterial L12 ribosomal proteins. Immunofluorescence microscopy and cell fractionation indicate a predominant mitochondrial localization in various mammalian cell lines. The NH2-terminal 49 amino acids are necessary and sufficient to target the protein within the mitochondria and are probably cleaved off during import. MRPL12 proteins associated in vitro and cofractionate with ribosomal structures, as is the case for prokaryotic L12 proteins. Expression of a dominant inhibitory truncated protein leads to a severe reduction in cell growth by inhibiting mitochondrial ATP production. MRPL12 is the first mammalian mitochondrial ribosomal protein to be characterized.

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Section: Resultsmentioning

confidence: 99%

A Delayed-early Response Nuclear Gene Encoding MRPL12, the Mitochondrial Homologue to the Bacterial Translational Regulator L7/L12 Protein

Marty

Fort

1996

Journal of Biological Chemistry

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“…The conservation of the sequences encoding the protein L7/L12 was also seen when comparing the B. melitensis sequence to that of other organisms. DNA sequence homologies varied between 62 and 65%, whereas amino acid similarities varied from 70 to 77% in Gram-negative eubacteria such as Escherichia coli [11], Salmonella typhimurium [14], Thermotoga maritima [15], and Gram-positive eubacteria such as Mycobacterium boris [16]. As much as 62.9% similarity was found between the L7/L12 amino acid sequences of B. melitensis and of Euglena gracilis chloroplast [17].…”

Section: Resultsmentioning

confidence: 99%

Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein

Bachrach

1994

FEMS Microbiology Letters

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DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.

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The genome of the non-cultured, bacterial-like organism associated with citrus greening disease contains thenusG-rplKAJL-rpoBC gene cluster and the gene for a bacteriophage type DNA polymerase

Villechanoux

Garnier

Laigret

et al. 1993

Current Microbiology

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We have recently cloned three DNA fragments (In-2.6, In-1.0, and In-0.6) of the non-cultured, bacterial-like organism (BLO) associated with citrus greening disease. Nucleotide sequence determination has shown that fragment In-2.6 is part of the rplKAJL-rpoBC gene cluster, a well-known operon in eubacteria. The DNA fragment upstream of and partially overlapping with In-2.6 could be isolated and was shown to be the nusG gene. In Escherichia coli, nusG is also immediately upstream of rplKAJL-rpoBC. Fragment In-1.0 carries the gene for a bacteriophage type DNA polymerase. Fragment In-0.6 could not be identified. When In-2.6 was used, at high stringency, as a probe to detect greening BLO strains in infected plants, hybridization was obtained with all Asian strains tested, but not with the African strain examined. At lower stringencies, In-2.6 was able to detect also the African strain. The implications of these results in the taxonomical position of the greening BLO are discussed.

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Nucleotide sequence of the rplJL operon and the deduced primary structure of the encoded L10 and L7/L12 proteins of Salmonella typhimurium compared to that of Escherichia coli

Cited by 10 publications

References 3 publications

A Delayed-early Response Nuclear Gene Encoding MRPL12, the Mitochondrial Homologue to the Bacterial Translational Regulator L7/L12 Protein

A Delayed-early Response Nuclear Gene Encoding MRPL12, the Mitochondrial Homologue to the Bacterial Translational Regulator L7/L12 Protein

Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein

The genome of the non-cultured, bacterial-like organism associated with citrus greening disease contains thenusG-rplKAJL-rpoBC gene cluster and the gene for a bacteriophage type DNA polymerase

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