Nucleotide sequence of the
            <i>nifH</i>
            gene coding for nitrogen reductase in the acetic acid bacterium
            <i>Acetobacter diazotrophicus</i>

Franke,; Fegan,; Hayward,; Sly,

doi:10.1046/j.1472-765x.1998.00258.x

Cited by 13 publications

(7 citation statements)

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“…Fifthly, according to Dutta & Gachhui (2007), the major difference between the type strain of Gluconacetobacter kombuchae and related gluconacetobacters was its ability to fix nitrogen. These authors confirmed the presence of the nifH gene in Gluconacetobacter kombuchae by PCR with the degenerate primers 19F and 407R (Franke et al, 1998) and sequencing of the amplified PCR product (Dutta & Gachhui, 2007). In this study, the four strains all generated a PCR product of the same size (~600 bp) using the degenerate primers used by Dutta & Gachhui (2007) (data not shown), indicating the presence of the nifH gene in all of these strains.…”

Section: Strain Numberssupporting

confidence: 66%

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Cleenwerck

Wachter

González

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Amplified fragment length polymorphism (AFLP) DNA fingerprinting was investigated as a tool for fast and accurate identification of acetic acid bacteria (AAB) to the species level. One hundred and thirty five reference strains and 15 additional strains, representing 50 recognized species of the family Acetobacteraceae, were subjected to AFLP analysis using the restriction enzyme combination ApaI/ TaqI and the primer combination A03/T03. The reference strains had been previously subjected to either DNA-DNA hybridization or 16S-23S rRNA spacer region gene sequence analysis and were regarded as being accurately classified at the species level. The present study revealed that six of these strains should be reclassified, namely Gluconacetobacter europaeus LMG 1518 and Gluconacetobacter xylinus LMG 1510 as Gluconacetobacter xylinus and Gluconacetobacter europaeus, respectively; Gluconacetobacter kombuchae LMG 23726 T as Gluconacetobacter hansenii; and Acetobacter orleanensis strains LMG 1545, LMG 1592 and LMG 1608 as Acetobacter cerevisiae. Cluster analysis of the AFLP DNA fingerprints of the reference strains revealed one cluster for each species, showing a linkage level below 50 % with other clusters, except for Acetobacter pasteurianus, Acetobacter indonesiensis and Acetobacter cerevisiae. These three species were separated into two, two, and three clusters, respectively. At present, confusion exists regarding the taxonomic status of Gluconacetobacter oboediens and Gluconacetobacter intermedius; the AFLP data from this study supported their classification as separate taxa. The 15 additional strains could all be identified at the species level. AFLP analysis further revealed that some species harboured genetically diverse strains, whereas other species consisted of strains showing similar banding patterns, indicating a more limited genetic diversity. It can be concluded that AFLP DNA fingerprinting is suitable for accurate identification and classification of a broad range of AAB, as well as for the determination of intraspecific genetic diversity.Acetic acid bacteria (AAB) are Gram-negative, coccoid or rod-shaped, obligate aerobic bacteria that have the ability to incompletely oxidize a wide range of carbohydrates, alcohols and sugar alcohols. They are involved in the production of several fermented foods and beverages, either in a beneficial (vinegars, cocoa-based products, Kombucha and nata de coco) or detrimental (spoilage of beer, wine and cider) manner and are also used in the production of commercially important fine chemicals and bacterial cellulose. Recently, some AAB have beenAbbreviations: AAB, acetic acid bacteria; AFLP, amplified fragment length polymorphism; ERIC enterobacterial repetitive intergenic consensus sequences; FAFLP, fluorescent amplified fragment length polymorphism; RAPD, randomly amplified polymorphic DNA; REP, Repetitive extragenic palindromic sequences.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of LMG 23726 T is AM999342.A supplementary figure showing the (GTG...

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Section: Strain Numberssupporting

confidence: 66%

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Cleenwerck

Wachter

González

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…1 C and D and SI Appendix, Figs. S1 and S2) and its genome contains a set of genes associated with nitrogen fixation (SI Appendix, Table S1), we were unable to find genes homologous to G. diazotrophicus nifHDK, which form the structural subunits of the nitrogenase complex (47). This either suggests that despite careful handling, very low level nitrogen contamination may have been present, allowing K. rhaeticus to produce cellulose in LGI medium or, alternatively, that K. rhaeticus uses a different set of nitrogenase genes for nitrogen fixation, as alternative nitrogenases have been isolated in other species (48).…”

Section: Discussionmentioning

confidence: 99%

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Florea

Hagemann

Santosa

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

189

216

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Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae. Its high strength, purity, and biocompatibility make it of great interest to materials science; however, precise control of its biosynthesis has remained a challenge for biotechnology. Here we isolate a strain of Komagataeibacter rhaeticus (K. rhaeticus iGEM) that can produce cellulose at high yields, grow in low-nitrogen conditions, and is highly resistant to toxic chemicals. We achieved external control over its bacterial cellulose production through development of a modular genetic toolkit that enables rational reprogramming of the cell. To further its use as an organism for biotechnology, we sequenced its genome and demonstrate genetic circuits that enable functionalization and patterning of heterologous gene expression within the cellulose matrix. This work lays the foundations for using genetic engineering to produce cellulose-based materials, with numerous applications in basic science, materials engineering, and biotechnology.

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“…Since the PCR primers were designed from the conserved regions of nifD genes, there was no possibility of amplifying other nif genes (Ueda et al 1995). The nif genes only occur in nitrogen-fixing microorganisms and have been used to monitor the presence of these diazotrophs in pure cultures (Franke et al 1998), as well as in plants (Lovell et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp. GR9

Govindarajan

Kwon

Weon

2006

World J Microbiol Biotechnol

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A group of endophytic diazotrophs were isolated from surface-sterilized roots and stems of different sugarcane varieties in the Tamilnadu region of India. From these, four isolates were selected, based on the highest acetylene reduction activity. Gene-specific PCR amplification confirmed the presence of nif-D genes in those isolates. The 16S rRNA sequence of isolates GR4 and GR7 had a 99.5% sequence similarity to the Pseudomonas sp. pDL01 (AF125317) and 16S rDNA sequence of isolate GR3 had a 100% similarity to that of Burkholderia vietnamiensis (AY973820). The 16S rDNA sequence of isolate GR9 was 99.79% similar to that of the Klebsiella pneumoniae type strain (KPY17657). Colonization by the isolates was confirmed using micropropagated sugarcane and sterile rice seedlings. Isolate GR9, identified as Klebsiella pneumoniae, was consistently more active in reducing acetylene as compared with the other isolates. The effects of GR9 and the sugarcane diazotroph Gluconacetobacter diazotrophicus were compared in inoculated micropropagated sugarcane plantlets. The effects of K. pneumoniae GR9, and four other diazotrophs, G. diazotrophicus, Herbaspirillum seropedicae, Azospirillum lipoferum 4B, and Burkholderia vietnamiensis in inoculated rice seedlings were compared. GR9 alone or in combination with the other diazotrophs performed best under pot conditions. The combined effects of nitrogen fixation and endophytic colonization of this diazotroph may be useful for the development of bio-inoculants.

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Nucleotide sequence of the nifH gene coding for nitrogen reductase in the acetic acid bacterium Acetobacter diazotrophicus

Cited by 13 publications

References 1 publication

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Isolation, molecular characterization and growth-promoting activities of endophytic sugarcane diazotroph Klebsiella sp. GR9

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