Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Florea, Michael; Hagemann, Henrik; Santosa, Gabriella; Abbott, James; Micklem, Chris N.; Spencer-Milnes, Xenia; Garcia, Laura de Arroyo; Paschou, Despoina; Lazenbatt, Christopher; Kong, Deze; Chughtai, Haroon; Jensen, Kirsten; Freemont, Paul S.; Kitney, R.I.; Reeve, Benjamin; Ellis, Tom

doi:10.1073/pnas.1522985113

Cited by 189 publications

(230 citation statements)

References 58 publications

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“…Synthetic biology research and applications to date have mostly been focused on the engineering of homogeneous or monoclonal designer cell populations to perform functions ranging from biocomputation 1 , to bioproduction of biomaterials 2 and chemicals 3 , to biosensing 4,5 among others. De novo implementation of genetic circuits of increasing complexity and size in living cells is an important challenge in synthetic biology.…”

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confidence: 99%

Tools for engineering coordinated system behaviour in synthetic microbial consortia

Kylilis

Stan

Polizzi

2017

Preprint

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Advancing synthetic biology to the multicellular level requires the development of multiple orthogonal cell-to-cell communication channels to propagate information with minimal signal interference. The development of quorum sensing devices, the cornerstone technology for building microbial communities with coordinated system behaviour, has largely focused on reducing signal leakage between systems of cognate AHL/transcription factor pairs. However, the use of noncognate signals as a design feature has received limited attention so far. Here, we demonstrate the largest library of AHL-receiver devices constructed to date with all cognate and non-cognate chemical signal interactions quantified and we develop a software tool that allows automated selection of orthogonal chemical channels. We use this approach to identify up to four orthogonal channels in silico and experimentally demonstrate the simultaneous use of three channels in coculture. The development of multiple non-interfering cell-to-cell communication channels will facilitate the design of synthetic microbial consortia for novel applications including distributed biocomputation, increased bioprocess efficiency, cell specialisation, and spatial organisation.

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mentioning

confidence: 99%

Tools for engineering coordinated system behaviour in synthetic microbial consortia

Kylilis

Stan

Polizzi

2017

Preprint

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“…[121] Moreover, combinatorial approaches that are based on pathway shuffling are pursued to further explore the chemical diversity of natural products. [123] [123] …”

Section: Angewandte Chemiementioning

confidence: 99%

Synthetic Biology—The Synthesis of Biology

2017

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Synthetic biology concerns the engineering of man-made living biomachines from standardized components that can perform predefined functions in a (self-)controlled manner. Different research strategies and interdisciplinary efforts are pursued to implement engineering principles to biology. The "top-down" strategy exploits nature's incredible diversity of existing, natural parts to construct synthetic compositions of genetic, metabolic, or signaling networks with predictable and controllable properties. This mainly application-driven approach results in living factories that produce drugs, biofuels, biomaterials, and fine chemicals, and results in living pills that are based on engineered cells with the capacity to autonomously detect and treat disease states in vivo. In contrast, the "bottom-up" strategy seeks to be independent of existing living systems by designing biological systems from scratch and synthesizing artificial biological entities not found in nature. This more knowledge-driven approach investigates the reconstruction of minimal biological systems that are capable of performing basic biological phenomena, such as self-organization, self-replication, and self-sustainability. Moreover, the syntheses of artificial biological units, such as synthetic nucleotides or amino acids, and their implementation into polymers inside living cells currently set the boundaries between natural and artificial biological systems. In particular, the in vitro design, synthesis, and transfer of complete genomes into host cells point to the future of synthetic biology: the creation of designer cells with tailored desirable properties for biomedicine and biotechnology.

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“…The development of genetically modified strains might improve the structural characteristics of BC and its yield. Synthetic biology strategies are now being used to optimize BC-producing strains (Florea et al, 2016).…”

Section: Challenges and Outlookmentioning

confidence: 99%

Bacterial cellulose as a potential bioleather substitute for the footwear industry

García¹,

Prieto

2018

Microbial Biotechnology

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Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Cited by 189 publications

References 58 publications

Tools for engineering coordinated system behaviour in synthetic microbial consortia

Tools for engineering coordinated system behaviour in synthetic microbial consortia

Synthetic Biology—The Synthesis of Biology

Bacterial cellulose as a potential bioleather substitute for the footwear industry

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