Nucleotide Sequence of the 3'-Terminal Region of Potato Virus T RNA.

Ochi, Masanao; Kashiwazaki, Satoshi; Hiratsuka, Kazuyuki; Namba, Susumu; Tsuchizaki, Tsuneo

doi:10.3186/jjphytopath.58.416

Cited by 21 publications

(15 citation statements)

References 34 publications

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“…The 21"6 kDa and 36"5 kDa products were identified as the CP and movement protein (MP), respectively. GVB CP showed substantial sequence homology with the comparable products of apple chlorotic leaf spot (ACLSV) and potato T (PVT) viruses (German et al, 1990;Ochi et al, 1992;Sato et al, 1993), two definitive species of the genus Trichovirus .…”

Section: Introductionmentioning

confidence: 99%

The nucleotide sequence and genomic organization of grapevine virus B

Saldarelli¹,

Minafra²,

Martelli³

1996

Journal of General Virology

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Section: Introductionmentioning

confidence: 99%

The nucleotide sequence and genomic organization of grapevine virus B

Saldarelli¹,

Minafra²,

Martelli³

1996

Journal of General Virology

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“…encoding proteins of M r > 29K, 40K (putative movement protein) and 24K (coat protein) were found in the T-terminal region of PVT RNA (Ochi et al, 1992).…”

mentioning

confidence: 99%

“…Recently, the complete nucleotide sequence of the genome of ASGV and the nucleotide sequence of the 3'-terminal region of RNA of potato virus T (PVT), another member of the capillovirus group, were reported (Yoshikawa et al, 1992;Ochi et at., 1992). Surprisingly, the genome organizations of these two viruses are quite different.…”

mentioning

confidence: 99%

Striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses

Yoshikawa¹,

Imaizumi²,

Takahashi³

et al. 1993

Journal of General Virology

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“…These viruses have been distinguished from closteroviruses (Candresse, 1994;Dolja et al, 1994) by differences in particle length, biological, molecular, physiochemical and cytopathological properties . In addition to the complete nucleotide sequences of two ACLSV isolates, three T-terminal sequences are available for the definitive trichovirus potato virus T (PVT; Ochi et al, 1992) and the two tentative species grapevine viruses A and B (GVA, GVB; Minafra et al, 1994) respectively. Although capilloviruses and trichoviruses appear to be closely related, the separation into two different genera is supported by their difference in genome organization and strategy of expression .…”

Section: Introductionmentioning

confidence: 99%

Cherry virus A: cDNA cloning of dsRNA, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry

Wilhelm

1995

Journal of General Virology

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The nucleotide sequence (7383 nucleotides) of a newly identified member of the genus Capillovirus, cherry virus A (CVA), was obtained from cDNA clones. The cDNA was generated from dsRNA extracted from plant tissue infected with little cherry virus (LCV). Small amounts of LCV dsRNA served as template nucleic acid and enabled the construction of a library of which, unexpectedly, 7.5% of the recombinant plasmids were specific for CVA. The genome organization of CVA resembles that of apple stem grooving virus (ASGV), the type member of the genus Capillovirus and is composed of a 266 kDa polyprotein (ORF1), a 52 kDa ORF2 located within ORF1 and a poly(A) tail. The 266 kDa ORF1 contains all the elements of a replication-related protein and has high identity with 'Sindbis-like' viruses. The ORF encodes the coat protein (CP) in the Cterminal region. The 52 kDa ORF2 has high identities with the putative viral cell-to-cell movement proteins of capiUo-and trichoviruses. The CP was identified in immunoblot analysis and estimated to have a molecular mass of 24 kDa. Antiserum was obtained by expression of antigens as fusion proteins in Escherichia coli. There is significant sequence identity between CVA CP and the corresponding proteins of other capillo-and trichoviruses. However, no serological cross-reaction was obtained in immunoblot analysis with ASGV, apple chlorotic leafspot trichovirus (ACLSV), apple stem pitting virus (ASPV) and cherry mottle leaf virus (CMLV) antisera. Flexuous filamentous CVA virions were identified in extracts of sweet cherry by immunosorbent electron microscopy (ISEM) and decorated with the antiserum to the fusion protein. CVA was identified in three cherry sources of different disease status by ISEM, immunoblot analysis and hybridization to dsRNA. CVA is not closely related to any of the currently described diseases in cherry but it has all the properties of a capillovirus. It is suggested that CVA should be classified as a new member of the genus Capillovirus.

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Nucleotide Sequence of the 3'-Terminal Region of Potato Virus T RNA.

Abstract: The partial sequence of the capillovirus potato virus T (PVT) RNA containing the 3•OE-terminal 2392 nucleotides, excluding the poly (A) tail, was determined from cloned cDNA.

Cited by 21 publications

References 34 publications

The nucleotide sequence and genomic organization of grapevine virus B

The nucleotide sequence and genomic organization of grapevine virus B

Striking similarities between the nucleotide sequence and genome organization of citrus tatter leaf and apple stem grooving capilloviruses

Cherry virus A: cDNA cloning of dsRNA, nucleotide sequence analysis and serology reveal a new plant capillovirus in sweet cherry

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