Nucleotide sequence of bovine rotavirus genomic segment 10: an RNA encoding the viral non-structural glycoprotein

Powell, K.F.H.; Gunn, Peter; Bellamy, A R

doi:10.1093/nar/16.2.763

Cited by 15 publications

(11 citation statements)

References 4 publications

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“…to six previously reported NSP4 sequences (strains Wa, A28, UK, NCDV, YM, and SA11) and five unpublished NSP4 sequences (strains RRV, RV-5, S2, M37, and RV4), including both human and animal rotaviruses [Baybutt and McCrae, 1984;Both et al, 1983;Okada et al, 1984;Ward et al, 1985;Ballard et al, 1992;Powell et al, 1988;Lopez and Arias, 1993] (E.A. Palombo and C.D.…”

Section: Discussionmentioning

confidence: 68%

“…Palombo and C.D. Kirkwood, unpublished data, 1996; U59108), and NCDV [Powell et al, 1988] (X06806) have been previously described.…”

Section: Discussionmentioning

confidence: 99%

“…Unexpectedly, the NSP4 genes of some animal rotaviruses (UK, NCDV, SA11, YM) and a G serotype 10 HRV strain recovered from a child with severe combined immunodeficiency syndrome [Baybutt and McCrae, 1984;Both et al, 1983;Ward et al, 1985;Ballard et al, 1992;Powell et al, 1988;Lopez and Arias, 1993] could be classified in one of these NSP4 genetic groups. This observation suggests a strong evolutionary relationship between the NSP4 genes of human and animal rotaviruses, which for some strains might also be explained in terms of genogroup relationships [e.g., strain YM NSP4 gene is 88% and 95% identical at the nt and aa levels, respectively, to the Wa NSP4 gene and shows significant relatedness to Wa genogroup strains by RNA-RNA hybridization (Kojima et al, 1996)].…”

Section: Discussionmentioning

confidence: 99%

“…Among common rotavirus serotypes of infants, only the strain Wa NSP4 gene sequence has previously been reported [Okada et al, 1984]. The few other published sequences are from strains isolated from human neonates, two strains isolated from a chronically infected immunodeficient child and the animal rotaviruses UK, NCDV, YM, and SA11 [Powell et al, 1988;Kirkwood et al, 1996;Baybutt and McCrae, 1984;Ballard et al, 1992;Both et al, 1983;Lopez and Arias, 1993;Ward et al, 1985].…”

Section: Introductionmentioning

confidence: 99%

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Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

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The rotavirus nonstructural glycoprotein NSP4 may represent the first identified viral enterotoxin. We have sequenced reverse transcription-polymerase chain reaction (RT-PCR)-generated fragments of 16 NSP4 genes of human rotavirus (HRV) strains from six different countries, representing seven different G and P type combinations. Based on the amount of sequence divergence between these and 11 previously sequenced NSP4 genes of human and animal rotaviruses, three distinct genetic groups could be recognized. Most strains within a group were closely related to each other at the nucleotide (nt) and amino acid (aa) levels (usually <10% divergence) but more distantly related (maximum 30.0% nt divergence and 24.7% aa divergence) to members of the other groups. Intergroup variation occurred in two highly variable regions of NSP4 (aa 16-34 and aa 131-148). The NSP4 "toxic peptide" (aa 114-135) exhibited aa variation at its carboxy terminus both within and between genetic groups. The largest group (genetic group II) contained HRV strains of subgroup II specificity (including genotypes P[8]G1, P[8]G3, P[6]G3, and P[8]G5 and serotype P8[11]G9), and the smaller group (genetic group I) contained HRV strains of subgroup I specificity (genotype P[4]G2). The NSP4 sequence of the rhesus rotavirus vaccine strain was distinct from all other strains and formed the third group (genetic group III). The NSP4 genes of animal rotaviruses UK, NCDV, and SA11 (genetic group I) and YM (genetic group II) and two possible human-animal rotavirus reassortant strains, Brazilian P[8]G5 and Indian P[11]G9 (genetic group II), could also be classified into one of these groups, suggesting a close evolutionary relationship between human and animal NSP4 genes. These results will facilitate studies of the host immune response to NSP4, which may be relevant to future HRV vaccine design.

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Section: Discussionmentioning

confidence: 68%

“…Palombo and C.D. Kirkwood, unpublished data, 1996; U59108), and NCDV [Powell et al, 1988] (X06806) have been previously described.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

et al. 1997

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“…Rotavirus YM gene 10 is 750 nucleotides in length, that is one nucleotide shorter than the same genes of other rotavirus strains sequenced (Both et al, 1983;Baybutt & McCrae, 1984;Okada et al, 1984;Ward et aI., 1985;Powell et al, 1988;Ballard et al, 1992), with the exception of human rotavirus Wa gene 10 which is also 750 nucleotides in length, and shares with the rotavirus YM gene the absence of an A residue at position 684, in the 3' non-translated region. YM strain gene 10 has a single long open reading frame that codes for NS28, a protein of 175 amino acids.…”

mentioning

confidence: 94%

Sequence analysis of rotavirus YM VP6 and NS28 proteins

López

Arias

1993

Journal of General Virology

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Topology of the non-structural rotavirus receptor glycoprotein NS28 in the rough endoplasmic reticulum.

et al. 1989

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The rotavirus non‐structural glycoprotein (NS28), the receptor for the virus core during budding into the lumen of the rough endoplasmic reticulum (RER), is 175 amino acids long and possesses an uncleaved signal sequence and two amino‐terminal glycosylation sites. Utilizing one of three potential hydrophobic domains, the protein spans the membrane only once, with the glycosylated amino‐terminal region oriented to the luminal side of the ER and the carboxy‐terminal region to the cytoplasmic side. To localize sequences involved in translocation of NS28, we constructed a series of mutations in the coding regions for the hydrophobic domains of the protein. Mutant protein products were studied by in vitro translation and by transfection in vivo. In transfected cells, all mutant forms localize to the ER, and none are secreted. In vitro, each of the three hydrophobic domains is able to associate with microsomes. However, glycosylation and proteolysis of wild‐type and mutant forms of NS28 indicates that the wild‐type protein is anchored in the membrane only by the second hydrophobic domain, leaving approximately 131 residues exposed on the cytoplasmic side for receptor ‐ ligand interaction.

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Nucleotide sequence of bovine rotavirus genomic segment 10: an RNA encoding the viral non-structural glycoprotein

Cited by 15 publications

References 4 publications

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups

Sequence analysis of rotavirus YM VP6 and NS28 proteins

Topology of the non-structural rotavirus receptor glycoprotein NS28 in the rough endoplasmic reticulum.

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