Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product

Phylogeny of the Major Head and Tail Genes of the Wide-Ranging T4-Type Bacteriophages

“…These primers flank the sequences shown in the compilation ( see Fig. 3), and they correspond respectively to the nucleotide sequences encoding T4 gene 23 amino acid (aa) residues 95 to 103 and 368 to 375 (26). The conditions used for the amplification reaction with these primers involved 28 cycles consisting of a 30-s denaturation at 96°C, a 2-min annealing at 62°C, and a 3-min extension at 72°C.…”

The genome of the pseudo T-even bacteriophages, a diverse group that resembles T4

“…Transfer to Hybond N membrane was according to Amersham. 32 P-labeled probes were prepared using Amersham's Megaprime DNA labeling system, or using PCR and radioactive primers CAP7 (5 H -TCTGGCGCAGTAACTCAGATTGGC) and CAP8 (5-TGAAGTTACCTTCACCACGACCGG), corresponding to 253 from the starting ATG on the coding strand and 1125 from the starting ATG on the non-coding strand of T4 g23, respectively (Parker et al, 1984). Hybridization conditions were 5 Â SSPE, 5Â Denhardt's and 1% (w/v) SDS, overnight at 65 C (Sambrook et al.…”

Correlation of Topographic Surface and Volume Data from Three-Dimensional Electron Microscopy

“…Assuming a protein density of 1.3 g/cm 3 the surfaces include a mass of 263.4 kDa per unit cell. This corresponds to 90.1% of the mass calculated from the known amino acid sequence (Parker et al, 1984).…”

“…6. As the calculated mass included between the surfaces corresponds to 90% of the known amino acid mass (Parker et al, 1984), it can be concluded that the resulting position of the inner surface is not far from the true position, assuming that the outer surface is correctly scaled and aligned. Therefore, it seems to be reasonable to build a 3D model by putting the surfaces into the volume according to the correlation results and setting all density outside the surfaces to zero (Fig.…”