Nucleotide Sequence of a Portion of the Autographa californica Nuclear Polyhedrosis Virus Genome Containing the EcoRI Site-rich Region (hr5) and an Open Reading Frame just 5' of the p10 Gene

Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome

“…Furthermore, the ATAAG consensus pentamer spans the positions 53 to 57 nt downstream from the last EcoRI site. This positioning is very similar to that of hr5 in relation to the p26 gene reported by Liu et al (1986) where the p26 transcript originates 56 nt downstream and the coding sequence of p26 begins 69 nt downstream from the last EcoRI site of the hrs region. A significant difference, however, is that the p26 transcript does not initiate at an ATAAG sequence.…”

Functional Studies on the p10 Gene of Autographa californica Nuclear Polyhedrosis Virus Using a Recombinant Expressing a p10-beta-Galactosidase Fusion Gene

“…The nucleotide sequence of the pl0 gene (Kuzio et al, 1984) and its 5' flanking sequence (Liu et al, 1986) are known and the mode of transcription has been investigated (Rankin et al, j.M. VLAK AND OTHERS 1986).…”

Studies on the Control Region of the p10 Gene of the Autographa californica Nuclear Polyhedrosis Virus

“…We began with plasmid pAEp26p10CAT, which was constructed by resecting the large HindlII-BgllI fragment of HindlII Q (87-5 to 89.0 map units) with Ba131 nuclease, then removing overhangs with T4 DNA polymerase, blunt end-ligating into the SmaI site of pUC13 and inserting the CAT cartridge from plasmid pCM-4 (Pharmacia) into the adjacent BamHI site. The resulting plasmid contains the hr5 enhancer (Guarino & Summers, 1986;Liu et al, 1986), the p26 control and coding region (Liu et al, 1986), the pl0 control region, and part of the pl0 coding region. Sequencing (Sanger et al, 1977;Chen & Seeburg, 1985) showed that the CAT gene is inserted in phase just after codon 4 of the p 10 gene.…”

“…The other very late gene codes for an abundant late protein of Mr 10000 (pl0) whose function is unknown, and which is dispensable in viral replication (Vlak et al, 1988). This gene has been mapped and sequenced (Kuzio et al, 1984;L/ibbert & Doerfler, 1984), and detailed mapping of the transcripts in the region of this gene has been performed (Rankin et al, 1986;Liu et al, 1986). Possee & Howard (1987) have performed insertion and deletion mutagenesis on the polyhedrin gene 5' flanking region linked to the fl-galactosidase reporter gene in a recombinant virus.…”