Studies on the Control Region of the p10 Gene of the Autographa californica Nuclear Polyhedrosis Virus

Dissimilar expression of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus polyhedrin and p10 genes

“…Linker-scan mutation analysis of the polyhedrin promoter has failed to confirm this observation, but has identified sequences downstream of the canonical promoter element that may be crucial to initiation at the transcription start site (Ooi et al, 1989). The AcMNPV pl0 promoter consists of a 70 nucleotide 5' non-coding leader sequence (Weyer & Possee, 1988;Qin et al, 1989) and an additional 30 nucleotides upstream from the transcriptional start with an enhancement function (Weyer & Possee, 1989). The 5' leaders of both promoters are extremely AT-rich (80~).…”

Nucleotide sequence and transcript mapping of the HindIII F region of the Autographa californica nuclear polyhedrosis virus genome

“…This is probably necessitated by the fact that two different RNA polymerases are active early and late in infection; the host RNA polymerase II transcribes the early genes, whereas a virus-induced RNA polymerase transcribes the late genes (Fuchs et al, 1983;Huh & Weaver, 1990). The latter polymerase recognizes its own class of promoters (Possee & Howard, 1987;Matsuura et al, 1987;Weyer & Possee, 1988;Qin et al, 1989). Therefore, the presence of both types of promoters in certain genes ensures their expression both early and late.…”

Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors

“…However both the initiation sites for pl0 and polyhedrin transcripts have been mapped within a common core sequence, ATAAG (Kuzio et al, 1984;Ltibbert & Doerfler, 1984;Howard et al, 1986;Rankin et aL, 1988), which has been found in all the late and very late genes of AcMNPV analysed so far. Mutation of this core sequence abolished the activity of both the polyhedrin and the pl0 promoter (Possee & Howard, 1987;Rankin et al, 1988;Qin et al, 1989;Weyer & Possee, 1989) suggesting that the ATAAG motif plays an important role in the regulation of the pl0 and polyhedrin very late gene expression.…”