Nucleotide Sequence of a cDNA Encoding 1-Aminocyclopropane-1-Carboxylate Oxidase from Senescing Orchid Petals

Nadeau, Jeanette A.; O’Neill, Sharman D.

doi:10.1104/pp.108.2.833

Cited by 12 publications

(3 citation statements)

References 8 publications

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“…This increase might have been partially responsible for the increase in perianth and in column ACO activity. The expression of the DenACO gene, after pollination, was similar to that of an ACO gene in Phalaenopsis, where a rapid and large up-regulation was observed in the column of pollinated flowers (Nadeau et al, 1993) and similar to that reported for Doritaenopsis (Nadeau and O'Neill, 1995), Phalaenopsis (Bui and O'Neill, 1998), Vanda (Lokkamlue and Huehne, 2013, 2020), plum (Fernandez-Otero et al, 2006, and tomato (Llop-tous et al, 2000). The DenACO gene at 6 h in the column plus ovary of the pollinated florets with the 1-MCP treatment was highly expressed throughout the study period.…”

Section: Discussionsupporting

confidence: 79%

1-Methylcyclopropene inhibits ethylene production and post-pollination changes of Dendrobium flowers

2022

ANRES

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Importance of the work: Stimulation of ethylene production by pollination causes early flower senescence and the initiation of many post-pollination developmental processes. Objectives: To investigate the effects of 1-methylcyclopropene (1-MCP) on the ethylene production and post-pollination changes of Dendrobium cv. Sonia Bom #17 florets following pollination.Materials & Methods: Open florets were treated with 1-MCP prior to pollination and monitored for ethylene production, the 1-aminocyclopropane-1 carboxylic acid (ACC) content, activities of ACC synthase (ACS) and ACC oxidase (ACO), gene expression, epinasty of florets, yellowing of the labellum, perianth venation and water-soaking of the floret tissues after pollination. Results: Treatment of florets with 1-MCP prior to pollination delayed epinasty of florets, yellowing of the labellum, perianth venation and water-soaking of florets after pollination. In addition, the 1-MCP treatment reduced ovary growth, ethylene production, ACC content and the activities of ACS and ACO in pollinated florets. The ACC content and ACO activity increased more in the column plus ovary than in the perianth of pollinated florets while ACS activity in the column plus ovary increased less than in the perianth. The DenACO gene in the perianth of non-pollinated florets with the 1-MCP treatment was expressed more highly than that of pollinated florets with the 1-MCP treatment. The DenACO gene was expressed slightly throughout the study period in the column plus ovary of pollinated florets with the 1-MCP treatment. Main finding: 1-MCP treatment delayed perianth senescence and decreased ethylene production, ACC. ACS and ACO in pollinated florets. The column was the likely source of ethylene production, while the increased ethylene production might have been partially due to an increase in DenACO gene expression.

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Section: Discussionsupporting

confidence: 79%

1-Methylcyclopropene inhibits ethylene production and post-pollination changes of Dendrobium flowers

2022

ANRES

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“…Three ACC oxidase genes have been reported as cloned (Table 1). Two genes, OAO1 and D-ACO2, were isolated from cDNA libraries of gynoecia and the senescing petals of pollinated Doritaenopsis fl owers, respectively (Nadeau et al, 1993;Nadeau and OʼNeill, 1995). Another ACC oxidase cDNA clone was isolated from senescing petals of Phalaenopsis (Lee and Huang, 1995).…”

Section: Feature Featurementioning

confidence: 99%

Orchid Biotechnology in Production and Improvement

Mudalige¹,

Kuehnle²

2004

HortSci

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“…A eDNA clone that was functionally related to the ripening and wounding process of tomato (pTOM13) was first isolated by Holdsworth et al (2). This cDNA encodes the ACC To date, many cDNAs encoding ACC oxidases have been isolated and studied from various types of plants including fruits, such as avocado (4), apple (5,6), peach (7), and kiwi (8); senescent flowers, such as carnation (9), petunia (10), mold orchid (11,12), and geranium (13);…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of a banana gene encoding 1‐aminocyclopropane‐1‐carboxylate oxidase

Huang

et al. 1997

IUBMB Life

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A cDNA encoding the banana 1‐aminocyclopropane‐l‐carboxylate (ACC) oxidase has previously been isolated from a eDNA library that was constructed by extracting poly(A)+ RNA from peels of ripening banana. This cDNA, designated as pMAO2, has 1,199 bp and contains an open reading frame of 318 amino acids. In order to identify ripening‐related promoters of the banana ACC oxidase gene, pMAO2 was used as a probe to screen a banana genomic library constructed in the λEMBL3 vector. The banana ACC oxidase MA02 gene has four exons and three introns, with all of the boundaries between these introns and exons sharing a consensus dinucleotide sequence of GT‐AG. The expression of MA02 gene in banana begins after the onset of ripening (stage 2) and continues into later stages of the ripening process. The accumulation of MAO2 mRNA can be induced by 1 μl/l exogenous ethylene, and it reached steady state level when 100 μl/1 exogenous ethylene was present.

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Nucleotide Sequence of a cDNA Encoding 1-Aminocyclopropane-1-Carboxylate Oxidase from Senescing Orchid Petals

Cited by 12 publications

References 8 publications

1-Methylcyclopropene inhibits ethylene production and post-pollination changes of Dendrobium flowers

1-Methylcyclopropene inhibits ethylene production and post-pollination changes of Dendrobium flowers

Orchid Biotechnology in Production and Improvement

Characterization and expression analysis of a banana gene encoding 1‐aminocyclopropane‐1‐carboxylate oxidase

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