Characterization and expression analysis of a banana gene encoding 1‐aminocyclopropane‐1‐carboxylate oxidase

Huang, Pung‐Ling; Do, Yi‐Yin; Huang, Fay; Thay, Te‐Shih; Chang, T. C.

doi:10.1080/15216549700202001

Cited by 9 publications

(19 citation statements)

References 12 publications

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“…3). High message levels of the MA-ACO1 gene in ripening banana fruit have previously been demonstrated by Ló pez-Gó mez et al (1997) and Huang et al (1997). The in vivo and in vitro activities of ACC oxidase are reflected in the accumulated level of ACC oxidase mRNAs in various climacteric fruits such as apple (Dong et al, 1992), tomato (Nakatsuka et al, 1997), peach (Tonutti et al, 1997), melon (Bouquin et al, 1997, and pear (Lelièvre et al, 1997b).…”

Section: Possible Mechanism Of the Rapid Decline In Ethylene Productimentioning

confidence: 87%

“…Therefore, six or more members of ACC synthase gene family may exist in banana. MA-ACO1 showed a high sequence similarity (more than 98%) to the one registered as the ACC oxidase gene related to banana ripening (Huang et al, 1997;Ló pez-Gó mez et al, 1997).…”

Section: Isolation Of Cdnas Encoding Acc Synthase and Acc Oxidase Fromentioning

confidence: 99%

“…Expression of ACC synthase genes has been investigated in many fruits, including apple (Dong et al, 1991), tomato (Olson et al, 1991;Rottmann et al, 1991;Lincoln et al, 1993;Nakatsuka et al, 1998), melon (Yamamoto et al, 1995), pear (Lelièvre et al, 1997b), passion fruit (Mita et al, 1998), and cucumber . Expression of ACC oxidase genes has also been investigated in fruits such as tomato (Barry et al, 1996;Nakatsuka et al, 1998), apple (Ross et al, 1992), melon (Balaguè et al, 1993;Yamamoto et al, 1995;Lasserre et al, 1996), kiwi (Whittaker et al, 1997), pear (Lelièvre et al, 1997b), cucumber , passion fruit (Mita et al, 1998), and banana (Huang et al, 1997;Ló pez-Gó mez et al, 1997).…”

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confidence: 99%

“…Therefore, it is important to investigate at both the biochemical and molecular levels the possible mechanism(s) involved in the sharp peak of ethylene production at the early climacteric stage in banana fruit. An ACC oxidase gene has been cloned from banana fruit and its expression pattern during fruit ripening has been characterized (Clendennen et al, 1997;Huang et al, 1997;Ló pez-Gó mez et al, 1997). However, to our knowledge, there has been no report related to the expression of ACC synthase genes, despite the fact that six sequences for banana ACC synthase have already been registered in the database.…”

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confidence: 99%

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Characterization of Ethylene Biosynthesis Associated with Ripening in Banana Fruit

et al. 1999

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We investigated the characteristics of ethylene biosynthesis associated with ripening in banana (Musa sp. [AAA group, Cavendish subgroup] cv Grand Nain) fruit. MA-ACS1 encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase in banana fruit was the gene related to the ripening process and was inducible by exogenous ethylene. At the onset of the climacteric period in naturally ripened fruit, ethylene production increased greatly, with a sharp peak concomitant with an increase in the accumulation of MA-ACS1 mRNA, and then decreased rapidly. At the onset of ripening, the in vivo ACC oxidase activity was enhanced greatly, followed by an immediate and rapid decrease. Expression of the MA-ACO1 gene encoding banana ACC oxidase was detectable at the preclimacteric stage, increased when ripening commenced, and then remained high throughout the later ripening stage despite of a rapid reduction in the ACC oxidase activity. This discrepancy between enzyme activity and gene expression of ACC oxidase could be, at least in part, due to reduced contents of ascorbate and iron, cofactors for the enzyme, during ripening. Addition of these cofactors to the incubation medium greatly stimulated the in vivo ACC oxidase activity during late ripening stages. The results suggest that ethylene production in banana fruit is regulated by transcription of MA-ACS1 until climacteric rise and by reduction of ACC oxidase activity possibly through limited in situ availability of its cofactors once ripening has commenced, which in turn characterizes the sharp peak of ethylene production.

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Section: Possible Mechanism Of the Rapid Decline In Ethylene Productimentioning

confidence: 87%

Section: Isolation Of Cdnas Encoding Acc Synthase and Acc Oxidase Fromentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization of Ethylene Biosynthesis Associated with Ripening in Banana Fruit

et al. 1999

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“…The first ACC synthase, cDNA, was isolated from zucchini using an experimental approach [10]. Since the initial reports, ACC synthase, or genomic sequences, were isolated from numerous climacteric fruits such as apple [11], papaya [12], kiwifruit [13], sugarcane [14], peach [15], banana [16], tomato [17], and pear [18]. …”

Section: Introductionmentioning

confidence: 99%

Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana

Xia

Kuan

Chiu

et al. 2016

IJMS

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Among 18 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase homologous genes existing in the banana genome there are two genes, Mh-ACO1 and Mh-ACO2, that participate in banana fruit ripening. To better understand the physiological functions of Mh-ACO1 and Mh-ACO2, two hairpin-type siRNA expression vectors targeting both the Mh-ACO1 and Mh-ACO2 were constructed and incorporated into the banana genome by Agrobacterium-mediated transformation. The generation of Mh-ACO1 and Mh-ACO2 RNAi transgenic banana plants was confirmed by Southern blot analysis. To gain insights into the functional diversity and complexity between Mh-ACO1 and Mh-ACO2, transcriptome sequencing of banana fruits using the Illumina next-generation sequencer was performed. A total of 32,093,976 reads, assembled into 88,031 unigenes for 123,617 transcripts were obtained. Significantly enriched Gene Oncology (GO) terms and the number of differentially expressed genes (DEGs) with GO annotation were ‘catalytic activity’ (1327, 56.4%), ‘heme binding’ (65, 2.76%), ‘tetrapyrrole binding’ (66, 2.81%), and ‘oxidoreductase activity’ (287, 12.21%). Real-time RT-PCR was further performed with mRNAs from both peel and pulp of banana fruits in Mh-ACO1 and Mh-ACO2 RNAi transgenic plants. The results showed that expression levels of genes related to ethylene signaling in ripening banana fruits were strongly influenced by the expression of genes associated with ethylene biosynthesis.

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Molecular Cloning and Characterization of A Novel 1-Aminocyclopropane-1-carboxylate Oxidase Gene Involved in Ripening of Banana Fruits

Thay²,

Chang³

et al. 2005

J. Agric. Food Chem.

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One novel banana fruit ripening related 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene quite different from ACC oxidase genes from other species was cloned. In contrast to other studies, the polypeptide encoded by this gene, named Mh-ACO1, lacks the putative leucine zipper motif which is conserved in all known ACC oxidases including the other previously reported banana ACC oxidase, Mh-ACO2. The locus consists of two nearly identical paralogous ACC oxidase genes arranged in opposite orientation and separated by a 3.1-kb intergenic region. The has only two introns, at positions identical to , which comprises a coding region interrupted by three introns. The predicted amino acid sequence of Mh-ACO1 shares less than 50% identity to those of ACC oxidase from other climacteric fruits, while that of Mh-ACO2 shows more than 65% homology. When expressed in Saccharomyces cerevisiae -encoded protein possessed the enzyme activity for ethylene conversion. The levels of mRNA corresponding to both and increased during fruit ripening and were induced by exogenous ethylene. We conclude that both and contribute to increased ethylene production in fruits and these two genes are differentially expressed in fruits and other organs in banana.

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Characterization and expression analysis of a banana gene encoding 1‐aminocyclopropane‐1‐carboxylate oxidase

Cited by 9 publications

References 12 publications

Characterization of Ethylene Biosynthesis Associated with Ripening in Banana Fruit

Characterization of Ethylene Biosynthesis Associated with Ripening in Banana Fruit

Global Transcriptomic Analysis of Targeted Silencing of Two Paralogous ACC Oxidase Genes in Banana

Molecular Cloning and Characterization of A Novel 1-Aminocyclopropane-1-carboxylate Oxidase Gene Involved in Ripening of Banana Fruits

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