Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene variable (V4) region among and within geographic isolates of Theileria from cattle, elk and white-tailed deer

Chae, Joon‐Seok; Lee, Joo-mook; Kwon, Oh-Deog; Holman, Patricia J.; Waghela, Suryakant D.; Wagner, G. G.

doi:10.1016/s0304-4017(97)00183-0

Cited by 60 publications

(57 citation statements)

References 27 publications

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“…SSU rRNA data indicate that both white-tailed deer and elk harbor T. cervi, as supported by this study and previous work (Chae et al, 1998). Complete nucleotide sequences from SSU rRNA genes amplified from cervine blood infected with Theileria spp.…”

Section: Discussionsupporting

confidence: 65%

“…DNA extraction and SSU rRNA gene amplification procedures for the USWTD1, USWTD2 and CNELK isolates have been previously reported (Allsopp et al, 1989;Chae et al, 1998). DNA purification and SSU rRNA gene amplification from the USWTD3 and the OKELK1 Theileria isolates were similarly executed.…”

Section: Methodsmentioning

confidence: 99%

“…Two additional T. cervi isolates (USWTD1 and USWTD2) from Texas white-tailed deer and a Canadian elk Theileria sp. isolate (CNELK) used in this study have been previously described (Chae et al, 1998). A summary of the Theileria spp.…”

Section: Methodsmentioning

confidence: 99%

“…The plasmid DNA samples were then used to identify both Type F and Type G clones for further study. Sequencing reactions for each plasmid DNA sample (Dye Teminator Cycle Sequencing Ready Reaction; PE Applied Biosystem, Norwalk, Connecticut, USA) used primer 528F to determine the SSU rRNA gene V4 variable region nucleotide sequence as previously described (Chae et al, 1998 Plasmid clones containing the entire SSU rRNA genes from USWTD1, USWTD2, and CNELK from previous work (Chae et al, 1998) were used for complete gene sequencing for these isolates. Since previously only one plasmid clone containing the WTD2 SSU rRNA gene insert was obtained (Chae et al, 1998), transformation of the original ligated plasmid into INV␣FЈ One Shot E. coli was repeated.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, SSU rRNA gene V4 variable region sequence analyses distinguished genes amplified from white-tailed deer and elk T. cervi isolates (sequence Types F and G) from genes of bovine Theileria spp. isolates (Chae et al, 1998). DNA probes to identify Theileria spp.…”

Section: Introductionmentioning

confidence: 99%

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Two Theileria Cervi Ssu Rrna Gene Sequence Types Found in Isolates From White-Tailed Deer and Elk in North America

Chae

Waghela²,

Craig³

et al. 1999

Journal of Wildlife Diseases

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Two Theileria cervi SSU rRNA gene sequence Types, F and G, from white-tailed deer (Odocoileus virginianus) and elk (Cervus elaphus canadensis) isolates in North America were confirmed. Previously, nucleotide sequencing through a single variable (V4) region showed the presence of SSU rRNA gene Types F and G in T. cervi isolates from white-tailed deer and an elk. In this study, both sequence types were found in four T. cervi isolates (two from deer and two from elk). Microheterogeneity only appeared in the Type G gene, resulting in Subtypes G1, G2 and G3. Subtype G1 was found in two elk and one white-tailed deer T. cervi isolate; Subtypes G2 and G3 were found in a white-tailed deer T. cervi isolate. The Type F SSU rRNA genes were identical in nucleotide sequence in both elk and white-tailed deer T. cervi isolates. The high degree of conservation in the Type F variable regions may be exploited to design specific oligonucleotide primers for parasite detection by the polymerase chain reaction in cervine or tick hosts.

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Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Two Theileria Cervi Ssu Rrna Gene Sequence Types Found in Isolates From White-Tailed Deer and Elk in North America

Chae

Waghela²,

Craig³

et al. 1999

Journal of Wildlife Diseases

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Non-Transforming Theileria Parasites of Ruminants

Sugimoto

Fujisaki

2002

World Class Parasites

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Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene

Chansiri

Sarataphan

2002

Parasitol Res

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Theileria type Thung Song is an indigenous hemoparasite of dairy cattle from the south of Thailand. It has previously been classified using the analysis of a comparative set of small subunit ribosomal RNA nucleotide sequences. However, the classification of this parasite is still questionable since the Theileria type Thung Song was located as the intermediate parasite between pathogenic and benign groups. We use the thymidylate synthetase gene (TS) as an alternative for the rapid molecular phylogenetic tree construction of benign Theileria type Thung Song, Theileria sergenti and Theileria buffeli with Babesia bovis as an out-group. The partial nucleotide sequences were determined using PCR, cloning and dideoxy sequencing. The TS nucleotide sequence data were aligned and analyzed by distance and maximum likelihood methods to construct the phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. All tree-building methods gave similar results. This study shows that T. sergenti and T. buffeli are closely related whereas Theileria type Thung Song is more distantly related.

show abstract

Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene variable (V4) region among and within geographic isolates of Theileria from cattle, elk and white-tailed deer

Cited by 60 publications

References 27 publications

Two Theileria Cervi Ssu Rrna Gene Sequence Types Found in Isolates From White-Tailed Deer and Elk in North America

Two Theileria Cervi Ssu Rrna Gene Sequence Types Found in Isolates From White-Tailed Deer and Elk in North America

Non-Transforming Theileria Parasites of Ruminants

Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene

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