Nucleotide sequence and mode of transmission of the wild mouse ecotropic virus, HoMuLV

Voytek, Peter; Kozak, Christine A.

doi:10.1016/0042-6822(89)90221-3

Cited by 19 publications

(21 citation statements)

References 37 publications

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“…Interestingly, the wild mouse virus isolated previously from M. spicilegus, HoMLV, has only one added amino acid residue in this region relative to MoMLV (22). HoMLV is not infectious for M. dunni cells, although it is otherwise ecotropic in host range and (21,22). These results implicate this region of VRA in virus-receptor interactions consistent with previous studies on the structure of the receptor binding site (7).…”

Section: Virus Isolationsupporting

confidence: 80%

“…FrMLV, Rauscher MLV, and AKV ecotropic MLV are all infectious for M. dunni cells (6,14) (Table 2). Interestingly, the wild mouse virus isolated previously from M. spicilegus, HoMLV, has only one added amino acid residue in this region relative to MoMLV (22). HoMLV is not infectious for M. dunni cells, although it is otherwise ecotropic in host range and (21,22).…”

Section: Virus Isolationmentioning

confidence: 99%

“…In addition to its unusual proviral content, M. spicilegus also differs from the other European mice in that it is the only one from which infectious virus has been isolated (21). This isolate is a novel ecotropic virus that was transmitted as an infectious agent in randomly bred laboratory colonies but was lost from these mice sometime after 1985 (22).…”

Section: Spicilegus (Formerly M Hortulanus) Inhabits Central Andmentioning

confidence: 99%

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Generation of Novel Syncytium-Inducing and Host Range Variants of Ecotropic Moloney Murine Leukemia Virus in Mus spicilegus

Jung

Kozak

2003

J Virol

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Mus spicilegus is an Eastern European wild mouse species that has previously been reported to harbor an unusual infectious ecotropic murine leukemia virus (MLV) and proviral envelope genes of a novel MLV subgroup. In the present study, M. spicilegus neonates were inoculated with Moloney ecotropic MLV (MoMLV). All 17 inoculated mice produced infectious ecotropic virus after 8 to 14 weeks, and two unusual phenotypes distinguished the isolates from MoMLV. Studies on virus-host interactions that affect disease induction by the murine leukemia viruses (MLVs) have made extensive use of the inbred laboratory mouse strains with high levels of endogenous virus expression and a high incidence of disease such as AKR and BXH2. Such studies have been highly productive, but use of these laboratory strains provides only limited insight into host response to infection. This is because the common laboratory strains were derived from a small number of progenitors and therefore do not reflect the full range of genetic diversity within Mus (3). Also, the high-virus-titer strains typically used for such studies were bred for their susceptibility to infection and disease and therefore lack the genetic resistance factors that protected their wild mouse progenitors. For example, none of the common inbred strains carry the ecotropic MLV resistance gene Fv4 (10). Also, the major laboratory mouse alleles of the resistance gene, Fv1, Fv1 n and Fv1 b , have not been identified among wild mouse species (12). For these reasons, we believe that it is worthwhile to examine virus susceptibility and the disease process in wild mouse species. Many of these species have experienced long-term exposure to natural MLV infections, have become adapted to endemic infection, and have evolved protective mechanisms that prevent virus-induced mutation and disease. In this report, we describe studies initiated to examine the susceptibility of one wild mouse species, Mus spicilegus, to exogenous infection. M. spicilegus (formerly M. hortulanus) inhabits Central and Eastern Europe and Asiatic Russia, overlapping with M. macedonicus and M. mus musculus (16).M. spicilegus is similar to these two species morphologically and genetically, but not behaviorally, and there is no evidence that interbreeding occurs. M. spicilegus is unusual in its monogamous mating system and in its habit of constructing large mounds of earth and seeds. Several previous studies have examined M. spicilegus for endogenous and infectious MLVs. M. spicilegus differs from the laboratory strains and other European species in that it does not contain endogenous viral env genes related to MCF and xenotropic MLVs (13). Tomonaga and Coffin (20) more recently demonstrated that these mice carry proviral env sequences of a novel subgroup that is equidistant from ecotropic and nonecotropic viruses and that likely represents an ancestral form of MLV. In addition to its unusual proviral content, M. spicilegus also differs from the other European mice in that it is the only one from which infectious viru...

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Section: Virus Isolationsupporting

confidence: 80%

Section: Virus Isolationmentioning

confidence: 99%

Section: Spicilegus (Formerly M Hortulanus) Inhabits Central Andmentioning

confidence: 99%

See 1 more Smart Citation

Generation of Novel Syncytium-Inducing and Host Range Variants of Ecotropic Moloney Murine Leukemia Virus in Mus spicilegus

Jung

Kozak

2003

J Virol

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“…Sources of inbred mouse strains, wild-caught and wild-derived mice, and mouse DNAs were described previously (23). Briefly, DNAs were isolated or obtained from mice maintained in our laboratory or from the randomly bred colonies of M. Potter (National Cancer Institute, Bethesda, MD), The Jackson Laboratory (Bar Harbor, ME), S. Rasheed (University of Southern California, Los Angeles, CA), R. Abe (Naval Medical Research Institute, Bethesda, MD), S. Chattopadhyay and H. Morse III (NIAID, Bethesda, MD), and RIKEN BioResource Center (Ibaraki, Japan), which participates in the National Bio-Resources (28). The primers in Table S1.1 in the supplemental material generated overlapping PCR products that were cloned into pCR2.1-TOPO and sequenced.…”

Section: Virusesmentioning

confidence: 99%

“…The laboratory mouse AKV E-ERV subtype is found in Japan and northwest Asia, and Cas subtype E-ERVs are found in wild mice found in Japan through Southeast Asia and China to Iran as well as California (22,26). The HoMuLV E-MLV and A-MLV are found only in small localized mouse subpopulations in Eastern Europe and southern California, respectively, and have not endogenized in their hosts (27,28). House mice are not native to the Americas but largely derive from animals introduced from Western Europe, M. m. domesticus.…”

mentioning

confidence: 99%

Sequence Diversity, Intersubgroup Relationships, and Origins of the Mouse Leukemia Gammaretroviruses of Laboratory and Wild Mice

Bamunusinghe

Naghashfar

Buckler-White

et al. 2016

J Virol

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Mouse leukemia viruses (MLVs) are found in the common inbred strains of laboratory mice and in the house mouse subspecies of Mus musculus. Receptor usage and envelope (env) sequence variation define three MLV host range subgroups in laboratory mice: ecotropic, polytropic, and xenotropic MLVs (E-, P-, and X-MLVs, respectively). These exogenous MLVs derive from endogenous retroviruses (ERVs) that were acquired by the wild mouse progenitors of laboratory mice about 1 million years ago. We analyzed the genomes of seven MLVs isolated from Eurasian and American wild mice and three previously sequenced MLVs to describe their relationships and identify their possible ERV progenitors. The phylogenetic tree based on the receptor-determining regions of env produced expected host range clusters, but these clusters are not maintained in trees generated from other virus regions. Colinear alignments of the viral genomes identified segmental homologies to ERVs of different host range subgroups. Six MLVs show close relationships to a small xenotropic ERV subgroup largely confined to the inbred mouse Y chromosome. env variations define three E-MLV subtypes, one of which carries duplications of various sizes, sequences, and locations in the proline-rich region of env. Outside the env region, all E-MLVs are related to different nonecotropic MLVs. These results document the diversity in gammaretroviruses isolated from globally distributed Mus subspecies, provide insight into their origins and relationships, and indicate that recombination has had an important role in the evolution of these mutagenic and pathogenic agents. IMPORTANCELaboratory mice carry mouse leukemia viruses (MLVs) of three host range groups which were acquired from their wild mouse progenitors. We sequenced the complete genomes of seven infectious MLVs isolated from geographically separated Eurasian and American wild mice and compared them with endogenous germ line retroviruses (ERVs) acquired early in house mouse evolution. We did this because the laboratory mouse viruses derive directly from specific ERVs or arise by recombination between different ERVs. The six distinctively different wild mouse viruses appear to be recombinants, often involving different host range subgroups, and most are related to a distinctive, largely Y-chromosome-linked MLV ERV subtype. MLVs with ecotropic host ranges show the greatest variability with extensive inter-and intrasubtype envelope differences and with homologies to other host range subgroups outside the envelope. The sequence diversity among these wild mouse isolates helps define their relationships and origins and emphasizes the importance of recombination in their evolution. Mouse leukemia viruses (MLVs) with ecotropic, xenotropic, and polytropic host ranges (E-MLVs, X-MLVs, and P-MLVs, respectively) are readily isolated from the classical strains of the laboratory mouse (reviewed in reference 1). E-MLVs can infect murine cells but not cells of heterologous species; these viruses use the amino acid transporter, CAT-...

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Phylogenetic Relationship of the Complete Rauscher Murine Leukemia Virus Genome with Other Murine Leukemia Virus Genomes

et al. 1997

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We report the complete nucleotide sequence of the genome of Rauscher murine leukemia virus (R-MuLV), the replication-competent helper virus present in the Rauscher virus complex, and its phylogenetic relationship with other murine leukemia virus genomes. An overall sequence identity of 97.6% was found between R-MuLV and the Friend helper virus (F-MuLV), and the two viruses were closely related on the phylogenetic trees constructed from either gag, pol, or env sequences. Moloney murine leukemia virus (Mo-MuLV) was the next closest relative to R-MuLV and F-MuLV on all trees, followed by Akv and radiation leukemia virus (RadLV). The most distantly related helper virus was Hortulanus murine leukemia virus (Ho-MuLV). Interestingly, Cas-Br-E branched with Mo-MuLV on the gag and pol trees, whereas on the env tree, it revealed the highest degree of relatedness to Ho-MuLV, possibly due to an ancient recombination with an Ho-MuLV ancestor. In summary, a phylogenetic analysis involving various MuLVs has been performed, in which the postulated close relationship between R-MuLV and F-MuLV has been confirmed, consistent with the pathobiology of the two viruses.

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Nucleotide sequence and mode of transmission of the wild mouse ecotropic virus, HoMuLV

Cited by 19 publications

References 37 publications

Generation of Novel Syncytium-Inducing and Host Range Variants of Ecotropic Moloney Murine Leukemia Virus in Mus spicilegus

Generation of Novel Syncytium-Inducing and Host Range Variants of Ecotropic Moloney Murine Leukemia Virus in Mus spicilegus

Sequence Diversity, Intersubgroup Relationships, and Origins of the Mouse Leukemia Gammaretroviruses of Laboratory and Wild Mice

Phylogenetic Relationship of the Complete Rauscher Murine Leukemia Virus Genome with Other Murine Leukemia Virus Genomes

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