Nucleotide sequence and genome organization of carnation mottle virus RNA

Abstract

(1,2). This isometric virus infects a wide range of host plants and is one of the simplest of all eukaryotic infectious agents with regard to genome complexity. The smal 1 genome size renders CarMV highly accessible to molecular genetic analysis; thus, CarMV may serve as a useful model to understand RNA plant virus genome structure, function, and regulation.The structure and coding properties of CarMV RNA have been subjects of recent study. In addition to genome-length RNA, two major subgenomic species are produced in vivo and encapsidated in virions (3). Both molecules (previously estimated to have lengths of 1.6 and 1.75 kb) are derived from, and apparently colinear with, the 3' end of the viral genome. Contrary to an earlier study using a wheat-germ extract translation system (4) the small subgenomic RNA exhibits potent messenger activity for production of capsid protein (38,000 molecular weight) in reticulocyte lysates (5,6). The role of the 1.75 kb RNA remains unclear, although it may possess weak messenger activity for coat protein production. Like several other plant viruses, the coat protein gene remains silent, or nearly silent, on full-length genomic © I R L Press Limited, Oxford, England.

The genomic sequence of cardamine chlorotic fleck carmovirus

“…There is another small ORF, encoding the p9 protein, which overlaps the p80RF and the start of the p38 ORF but is in the same reading frame as the replicase. In TCV, it has recently been shown that these proteins are involved in virus movement as previously suggested for TCV and CarMV (Guilley et al, 1985;Carrington et al, 1989;Hacker et al, 1992).…”

“…The genomic sequence of CCFV-CL was translated and compared with those of TCV, carnation mottle (CarMV), and melon necrotic spot (MNSV) carmoviruses (Carrington et al, 1989;Guilley et al, 1985;Riviere & Rochon, 1990) using the Staden (1982), SEQ and GCG programs in a VAX 3100 computer.…”

Nucleotide sequence and genomic organization of melon necrotic spot virus

“…No obvious sequence similarities that might suggest the function of MNSV p7A were found in the databases searched. It has been suggested that CarMV p7 and TCV p8 may be involved in virus transport (Guilley et al, 1985;Carrington et al, 1989).…”

The complete nucleotide sequence of tobacco necrosis virus strain D

“…Fig. 3 shows a comparison between the translation strategies of TNV-D, TNV-A (Meulewaeter et al, 1990), carnation mottle carmovirus (CarMV; Guilley et al, 1985) and cucumber necrosis tombusvirus (CNV; Rochon & Tremaine, 1989). In TNV-A, the p8 and p60RFs are in the same reading frame whereas in TNV-D, the p7a and p7b (shown as a stippled box) ORFs are out of frame but are shown together for comparative purposes and simplicity.…”

“…The genomic organization of TNV-D as compared with that of CNV (Rochon & Tremaine, 1989), CarMV (Guilley et al, 1985) and TNV-A (Meulewaeter et al, 1990) is shown in Fig. 3.…”