The complete nucleotide sequence of tobacco necrosis virus strain D

Coutts, Robert H.A.; Rigden, Justin E.; Slabas, Anthony R.; Lomonossoff, George P.; Wise, P. J.

doi:10.1099/0022-1317-72-7-1521

Cited by 75 publications

(64 citation statements)

References 52 publications

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“…The sequence of the ST9 isolate of BWYV is from Chin et al (1992), that of TNV-D is from Coutts et al (1991). All other sequences were derived from the GenBank and EMBL databases.…”

Section: Analysis Of the 65k Orfmentioning

confidence: 99%

The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA 2

Demler

Rucker

Zoeten

1993

Journal of General Virology

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The genome of pea enation mosaic virus (PEMV) consists of two plus-sense RNAs, both of which are required for mechanical transmission. RNA 1 (5706 nucleotides) has strong sequence similarity with members of the luteovirus group, a similarity that is also manifested in the symptomatology, cytopathology and vector transmission of this virus. RNA 2 (4253 nucleotides) is hypothesized to facilitate systemic invasion and mechanical transmission, attributes that distinguish PEMV from the phloem-limited luteoviruses. Sequence analysis of RNA 2 has demonstrated that PEMV is unique among multicomponent viruses in that it lacks Tand 5'-terminal homology between its genomic RNAs. Sequence analysis of RNA 2 has identified an open reading frame encoding a putative product of 65K that contains a series of polymerase-like motifs typical of viral RNA-dependent RNA polymerases. This protein sequence lacks homology with the polymerase encoded on RNA 1 of PEMV, instead being more closely affiliated with the polymerases of viruses related to the carmoand tombusvirus groups. Inoculation of pea protoplasts with RNA transcripts derived from a full-length cDNA clone ofRNA 2 has demonstrated that RNA 2 replicates autonomously in the absence of RNA 1, although comparable inoculation of whole plants failed to establish a systemic infection. There is no evidence that RNA 2 encodes structural proteins, suggesting that encapsidation functions are supplied in trans by RNA 1, comparable to the helper-dependent complexes occurring within the luteovirus group. These data suggest that the PEMV genome can be characterized as a symbiotic association of two taxonomically distinct viral RNAs cooperatively interacting in the establishment of a systemic virus infection.

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“…The sequence of the ST9 isolate of BWYV is from Chin et al (1992), that of TNV-D is from Coutts et al (1991). All other sequences were derived from the GenBank and EMBL databases.…”

Section: Analysis Of the 65k Orfmentioning

confidence: 99%

The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA 2

Demler

Rucker

Zoeten

1993

Journal of General Virology

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“…Table 1. Percentage nucleotide identity, amino acid identity and similarity (in parentheses) between SCV and related viruses Additional abbreviations and sources for sequences used : AMCV, artichoke mottle crinkle virus (Tavazza et al, 1994) ; BYDV, barley yellow dwarf virus (Miller et al, 1988) ; CNV, cucumber necrosis virus (CNV) (Rochon & Tremaine, 1989) ; CRSV, cymbidium ringspot virus (Dalmay et al, 1993) ; MCMV, maize chlorotic mottle virus (Nutter et al, 1989) ; RCNMV, red clover necrotic mosaic virus (Xiong & Lommel, 1989) ; SBMV, southern bean mosaic virus (Wu et al, 1987) ; TBSV, tomato bushy stunt virus (Hearne et al, 1990) ; TNV-A and -D, tobacco necrosis virus strain A (Meulewaeter et al, 1990) and strain D (Coutts et al, 1991).…”

Section: Comparison Of Scv Nucleotide Sequence With Related Virusesmentioning

confidence: 99%

Genome organization and gene expression of saguaro cactus carmovirus.

Weng

Zhou

1997

Journal of General Virology

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The complete sequence of the single-stranded, (M )-sense RNA genome of saguaro cactus carmovirus (SCV) has been determined. The 3879 nucleotide genome contains five open reading frames (ORFs). The 5h-proximal ORF encodes a 26 kDa protein (p26) and terminates with an amber codon which is readthrough into an in-frame p57 ORF to generate an 86 kDa fusion protein (p86). Two small, centrally located ORFs encode a 6 kDa protein (p6) and a 9 kDa protein (p9), respectively. The 3h-proximal ORF encodes a 37 kDa (p37) capsid protein (CP). Analysis of the nucleotide and predicted amino acid sequences supports the classification of SCV in the genus Carmovirus in the family Tombusviridae. All predicted SCV proteins are expressed in an in vitro

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“…Necroviruses lack the 'P' domain [6,28]. For OLV-1 CP, only the 'S' domain has been identified, covering positions 53 to 270 [11].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the coat protein of olive latent virus 1 isolates

et al. 2013

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The CP gene variability among 21 olive latent virus 1 (OLV-1) isolates obtained from different hosts and locations and at different times was assessed. Amplicons obtained by RT-PCR were cloned, and at least 10 sequences from each isolate were analyzed and compared. OLV-1 sequences available in GenBank were included. The encoded CPs consisted of 270 amino acids, except those of isolates G1S and C7 (269 aa) and G6 (271 aa). Comparison of CP genomic sequences of the isolates under study showed very low values of nucleotide diversity, 0.02, and maximum nucleotide distances between (0.087) or within isolates (0.001). Although very few nucleotide sequence differences were observed among the isolates, olive isolates exhibited lower diversity (0.012). In addition, at position 158 (157 in C7 and G1S and 159 in

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The complete nucleotide sequence of tobacco necrosis virus strain D

Cited by 75 publications

References 52 publications

The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA 2

The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA 2

Genome organization and gene expression of saguaro cactus carmovirus.

Genetic diversity of the coat protein of olive latent virus 1 isolates

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