Nucleotide sequence and genome organization of foot-and-mouth disease virus

Forss, Sonja; Strebel, Klaus; Beck, Ewald; Schaller, Heinz

doi:10.1093/nar/12.16.6587

Cited by 280 publications

(171 citation statements)

References 27 publications

(19 reference statements)

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“…Identity was calculated as a percentage from the output of the SEQAP program using the shorter sequence length as the denominator. Amino acid sequences representing the L protein (Forss et al, 1984;Palmenberg et al, t984), or proteins VP4 to VP1 and 3B to 3D of AGM-27, HAV strain HM-175, encephalomyocarditis virus (Palmenberg et al, 1984), poliovirus type 1 Mahoney (Kitamura et al, 1981 ;Racaniello et al, 1981), foot-and-mouth disease virus type A 12 (Forss et al, 1984), and rhinovirus type 2 (Skein et al, 1985) and type 14 (Stanway et al, 1984;Callahan et al, 1985) were compared using the program RELATE (Dayhoff, 1978). 5" VP4 VP2 VP3 VPI 2A 2B i II I I I I I 735 g04 1470 2208 3108 36753996 2C 3A 3B 3C I II I 5001 5292 Nucleotide numbering is based on the genomic map of HM-175 (Cohen et al, 1987a).…”

Section: Methodsmentioning

confidence: 99%

Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Tsarev

Emerson

Balayan

et al. 1991

Journal of General Virology

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Fragments of eDNA representing greater than 99 % of the entire genome of wild-type hepatitis A virus (HAV) strain AGM-27, isolated from an African green monkey, were obtained by the polymerase chain reaction and sequenced. Comparison with other HAV isolates revealed differences in the predicted amino acid sequence in functionally critical parts of the genome. Comparison of the biological properties of r AGM-27 with those of human wild-type and cell culture-adapted HM-175 strains revealed that AGM-27 grew in cell culture significantly better than did wildtype HM-175, but not as well as cell culture-adapted HM-175. AGM-27 and cell culture-adapted HM-175 were distinguishable by their differential growth in CV-I, FRhK-4 and primary AGMK cells.

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Section: Methodsmentioning

confidence: 99%

Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Tsarev

Emerson

Balayan

et al. 1991

Journal of General Virology

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“…It has recently been reported for the O1 serotype (Forss et al, 1984) that the first AUG downstream from the poly(C) tract could potentially initiate a 10K protein. Strikingly, this particular AUG codon is preceded by a long stretch of pyrimidine residues, a feature which appears to be characteristic of functional initiation sites not only in FMDV ( Fig.…”

Section: Ratios O F the Initiating Proteins Are Reflected By The Surrmentioning

confidence: 99%

“…Indeed, Forss et al (1984) have shown for the 0, serotype of FMDV that eight additional AUGs are located between the poly(C) tract and the initiation sites. Furthermore, we have recently determined the sequence of that portion of the genome to the 5' side of the poly(C) tract and three additional AUG codons have been identified (S. E. Newton, unpublished).…”

Section: B E C L a R K E A N D Othersmentioning

confidence: 99%

Two Initiation Sites for Foot-and-Mouth Disease Virus Polyprotein in vivo

Clarke¹,

Sangar²,

Burroughs

et al. 1985

Journal of General Virology

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SUMMARYTypically, the translation of eukaryotic mRNAs into protein is initiated at a single site. However, we have recently shown that not one but two primary products, P20a and P I6, are translated from the 5' end of the coding region of the genome of foot-andmouth disease virus (FMDV). In this paper we show by partial protease digestion of these proteins that they differ only at their N termini, thus confirming the presence of two initiation sites for translation of FMDV RNA. Sequence analysis of two subtypes of the virus (Al0 and A12) confirms the presence of two initiator AUG codons in the expected position on the genome. By correlation with protein synthesis data from these subtypes it appears that the relative use of each initiation site is dependent on its surrounding nucleotide sequence. In addition, the ratio of the two proteins when synthesized in vitro differs markedly from that when they are synthesized in vivo, suggesting the presence of a control mechanism for synthesis of P20a in vivo which may be absent in vitro. We also show that the cleavage site between these two proteins and the structural protein precursor, P88, is located closer to the N terminus of the polyprotein than has previously been reported.

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“…The genome is of 8.5 kb long ssRNA of positive sense. The genome carries single open reading frame resulting in translation of a large polypeptide, which undergoes proteolytic cleavage to form mature viral proteins [14].…”

Section: Introductionmentioning

confidence: 99%

Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

et al. 2011

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DNA vaccines are considered as alternatives to live attenuated ones for those diseases like foot-and-mouth disease (FMD) where the production and application of live vaccines have been found unsuccessful. However, stability of DNA and the quantity of antigen expressed are the major limitation with naked DNA vaccines. To address these issues self replicating gene vaccine construct was made for foot-and-mouth disease virus (FMDV) type 'O' and studied. The vector for vaccine construct, designated as pSinCMVVac carried CMV promoter and Poly(A) signal sequences at 5 0 and 3 0 end of Sindbis replicase gene respectively. Gene for structural protein precursor (P1-2A) of FMDV serotype 'O' was inserted into pSinCMVVac under subgenomic promoter. 5 0 UTR (untranslated region) of FMDV was introduced upstream of P1-2A to enhance the level of expression of cloned gene. Functionality of the vaccine construct was confirmed in vitro and in vivo. The self-replicating gene vaccine construct was tested in cattle in comparison with naked DNA vaccine carrying P1-2A and 3CD (pUP3CD). Humoral immune response by ELISA and SNT and cellular response by lymphoproliferation assay using MTT were studied. The default approach of using self replicating gene vaccine in high dose and multiple injection in cattle as followed in our studies might result in immunosuppression as this was observed in our subsequent experiments in guinea pigs. Hence based on dose response studies, vaccine strategy needs to be decided. However, the approach of using Sindbis polymerase gene and UTR in FMDV vaccine is the first report and shows future scope of developing such vaccines.

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Nucleotide sequence and genome organization of foot-and-mouth disease virus

Cited by 280 publications

References 27 publications

Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Two Initiation Sites for Foot-and-Mouth Disease Virus Polyprotein in vivo

Self Replicating Gene Vaccine Carrying P1-2A Gene of FMDV Serotype O and its Effects on the Immune Responses of Cattle

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