Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Tsarev, Sergei A.; Emerson, Suzanne U.; Balayan, M. S.; Ticehurst, John R.; Purcell, Robert H.

doi:10.1099/0022-1317-72-7-1677

Cited by 73 publications

(56 citation statements)

References 30 publications

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“…Finally, when our isolate was compared with the AGM-27 strain, a nucleotide homology of 81.2% was observed. These results confirm previous studies in which the AGM-27 strain proved to be unique, because it differs significantly from any other HAV isolate , Tsarev et al 1991, such as the CF53/Berne, SLF88 and NOR-21 isolates (Ching et al 2002, Lu et al 2004, Stene-Johasen et al 2005). …”

Section: Genetic Relationship Between Haf-203 and Othersupporting

confidence: 81%

“…(Hu et al 2002); LA (Najarian et al 1985); HAS-15 (Sverlov et al 1987); M2 (AY974170, Badillo et al unpublished); HAV-FG (Beneduce et al 1995); GBM (Graft et al 1994(Graft et al , 1997; MBB (Paul et al 1987); IVA (DQ646426, Bitchenco et al unpublished) HM-175, HM-175 attenuated and NC001489 (Cohen et al 1987a,b); CF53/Berne (Lu et al 2004); SLF88 (Ching et al 2002 ) ;NOR-21 (Stene-Johansen et al 2005); AGM-27 (Tsarev et al 1991). respectively), in the 2B protein (nucleotide 3880 was altered from T→C) and in the 3D protein (nucleotide 6232 changed from T→C and nucleotide 7206 from A→G). Mutations were also witnessed in 5'and 3' untranslated regions (UTRs).…”

Section: Resultsmentioning

confidence: 99%

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Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Baptista¹,

Silva²,

Lima³

et al. 2006

Mem. Inst. Oswaldo Cruz

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The hepatitis A virus (HAV) HAF-203 strain was isolated from an acute case of HAV infection. The primary isolation of HAF-203 in Brazil and its adaptation to the FRhK-4 cell lineage allowed the production of large amounts of viral particles enabling molecular characterization of the first HAV isolate in Brazil. The aim of our study was to determine the nucleotide sequence of the HAF-203 strain genome, compare it to other HAV genomes and highlight its genetic variability. The complete nucleotide sequence of the HAF-203 strain (7472 nucleotides) was compared to those obtained earlier by others for other HAV isolates. These analyses revealed 19 HAF-specific nucleotide sequence differences with 10 amino acid substitutions. Most of the non-conservative changes were located at VP1, 2C, and 3D genes, but the 3B region was the most variable. The availability of HAF-203 complementary DNA was useful for the production of the recombinant VP1 protein, which is a major determinant of viral infectivity. This recombinant protein was shown by enzyme-linked immunoassay and blotting, to be immunogenic and resemble the native protein, therefore suggesting its value as a reagent for incorporation into diagnostic tests. Key words: hepatitis A virus (HAV) -HAF-203 strain -complete genome (Brazil) -VP1 gene recombinant proteinEscherichia coli expression -HAV diagnosisThe hepatitis A virus (HAV) is the causative agent of an old and common human disease, with high incidence rates, still present in many countries throughout the world. In Brazil, this disease has been responsible for 50% of the acute hepatitis cases referred to the National Reference Center for Viral Hepatitis/Oswaldo Cruz Institute/Ministry of Health. The etiological agent was first identified by Feinstone et al. (1973), and the virus is currently classified as the first and unique member of the genus Hepatovirus, Picornaviridae family (Minor 1991).HAV is a 27-nm non-enveloped icosahedral particle, which comprises a single stranded plus-sense RNA (Coulepis et al. 1981). The viral genome is composed of approximately 7500 nucleotides, edged by a covalently linked VPg protein at the 5' terminal and a polyadenylated 3' end. The viral RNA encodes a single polyprotein of 2224 amino acids, which is later cleaved into 11 viral polypeptides (Cohen et al. 19847a).The Brazilian HAF-203 strain investigated here was obtained from a faecal specimen of an acute hepatitis A patient. The viral isolation and serial passage history was previous reported by Gaspar et al. (1992). The HAF-203 HAV strain was inoculated into fetal Rhesus kidney cells (Baptista ML et al. unpublished). In the present study, we have sequenced the complete genome of the HAF-203 HAV strain and compared it with other HAV isolates. Several groups have adopted recombinant viral proteins for diagnostic purposes (Ostermayer et al. 1987, Johnston et al. 1988, LaBrecque et al. 1998, Ternovai et al. 2001, Di Napoli et al. 2004. A fragment of the HAF-203 strain encoding the complete VP1 gene was cloned and expressed in E...

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Section: Genetic Relationship Between Haf-203 and Othersupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Baptista¹,

Silva²,

Lima³

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…In addition, there is no evidence of human disease due to the transmission of these viruses to animal handlers. These three simian strains also have important amino acid substitutions Tsarev et al, 1991) within the immunodominant antigenic site of HAV (Ping et aL, 1988), and thus they may represent serological variants of HAV. On the other hand, there is no obvious difference in disease expression between genotype I and III viruses.…”

Section: Discussionmentioning

confidence: 99%

“…Table 1. The extent of nucleotide variation among these 152 strains within a 168 base region encoding the putative VPI/2A junction provided a measure of the relatedness of individual human strains to each other, and to recently characterized simian HAV strains Tsarev et al, 1991). A pairwise analysis of the nucleotide sequences of cDNA amplified by a PCR-based procedure resulted in the dendrogram shown in Fig.…”

Section: Genetic Analysis Of Wild-type Ha V Strainsmentioning

confidence: 99%

Genetic relatedness of hepatitis A virus strains recovered from different geographical regions

Robertson

Jansen

Khanna

et al. 1992

Journal of General Virology

439

519

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A pairwise comparison of the nucleic acid sequence of 168 bases from 152 wild-type or unique cell cultureadapted strains of hepatitis A virus (HAV) revealed that HAV strains can be differentiated genetically into seven unique genotypes (I to VII). In general, the nucleotide sequence of viruses in different genotypes differs at 15 to 25 % of positions within this segment of the genome. Viruses from four of the genotypes (I, II, III and VII) were recovered from cases of hepatitis A in humans, whereas viruses from the other three genotypes (IV, V and VI) were isolated only from simian species developing a hepatitis A-like illness during captivity. Among non-epidemiologically related human HAV strains, 81 were characterized as genotype I, and 19 as genotype III. Within each of these major genotypes, there were two distinct groups (subgenotypes), which differed in sequence at approximately 7-5 % of base positions. Each genotype and subgenotype has a characteristic amino acid sequence in this region of the polyprotein, with the most divergent genotypes differing at 10 of 56 residues. Strains recovered from some geographical regions belonged to a common (endemic) genotype, whereas strains from other regions belonged to several, probably imported, genotypes. Thus, HAV strains recovered in North America were for the most part closely related at the nucleotide sequence level, whereas in other regions, such as Japan and Western Europe, HAV strains were derived from multiple genotypes or sub-genotypes. These data indicate that patterns of endemic transmission can be differentiated from situations in which infections are imported due to travel.

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“…Cabe aclarar que el genotipo III es el único grupo que incluye aislamientos de humanos y de primates. Los aislamientos de simios pueden diferenciarse por un polimorfismo característico en la unión VP3-VP1 que no poseen las cepas aisladas de humanos (54)(55)(56).…”

Section: Genotipos Del Vhaunclassified

Infección por el virus de la hepatitis A: epidemiología y diversidad genética

Navas

Triana

2015

iatreia

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RESUMENLa infección por el virus de la hepatitis A es un problema global de salud pública. El virus está ampliamente distribuido y es la principal causa de hepatitis aguda de transmisión entérica en Latinoamérica. La partícula viral es estable en el medio ambiente y conserva su infectividad por varias semanas, lo que facilita su transmisión por agua y alimentos contaminados. Mundialmente se han descrito distintos patrones epidemiológicos, que pueden cambiar en el tiempo al modificarse variables socioeconómicas en la población como las condiciones sanitarias básicas y la vacunación. Esto deja al descubierto nuevas poblaciones susceptibles a la infección. En Latinoamérica se ha descrito la circulación del genotipo I y los subgenotipos A y B, pero se requieren más investigaciones que aporten el conocimiento necesario para gestionar planes de prevención y control para la disminución mundial de la prevalencia de la infección. Para este artículo se hizo una revisión de la literatura en las bases de datos SciELO, PubMed y ScienceDirect bajo los términos de búsqueda "Virus de la hepatitis A", "Epidemiología", "Seroprevalencia" e "Infección". De los resultados obtenidos se incluyeron únicamente las publicaciones en inglés y español que describieran estudios epidemiológicos y moleculares de interés en Latinoamérica. PALABRAS CLAVEEpidemiología; Hepatitis; Salud Pública; Virus de la Hepatitis A SUMMARY Hepatitis A virus infection: Epidemiology and genetic diversityHepatitis A virus infection is a global public health problem. The virus has a wide range of distribution and it is the main cause of acute hepatitis transmitted by the enteric route in

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Simian hepatitis A virus (HAV) strain AGM-27: comparison of genome structure and growth in cell culture with other HAV strains

Cited by 73 publications

References 30 publications

Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Genetic variability of hepatitis A virus strain HAF-203 isolated in Brazil and expression of the VP1 gene in Escherichia coli

Genetic relatedness of hepatitis A virus strains recovered from different geographical regions

Infección por el virus de la hepatitis A: epidemiología y diversidad genética

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