Nucleotide sequence and embryonic expression of quail and duck Sox9 genes

Takada, Shuji; Ota, Jun; Kansaku, Norio; Yamashita, Hideji; Izumi, Toshio; Ishikawa, Madoka; Wada, Toyoji; Kaneda, Ruri; Choi, Young Lim; Koinuma, Koji; Fujiwara, Shin-ichiro; Aoki, Hirotaka; Kisanuki, Hiroyuki; Yamashita, Yuichi; Mano, Hiroyuki

doi:10.1016/j.ygcen.2005.08.009

Cited by 26 publications

(17 citation statements)

References 18 publications

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“…Before that age, the sex of the gonads is relatively easily to determine by laparotomy, which yields an accurate result starting at day 9 of incubation (Schumacher et al 1988). Before embryonic day 9, molecular sexing is a reliable method (Takada et al 2006). …”

Section: Studies Of the Neuroendocrine Basis For Hormonal Activation mentioning

confidence: 99%

Japanese Quail as a Model System for Studying the Neuroendocrine Control of Reproductive and Social Behaviors

Ball¹,

Balthazart²

2010

ILAR Journal

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Japanese quail (Coturnix japonica; referred to simply as quail in this article) readily exhibit sexual behavior and related social behaviors in captive conditions and have therefore proven valuable for studies of how early social experience can shape adult mate preference and sexual behavior. Quail have also been used in sexual conditioning studies illustrating how natural stimuli predict successful reproduction via Pavlovian processes. In addition, they have proven to be a good model to study how variation in photoperiod regulates reproduction and how variation in gonadal steroid hormones controls sexual behavior. For example, studies have shown that testosterone activates male-typical behaviors after being metabolized into estrogenic and androgenic metabolites. A critical site of action for these metabolites is the preoptic medial nucleus (POM), which is larger in males than in females. The enzyme aromatase converts testosterone to estradiol and is enriched in the POM in a male-biased fashion. Quail studies were the first to show that this enzyme is regulated both relatively slowly via genomic actions of steroids and more quickly via phosphorylation. With this base of knowledge and the recent cloning of the entire genome of the closely related chicken, quail will be valuable for future studies connecting gene expression to sexual and social behaviors.

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Section: Studies Of the Neuroendocrine Basis For Hormonal Activation mentioning

confidence: 99%

Japanese Quail as a Model System for Studying the Neuroendocrine Control of Reproductive and Social Behaviors

Ball¹,

Balthazart²

2010

ILAR Journal

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“…These similar expression patterns suggest that regulation of Amh expression is conserved among these three avian species. The expression patterns of quail and duck Amh are also similar to those of quail and duck Sox9 (Takada et al, 2006). The earliest detectable stages examined so far for malespecific up-regulation of Amh and Sox9 is same in quail and duck (quail at day 5and duck at day 7), however, there was a difference in expression patterns between Amh and Sox9 in female gonads of quail and duck; Amh is expressed at low levels but Sox9 is not.…”

Section: Cloning and Expression Patterns Of Quail And Duck Amhmentioning

confidence: 58%

“…Sense and antisense oligonucleotides corresponding to this 27-bp sequence were synthesized and used for 3 0 and 5 0 RACE, respectively. Given that sex differentiation occurs before day 5 in quail embryos and day 7 in duck embryos (Takada et al, 2006), we used RNA purified from the gonads of male quail and duck embryos on days 7 and 8, respectively, as a template for RACE. The nucleotide sequences of 5 0 and 3 0 RACE products were determined and assembled to yield the corresponding cDNA sequences.…”

Section: Cloning and Expression Patterns Of Quail And Duck Amhmentioning

confidence: 99%

“…Staging of duck embryos was compared at dissection with chicken stages (Hamburger and Hamilton, 1951). The urogenital ridge of each embryo was explanted for whole-mount in situ hybridization, and the hind limb was used for extraction of genomic DNA and polymerase chain reaction (PCR)-based sexing as described (Clinton et al, 2001;Takada et al, 2006).…”

Section: Animalsmentioning

confidence: 99%

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Evidence for activation of Amh gene expression by steroidogenic factor 1

Takada

Wada

Kaneda

et al. 2006

Mechanisms of Development

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The anti-Müllerian hormone gene (Amh) is responsible for regression in males of the Müllerian ducts. The molecular mechanism of regulation of chicken Amh expression is poorly understood. To investigate the regulation of chicken Amh expression, we have cloned Amh cDNAs from quail and duck as well as the promoter regions of the gene from chicken, quail, and duck. The expression patterns of Amh during embryonic development in these three species were found to be similar, suggesting that the regulatory mechanisms of Amh expression are conserved. The sequence of the proximal promoter of Amh contains a putative binding site for steroidogenic factor 1 (SF1), the protein product of which can up-regulate Amh in mammals. We showed here that SF1 is able to activate the chicken Amh promoter and binds to its putative SF1 binding site. These results suggest that SF1 plays a role in regulation of Amh expression in avian species.

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“…The sex of quail and duck embryos can be identified based on Sox9 gene expression [53]. Sox9 is a member of the Sry-type HMG-box (Sox) gene family.…”

Section: Other Genesmentioning

confidence: 99%

Identification of Sexes in Poultry Using DNA Based Methods

2017

International Journal of Research Studies in Microbiology and B

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Nucleotide sequence and embryonic expression of quail and duck Sox9 genes

Cited by 26 publications

References 18 publications

Japanese Quail as a Model System for Studying the Neuroendocrine Control of Reproductive and Social Behaviors

Japanese Quail as a Model System for Studying the Neuroendocrine Control of Reproductive and Social Behaviors

Evidence for activation of Amh gene expression by steroidogenic factor 1

Identification of Sexes in Poultry Using DNA Based Methods

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