Nucleotide sequence analysis of S-locus genes to unify S haplotype nomenclature in radish

Haseyama, Yurie; Kitashiba, Hiroyasu; Okamoto, Shingo; Tonouchi, Emiko; Sakamoto, Koji; Nishio, Takeshi

doi:10.1007/s11032-018-0874-2

Cited by 14 publications

(44 citation statements)

References 32 publications

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“…Haseyama et al. () suggested RsS‐1 Lim and RsS‐30 may be the same S haplotypes based on highly similar (100% identity) of SLG sequence. However, the nucleotide sequences of the kinase domain of SRK in RsS‐30 were lowly similar (86% identity) to RsS‐1 Lim.…”

Section: Resultsmentioning

confidence: 99%

“…The S domain of SRK sequences in P27 was 99.7% similar to the RsS‐26 (GenBank: ), with 100% coverage and only three nucleotide differences. RsS‐4 Lim and RsS‐26 may refer to the same S haplotype (Haseyama et al., ). Thus, we propose that the S haplotype of lines P27 and P17 was RsS‐26 and RsS‐5 Lim, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Haseyama et al. () suggested the total number of S haplotypes can be estimated to be 35, of which 9 and 11 specific S haplotypes in Japan and Korea, respectively. In the current study, we identified 18 S haplotypes, 10 S haplotypes were found to be same as those in the Japanese radish and the Korean radish, 5 S haplotypes were consistent with that in Korean radish, and only 3 S haplotypes were unique in Chinese radish.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

“…Recently, Haseyama et al. () published 23 different S haplotypes and suggested a unification of these S haplotypes in radish. In the present study, we used the numbering system and identified the S haplotypes of 63 radish inbred lines, mainly from China, by sequencing SLG or SRK and completing BLAST searches.…”

Section: Introductionmentioning

confidence: 99%

“…However, same or similar sequences with different S haplotype names and different sequences with the same S haplotype names have been registered in public databases, which results in confusion of S haplotype names for researchers and breeders (Nishio & Sakamoto, 2017). Recently, Haseyama et al (2018) published 23 different S haplotypes and suggested a unification of these S haplotypes in radish. In the present study, we used the numbering system and identified the S haplotypes of 63 radish inbred lines, mainly from China, by sequencing SLG or SRK and completing BLAST searches.…”

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confidence: 99%

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Identification and classification of S haplotypes in radish (Raphanus sativus)

Wang

Zheng²,

Zhang

2018

Plant Breeding

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Radish (Raphanus sativus L.) is a typical cross‐pollinated crop that exhibits obvious heterosis. Self‐incompatibility is an important character for F1 hybrid breeding of radish. Knowledge of the S haplotypes of breeding lines is very important for breeders to avoid cross‐incompatibility of the parental lines. In the present study, the S haplotypes of 63 radish inbred lines, which were independently cultivated by our research group, were identified by PCR amplification, sequencing and BLAST analyses of the SRK and SLG genes. Finally, fifty‐four inbred lines were classified into 15 class I S haplotypes, including three new types, RsS‐38, RsS‐39 and RsS‐40. Additionally, three class II S haplotypes were identified in nine radish inbred lines. Partial SRK or SLG sequences were completed, such as RsS‐11 Lim (SRK‐S), RsS‐26 (SRK‐K), RsS‐5 Lim (SRK‐K and SRK‐S) and RsS‐9 (SRK‐K). The identified S haplotypes were verified with a cross‐pollination test, and RsS‐9 has weaker self‐incompatibility than other S haplotypes. These information will not only contribute to the production of hybrid seeds but also to the development of new self‐compatible inbred lines, which were advantageous of the production of maintain line and male line in CMS breeding system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Conclusion and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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