Nucleotide Levels in Human and Bovine Lenses: A Study on Regional postmortem Changes

Abstract

Regional lens adenine nucleotide levels were determined in human and bovine lenses using sensitive HPLC techniques. In adult lenses of both species adenine nucleotide levels were highest in the anterior cortex (plus epithelium and capsule) followed by the posterior cortex (plus capsule) and lowest in the nucleus. With increasing lens age ATP level remained steady in the anterior cortex (plus epithelium and capsule) and nucleus, while those in the posterior cortex showed a tendency toward decrease. In both species, human as well as bovine, adenine nucleotide levels of the anterior cortex (plus epithelium and capsule) underwent early postmortem changes. Thus, immediate postmortem sampling as well as regional determinations are a must for detailed studies of lens nucleotide metabolism.

Detection of High-Energy Phosphates in Cortical Bone as an Indicator of Bone Healing and Remodelling: Use of a Rabbit Model

“…To avoid the rapid degradation of the cortical phosphates, all samples were immediately frozen in liquid nitrogen (-196 Ο C). 7 Both wet and dry masses were measured to eliminate systematic errors: Idström et al 8 reported that the tissue's wet mass increases in ischaemic tissue because of metabolite accumulation and disturbances in tissue perfusion. Each specimen was lyophilised for 36 hours (Lyovac GT 2, W. C. Heraeus, GmbH & Co., Hanau, Germany) at -80 Ο C in order to prevent degradation.…”

“…Each specimen was lyophilised for 36 hours (Lyovac GT 2, W. C. Heraeus, GmbH & Co., Hanau, Germany) at -80 Ο C in order to prevent degradation. 7 The lyophilised tissue was then pulverised with a grinder in liquid nitrogen and dispersed in 300 µl perchloric acid. The samples were then centrifuged at 14 000 revolutions per minute for 20 minutes.…”