Nucleotide and Oligonucleotide Compositions of the Alanine-, Valine-, and Tyrosine-Acceptor “Soluble” Ribonucleic Acids of Yeast

The sequence of sequencers: The history of sequencing DNA

“…Furthermore, RNase enzymes able to cut RNA chains at specific sites were already known and available. Despite these advantages, progress remained slow, as the techniques available to researchers – borrowed from analytical chemistry – were only able to measure nucleotide composition, and not order [5] . However, by combining these techniques with selective ribonuclease treatments to produce fully and partially degraded RNA fragments [6] (and incorporating the observation that RNA contained a different nucleotide base [7] ), in 1965 Robert Holley and colleagues were able to produce the first whole nucleic acid sequence, that of alanine tRNA from Saccharomyces cerevisiae [8] .…”

New Approaches to Bacterial Taxonomy

“…The four known classes of RNA include the three cellular varieties, soluble RNA (s-RNA), ribosomal RNA (r-RNA) , and messenger RNA (m-RNA) as well as virus RNA. Soluble RNA, which accounts for approximately 10 per cent of the total RNA, is also rather invariant in its composition (about 50 to 65 per cent GC) (285,287). Ribosomal RNA from different bacteria, wr.ich comprises the bulk of the cellular RNA (about 80 to 85 per cent), does not vary greatly in its base composition (the range is about 50 to 60 per cent GC) (285,286).…”

Metabolism of Nucleic Acids

“…At present. it is difficult to interpret such findings, especially because of the ubiquitous presence of ribonuclease (RNase) (38), but the results are similar to those obtained with the deoxyribonuclease (DNase) of erythrocytes on DNA and described under nucleases. In contrast to this depolymerization of RNA is the polymerizing effect of polyalkylamines on RNA.…”