Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance geneTuRB01

Lehmann, P.; Petrzik, Karel; Jenner, Carol E.; Greenland, Andrew J.; Kozubek, Edward; Walsh, John A.

doi:10.1006/pmpp.1997.0116

Cited by 32 publications

(25 citation statements)

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“…In initial experiments, systemic accumulation of the avirulent TuMV isolate CDN1 (Jenner et al, 2000;Lehmann et al, 1997) in At-eIF(iso)4E KO plants was occasionally observed at low frequency (,5 %) during several independent inoculation experiments. Serial propagation of these viral isolates resulted in the selection of TuMV CDN1 variants able to systemically infect 100 % of inoculated At-eIF(iso)4E plants, as revealed by symptom development and RT-PCR assays (data not shown).…”

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confidence: 99%

Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G

Gallois

Charron

Sánchez

et al. 2009

Journal of General Virology

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Previous resistance analyses of Arabidopsis thaliana mutants knocked out for eukaryotic translation initiation factors showed that disruption of the At-eIF(iso)4E or both the AteIF(iso)4G1 and At-eIF(iso)4G2 genes resulted in resistance against turnip mosaic virus (TuMV). This study selected TuMV virulent variants that overcame this resistance and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in At-eIF(iso)4E and At-eIF(iso)4G1¾At-eIF(iso)4G2 knockout plants. As a VPg-eIF(iso)4E interaction has been shown previously to be critical for TuMV infection, a systematic analysis of the interactions between A. thaliana eIF4Es and VPgs of virulent and avirulent TuMVs was performed. The results suggest that virulent TuMV variants may use an eIF4F-independent pathway.The eukaryotic translation initiation factors eIF4E and eIF4G play a key role during virus infection in plants ( Robaglia & Caranta, 2006). During mRNA translation, eIF4E provides the cap-binding function and is associated with eIF4G, the scaffold for the other components of the translation initiation complex, to form the eIF4F complex (Marcotrigiano et al., 1999). In plants, a second eIF4F complex called eIF(iso)4F is present, and results from the assembly of eIF(iso)4E and eIF(iso)4G isoforms (Browning, 2004). These two complexes perform essentially the same task in translation, but have different affinities for certain classes of mRNA substrates and are probably involved in different cellular events (Gallie & Browning, 2001). Plant genes encoding proteins from the eIF4F and eIF(iso)4F complexes belong to small gene families. In Arabidopsis thaliana, three genes encode the eIF4E subfamily proteins (eIF4E1, eIF4E2 and eIF4E3), one encodes eIF(iso)4E and one a non-canonical eIF4E-like protein, known as novel cap-binding protein (nCBP). A single gene encodes eIF4G, whilst two genes encode the eIF(iso)4G subfamily proteins [eIF(iso)4G1 and eIF(iso)4G2] ( Robaglia & Caranta, 2006). Viruses that depend on these factors to perform their infectious cycle include members of the genus Potyvirus, the largest and the most diverse genus of plant viruses (Adams et al., 2005;Shukla et al., 1994). Potyviruses are characterized by a positive-sense, single-stranded RNA genome of about 10 kb that encodes at least ten multifunctional proteins. The genome is 39-polyadenylated and covalently linked at its 59 end to a virus-encoded protein (VPg) (Murphy et al., 1991;Revers et al., 1999).Potyviruses may selectively use either the eIF4E or eIF(iso)4E isoform in A. thaliana to achieve a successful infection (Sato et al., 2005). We have demonstrated that potyviruses, including turnip mosaic virus (TuMV), selectively use members of the eIF4G family in a fashion that parallels the selective recruitment of eIF4E isoforms (Nicaise et al., 2007;Sato et al., 2005 ; data not shown).In order to confirm that each single amino acid change in the VPg cistron of TuMV determined its virulence on the At...

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confidence: 99%

Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G

Gallois

Charron

Sánchez

et al. 2009

Journal of General Virology

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“…Two oligonucleotide primers flanking the TuMV CP gene sequence were used (P4S, 5'-ATCAAGCTTCAGGCAATCTTTGAGGATT AT-3'; and P3S, 5'-ATCGTCGACTACTAC TCGAGTTTTTTTTTTTTTTTTT-3') during PCR performed as described previously (Lehmann et al 1997). PCR products were analysed on 1.0% agarose gels.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

“…Westar plants with TRIZOL according to the manufacturer's recommendations. RT-PCR was performed as described previously (Lehmann et al 1997). During RT-PCR, primers designed to produce overlapping products of approximately 1-2 kb along the length of the viral genome were used ( Figure 1).…”

Section: Nucleotide Sequencingmentioning

confidence: 99%

“…The RNA of TuMV has a single ORF that is translated into a single large polyprotein, which is digested by viral proteases into a few proteins (Riechmann et al 1992). Several partial nt sequences of different TuMV isolates have been reported (Kong et al 1990;Nakashima et al 1991;Sano et al 1992;Choi and Choi 1993;Nakashima et al 1993;Kim et al 1995;Petrzik and Lehmann 1996;Lehmann et al 1997). The first complete nt sequences of Canadian and Japanese isolates were presented by Nicolas and Lalibertë (1992) and Ohshima et al (1996).…”

Section: Introductionmentioning

confidence: 99%

“…Nucleotide sequence analysis has also been used to classify TuMV isolates. Nucleotide sequences of some fragments of the TuMV genome have been used to classify isolates: the 3'-untranslated region (3'-UTR) (Petrzik and Lehmann 1996), the coat protein (CP) gene (Lehmann et al 1997;Chen et al 2002), or both the P1 and CP genes (Ohshima et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Genetic and molecular variability of aTurnip mosaic virus population from horseradish (Cochlearia armoracia L.)

2007

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Variability and genetic structure of a novel Turnip mosaic virus (TuMV) population from horseradish (Cochlearia armoracia L.) were examined. Over 60 horseradish plants were tested to identify a total of 28 TuMV isolates, constituting the Cochlearia ARmoracia (CAR) TuMV population. Two subgroups of the CAR TuMV isolates could be distinguished: subgroup N did not infect oilseed rape (Brassica napus var. oleifera) cv. Westar plants, while subgroup A infected these plants systemically. Two types of infection of oilseed rape plants were induced by inoculation with the CAR TuMV isolates: systemic mosaic infection and systemic necrotic lesions. The complete sequences of isolates CAR37 (subgroup N) and CAR37A (subgroup A) were determined and compared. The sequences of HC-Pro and CP genes of CAR37 and CAR37A and other isolates of TuMV from other countries were compared to provide some insight into their relatedness. CAR37A, initially regarded as a variant, proved to be very different from CAR37. Re-sequencing after repeated passages confirmed the genetic stability of both isolates.

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Resistance to Turnip mosaic virus in the Brassicaceae

Walsh

Jenner

Natural Resistance Mechanisms of Plants to Viruses

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Nucleotide and amino acid variation in the coat protein coding region of turnip mosaic virus isolates and possible involvement in the interaction with the brassica resistance geneTuRB01

Cited by 32 publications

References 12 publications

Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G

Single amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G

Genetic and molecular variability of aTurnip mosaic virus population from horseradish (Cochlearia armoracia L.)

Resistance to Turnip mosaic virus in the Brassicaceae

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