Nucleoside Triphosphates as Promoters to Enhance Nanoceria Enzyme‐like Activity and for Single‐Nucleotide Polymorphism Typing

Abstract

Nucleoside triphosphates (NTPs) can improve the oxidase‐like activity of nanoceria and the enhancement is correlated with the type of NTP. This effect is demonstrated to be as a result of the coupling of the oxidative reaction with the NTP hydrolysis reactions, as the nanoceria has both oxidase‐like and phosphatase‐like activities. The differences reflect the different dephosphorylation catalytic activities of nanoceria to the NTP used. Furthermore, based on the NTP‐promoted oxidase‐like activity of nanoceria and the differences among the different types of NTPs, series effective and high‐throughput colorimetric assays for single‐nucleotide polymorphism (SNP) typing are developed.

Characterization of glucose oxidation by gold nanoparticles using nanoceria

“…In the past decade, gold nanoparticles (AuNPs) [4][5][6][7], magnetic iron oxide NPs [8], and cerium oxide NPs (nanoceria) have been reported to mimic various enzymes [9][10][11][12][13][14][15][16]. Oxidation of glucose by AuNPs was first reported by Comotti et al in 2004 [4].…”

Cerium oxide nanoparticle: a remarkably versatile rare earth nanomaterial for biological applications

“…Qu and colleagues 71 reported that nucleoside triphosphates (NTPs) can improve the oxidase-like activity of CeONP and that the enhancement is correlated with the type of NTPs ( Figure 5C). This effect was demonstrated to be a result of the coupling of the oxidative reaction with the NTPs' hydrolysis reactions, because CeONP has both oxidase-like and phosphatase-like activities.…”

Boosting the oxidase mimicking activity of nanoceria by fluoride capping: rivaling protein enzymes and ultrasensitive F−detection

“…9,[19][20][21][22][23] The best known examples are gold nanoparticles for glucose oxidation, 9 and CeO2 nanoparticles (nanoceria) that oxidize a diverse range of substrates. 20 Oxidase nanozymes are important since they do not require unstable H2O2 as a co-substrate.Nanoceria is a versatile nanozyme, displaying oxidase, 16,20 catalase, 24,25 superoxide dismutase, 26,27 and phosphatase 28 activities under different conditions. In 2009, Asati and co-workers first reported the oxidase-like activity of nanoceria.…”

“…The catalytic activities of nanoceria were attributed to the mixed oxidation states of Ce 3+ and Ce 4+ , and related oxygen vacancies. 29,30 The role of redox states, 24 surface coating, 20 pH, 31 buffer composition, 32 and nucleotide triphosphate 16 were investigated. We hypothesize that surface chemistry plays a critical role in modulating substrate/product adsorption and electron transfer, thus influencing catalytic efficiency.…”