Characterization of glucose oxidation by gold nanoparticles using nanoceria

Lang, Nathan J.; Liu, Biwu; Liu, Juewen

doi:10.1016/j.jcis.2014.04.025

Cited by 90 publications

(67 citation statements)

References 33 publications

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“…In the biological applications such as target drug delivery, biosensing, and bioimaging, it is necessary to graft nanozymes with affinity ligands. However, nanozymes might loss catalytic activity if their surface is densely capped since the reaction might take place on the surface of nanozymes (e.g., oxidase‐like activity) . Because of the complexity of surface, so far there is no universal way to achieve bioconjugation.…”

Section: The Structure Of Nanozymesmentioning

confidence: 99%

Recent Advances in Nanozyme Research

2018

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inflammatory response to combat infections and can also react with many important biomolecules including proteins, lipids, and nucleic acid polymers (RNA and DNA). [7] Low dosage of ROS plays important roles in signal transduction, cell proliferation/migration/differentiation, and body defense against pathogen invasion. However, abnormally elevated ROS level can destroy redox homeostasis and cause oxidative stress and serious damage of structure and function of macromolecules in the cell. Research during the last decade has shown that oxidative stress could lead to diseases such as chronic inflammation and eventually diabetes, cancer, as well as cardiovascular, neurological, and pulmonary diseases. [8] Enzyme systems (e.g., superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase) are responsible for protecting cells from the detrimental effects of ROS by modulating the intracellular ROS level.Nanozyme can also modulate the ROS level in cells (Figure 2). [9] The ROS scavenging abilities of nanozymes largely originate from the SOD mimicking activities, which convert superoxide into H 2 O 2 and subsequently to O 2 and H 2 O, and thus reduce the intracellular ROS level and enhance cell viability. The generation of ROS originates from their peroxidases (POD) activities, which convert H 2 O 2 to OH· radicals, and oxidase activities, which convert O 2 to H 2 O 2 . Outside the cell, ROS can react with biologically active molecules (such as glucose, dopamine, glutathione, and adenosine triphosphate), organic compound (such as phenol and dyes), and pollutants from aqueous solutions. Thus the nanozymes could play important roles in in vitro test and environmental protection. [10] Earlier reports on nanozymes mainly focused on the aspect of experimental study and rarely focused on the theoretical work and mechanism clarification. In this progress report, we first introduce various nanozymes and highlight recent experimental development of new nanozymes that have been investigated to mimic various enzymes. Then we review their applications in various fields including biosensing, bioimaging, therapeutics, and environmental protection. At last, we summarize the catalytic mechanisms and address the challenges of experimental and computational research in this field. The Structure of NanozymesIssues of material design for high catalytical activity, selectivity, and durability are recently accentuated. Among them As a new generation of artificial enzymes, nanozymes have the advantages of high catalytic activity, good stability, low cost, and other unique properties of nanomaterials. Due to their wide range of potential applications, they have become an emerging field bridging nanotechnology and biology, attracting researchers in various fields to design and synthesize highly catalytically active nanozymes. However, the thorough understanding of experimental phenomena and the mechanisms beneath practical applications of nanozymes limits their rapid development. Herein, the progress of experimental and computational...

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Section: The Structure Of Nanozymesmentioning

confidence: 99%

Recent Advances in Nanozyme Research

2018

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“…Previous mechanism studies illuminated that the aerobic oxidation of glucose follows the Eley–Rideal mechanism where glucose is adsorbed on the surface of Au catalysts and then reacts with O 2 . Therefore, the reason why BSA showed nearly negligible effect on the GOx‐like activity may be that the dosage of binding BSA was not enough to inhibit the accessibility of glucose.…”

Section: Resultsmentioning

confidence: 99%

“Non‐Naked” Gold with Glucose Oxidase‐Like Activity: A Nanozyme for Tandem Catalysis

Zhang

Liang

Han

et al. 2018

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It has been widely reported that “naked” gold nanoparticles (Au NPs) without protectors have glucose oxidase (GOx)‐like activity, and the use of protectors can inhibit the GOx‐like activity. Here, “non‐naked” Au NPs with GOx‐like activity are synthesized by using protein as protector. Although “naked” Au NPs have peroxidase‐like activity and GOx‐like activity, the optimal pH ranges of the both activities are obviously different. Fortunately, as‐synthesized “non‐naked” Au NPs show the dual enzyme‐like activities at the same pH. So, “non‐naked” Au NPs can be described as “tandem nanozyme.” As another bonus, the participation of protein protector can stabilize the GOx‐like activity and make Au NPs modifiable. Even though Au NPs are connected with graphene oxide (GO), the GOx‐like activity is still not changed. Further, Au NPs‐GO nanocomposites are applied on the one‐pot nonenzymatic glucose colorimetric detection. The “non‐naked” gold not only broadens the species of tandem nanozymes, but also facilitates the functionalization of nanozymes, which is promising for immunoassay, biosensor, and medical treatment.

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“…However, as these nanomaterials do not have well‐defined binding sites, they are often considered less specific than biological enzymes. A number of nanomaterials have been shown to act as artificial nonbiological enzymes, that is, nuclease, esterase, glucose oxidase, catalase, phosphatase, nitrate reductase, and peroxidase activities of nanomaterials have been reported . Recently, the peroxidase‐like activity of emerging two‐dimensional nanoparticles (2D‐nps), that is, nano‐graphene oxide (nGO) and MoS 2 , has been discovered and exploited for diverse biological applications …”

Section: Introductionmentioning

confidence: 99%

Masking the Peroxidase‐Like Activity of the Molybdenum Disulfide Nanozyme Enables Label‐Free Lipase Detection

et al. 2018

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Two-dimensional MoS nanoparticles (2D-nps) exhibit artificial enzyme properties that can be regulated at bio-nanointerfaces. We discovered that protein lipase is able to tune the peroxidase-like activity of MoS 2D-nps, offering low-nanomolar, label-free detection and identification in samples with unknown identity. The inhibition of the peroxidase-like activity of the MoS 2D-nps was demonstrated to be concentration dependent, and as low as 5 nm lipase was detected with this approach. The results were compared with those obtained with several other proteins that did not display any significant interference with the nanozyme behavior of the MoS 2D-nps. This unique response of lipase was characterized and exploited for the successful identification of lipase in six unknown samples by using qualitative visual inspection and a quantitative statistical analysis method. The developed methodology in this approach is noteworthy for many aspects; MoS 2D-nps are neither labeled with a signaling moiety nor modified with any ligands for signal readout. Only the intrinsic nanozyme activity of the MoS 2D-nps is exploited for this detection approach. No analytical equipment is necessary for the visual detection of lipase. The synthesis of the water-soluble MoS 2D-nps is low costing and can be performed in bulk scale. Exploring the properties of 2D-nps and their interactions with biological materials reveals highly interesting yet instrumental features that offer the development of novel bioanalytical approaches.

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Characterization of glucose oxidation by gold nanoparticles using nanoceria

Cited by 90 publications

References 33 publications

Recent Advances in Nanozyme Research

Recent Advances in Nanozyme Research

“Non‐Naked” Gold with Glucose Oxidase‐Like Activity: A Nanozyme for Tandem Catalysis

Masking the Peroxidase‐Like Activity of the Molybdenum Disulfide Nanozyme Enables Label‐Free Lipase Detection

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