Nucleolar organizer regions in rat urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine

Takeuchi, Toshimi; Tanaka, Takuji; Ohno, Takatoshi; Yamamoto, Naoki; Kobayashi, Satoru; Kuriyama, Manabu; Kawada, Yukimichi; Mori, Hideki

doi:10.1007/bf02890096

Cited by 23 publications

(7 citation statements)

References 14 publications

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“…Since they differ from G3 tumors especially by the nucleolar features, it becomes obvious that morphology and num ber of nucleoli might become important features for the assessment of the proliferative behavior of bladder can cer. This conclusion is supported by reports about a statis tical correlation of the number of nucleolar organizer regions and the proliferative activity of bladder cancer in men and animals [5,24], Future studies have to confirm whether these results can help to improve the current sys tem of grading and to avoid the prognostically inhomo geneous G2 grade. A further result of our analysis is that Gl and G3 tumors are more homogeneous with respect to cytomorphologic variations between different prepara tions.…”

Section: Discussionsupporting

confidence: 50%

Analysis of Criteria for Grading Bladder Cancer in Urine Cytological Tumor Diagnosis by Means of an Expert System

Nafe

Roth²,

Rathert³

1992

Eur Urol

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Section: Discussionsupporting

confidence: 50%

Analysis of Criteria for Grading Bladder Cancer in Urine Cytological Tumor Diagnosis by Means of an Expert System

Nafe

Roth²,

Rathert³

1992

Eur Urol

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“…This may imply that FBRA shows modulating effects in epithelium of animals given carcinogens. Our earlier study indicated a step-wise increase of the AgNOR number from histologically normal transitional epithelium through hyperplasia and pre-cancerous lesions (dysplasia) to transitional cell carcinoma in OH-BBN-induced urinary bladder carcinogenesis (25). Furthermore, some chemopreventive agents reduced AgNOR scores and the development of urinary bladder cancer (9,26).…”

Section: Discussionmentioning

confidence: 92%

Chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran

Kuno

Hirose²,

Yamada³

et al. 2006

Oncol Rep

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Abstract. Fermented brown rice by Aspergillus oryzae (FBRA) has been shown to be a potent anti-carcinogenic compound. Here, we investigated the modifying effects of dietary feeding with a naturally occurring anti-oxidant FBRA on N-butyl-N-(4-hydroxybutyl)-nitrosamine (OH-BBN)-induced urinary bladder carcinogenesis in male ICR mice. Five-week-old male ICR mice were divided into 7 groups, and groups 1-5 were given OH-BBN (500 ppm) in drinking water for 6 weeks starting at 7 weeks of age. Groups 2 and 3 were fed the diet containing 5% and 10% FBRA during the initiation phase, respectively, whereas groups 4 and 5 were fed these diets during the post-initiation phase. Group 6 was given the diet containing 10% FBRA throughout the experiment, and group 7 was kept on the basal diet alone and served as an untreated control. At the end of the study (week 32), the incidences of simple hyperplasia, dysplasia and carcinoma in the bladders of group 1 (OH-BBN alone) were 92%, 49% and 38%, respectively. Those of group 5 (64%, 23% and 10%) and the incidence of carcinoma of group 4 (17%) was significantly less than that of group 1. Furthermore, the multiplicity of simple hyperplasia and carcinoma of group 5 was significantly less than that of group 1. Postinitiation exposure of 10% FBRA significantly decreased the number/nucleus of silver-stained nucleolar organizer region proteins (AgNORs), an index of cell proliferation, in the non-lesional transitional epithelium when compared to that of the control. Our results indicate that FBRA exerts chemopreventive effects against chemically induced urinary bladder carcinogenesis through anti-proliferative mechanisms. FBRA could be a promising chemopreventive agent for human urinary bladder cancer. IntroductionBladder cancer is the 7th most common cancer worldwide, with an estimated 336,000 new cases occurring each year (1). In Japan, the incidence and mortality rates of this malignancy are lower compared with Western populations, but have gradually increased (2,3). The occurrence of neoplasms in the urinary tract epithelium is known to relate to multistage and multifocal carcinogenesis. A high recurrence rate of superficial bladder tumors, even after curative transurethral resection, has often been reported (4). These successive, recurrent tumors may increase in their histological grade, and more than 15% of the patients are predicted to suffer the progression with subsequent poor prognosis (5,6). A number of anticancer drugs have been used, mainly via local instillation into the bladder, as an adjuvant to surgery to suppress or prevent tumor recurrence. However, only a few drugs have been regarded as effective agents. Therefore, a new modality is required to achieve a more satisfactory clinical control of this malignancy.One approach to curb cancer incidence is chemopreventive intervention through which the disease can be totally prevented, delayed, or fixed dormant by the administration of one or more naturally occurring and synthetic chemical agents. Certain synthetic compounds, such...

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“…Increased ribosomal gene transcription has always been considered as a cause of an increase in NORs (2, 53, 69), as recently exemplified by higher AgNOR counts in anterior pituitary corticotrophs of adrenalectomized rats (50). It has recently been shown that AgNOR numbers in tumor cells reflect cell proliferation rather than nuclear ploidy (22,62,63,65), whereas in human non-neoplastic trophoblastic tissue, AgNOR counts were a reflection of ploidy rather than of cell proliferation (61). The alteration of AgNORs seen in doxorubicin-treated rats may reflect a defect of nucleolar association linked to the pharmacology of the drug.…”

Section: Toxicologic Pathologymentioning

confidence: 99%

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

et al. 1991

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ABSTRAC~The use of the argyrophilic (Ag) staining technique for nucleolar organizer regions (NORs) revealed nuclear changes in myocytes of the left atrium of 10 rats treated twice a week for 6 weeks with doxorubicin (1 mg/ kg body weight) iv and sacrificed after 6 weeks without treatment. The changes were easily detected qualitatively and further assessed by quantification. Cardiac myocytes of doxorubicin-treated rats had larger nuclei and/or a larger quantity of AgNORs that were either dispersed in a number of small dots or clustered in rounded, rod-shaped, or tortuous large structures. AgNOR alterations may reflect a defect of nucleolar association leading to an impairment ofprotein synthesis that could be involved in doxorubicin cardiotoxicity.

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Nucleolar organizer regions in rat urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine

Cited by 23 publications

References 14 publications

Analysis of Criteria for Grading Bladder Cancer in Urine Cytological Tumor Diagnosis by Means of an Expert System

Analysis of Criteria for Grading Bladder Cancer in Urine Cytological Tumor Diagnosis by Means of an Expert System

Chemoprevention of mouse urinary bladder carcinogenesis by fermented brown rice and rice bran

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

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